[1]杨依骏,杜建钢,朱琴芳,等.系统性红斑狼疮患者血清IL-10和IL-17水平检测的临床意义[J].现代检验医学杂志,2017,32(03):71-73，78.[doi:10.3969/j.issn.1671-7414.2017.03.019]
　YANG Yi-jun,DU Jian-gang,ZHU Qin-fang,et al.Clinical Significance of Serum IL-10 and IL-17 in Patients with Systemic Lupus Erythematosus[J].Journal of Modern Laboratory Medicine,2017,32(03):71-73，78.[doi:10.3969/j.issn.1671-7414.2017.03.019]
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系统性红斑狼疮患者血清IL-10和IL-17水平检测的临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
71-73，78
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Clinical Significance of Serum IL-10 and IL-17 in Patients with Systemic Lupus Erythematosus
文章编号:
1671 -7414(2017)03-071-04
作者:
杨依骏; 杜建钢; 朱琴芳; 俞 钱; 徐红星
南京医科大学附属苏州市立医院本部检验科，江苏苏州 215002
Author(s):
YANG Yi-jun; DU Jian-gang; ZHU Qin-fang; YU Qian; XU Hong-xing
Department of Clinical Laboratory， Suzhou Municipal Hospital，Nanjing Medical University，Jiangsu Suzhou 2150 02，China
关键词:
系统性红斑狼疮; 白细胞介素-10; 白细胞介素-17
分类号:
R593.241；R392.11
DOI:
10.3969/j.issn.1671-7414.2017.03.019
文献标志码:
A
摘要:
目的 通过系统性红斑狼疮(SLE)患者血清中IL-10和IL-1 7的水平，探讨SLE患者炎症反应情况及Treg细胞与Th17细胞在SLE发病中的作用。〖HT5”H 〗方法 选取2013年7月～2015年7月南京医科大学附属苏州市立医院本部临床已 经确诊的SLE患者54例为研究对象，同期健康体检者19例为对照组，采用酶联免疫吸附试验( ELISA)双抗体夹心法检测两组血清IL-10和IL-17水平，采用间接免疫荧光法及免疫印迹法 测定SLE患者ANA，dsDNA及RNP，Sm，SSA，SSB，并对IL-10和IL-17水平进行比较及相关性 分析，同时分析ANA，RNP，Sm，SSA和dsDNA分组IL-10和IL-17的水平差异。结 果 SLE患者IL-10和IL-17水平为63.7±89.0 pg/ml和87.7±123.0 pg/ml ，和对照组20.8±8.9 pg/ml和18.5±111.6 pg/ml比较，差异有统计学意义(t IL-10=3.484，tIL-17=4.076，P均<0.01)；SLE患者中IL-10和IL -17阳性组结果为176.5±93.2　pg/ml和237.2±107.5　pg/ml，阴性组为16.2±5.7 　pg/ml和12.9±8.3　pg/ml，差异有统计学意义(tIL-10=6.875，t IL-17=8.843，P均<0.01)；SLE患者IL-10与IL-17水平呈正相关关系(r=0. 96，P<0.05)。ANA高滴度组IL-10和IL-17水平为91.8±100.8　pg/ml和135.5±140 .9　pg/ml，ANA低滴度组为44.5±76.7　pg/ml和54.4±98.5　pg/ml，差异有统计学 意义(tIL-10=2.215，tIL-17=2.345，P均<0.05)。抗ds-D NA抗体阳性组IL-10和IL-17水平为87.1±101.1 pg/ml和122.4±137.1　pg/ml，抗ds -DNA抗体阴性组为27.4±50.1 pg/ml和28.6±61.6　pg/ml，差异有统计学意义(t IL-10=2.904，tIL-17=3.443，P均<0.05)。结论 系统性红斑狼疮患者的抗炎细胞因子IL-10和促炎细胞因子IL-17的高水平及高 度正相关，说明SLE患者抗炎和促炎反应同时存在，SLE患者Treg细胞与Th17细胞存在失衡， 可能在SLE的发生和发展中同时起重要作用。
Abstract:
Objective To investigate the effect of Treg cel ls and Th17 cells on the pathogenesis of SLE in patients with SLE，by detecting levels of serum IL-10 and IL-17 in patients with systemic lupus erythematosus (SLE)．Methods Selected 54 patients with SLE diagnosed in Suzhou Municipal Hospital Affiliated to Nanjing Medical University from July 20 13 to July 2015 as subjects，and 19 healthy persons were selected as control gro up．Using double antibody sandwich method (ELISA) to detect the levels of IL-10 and IL-17 in two groups．Using indirect immunofluorescence and western blot me thod to detect ANA，dsDNA，RNP，Sm，SSA and SSB in patients with SLE．Then comp ared and correlation analysed the level of IL-10 and IL-17，the levels of IL- 10 and IL-17 in ANA，RNP，Sm，SSA and dsDNA groups were analyzed simultaneously ．Results The level of IL-10 and IL-17 in SLE were 63. 7±89.0 pg/ml and 87.7±123.0 pg/ml，and control group were 20.8±8.9 pg/ml and 18.5±111.6 pg/ml，with the statistically significant difference (t IL-10=3.484，tIL-17=4.076，P<0.01)．The level of positive group in SLE were 176.5±93.2　pg/ml and 237.2±107.5　pg/ml，and negative group were 16.2±5.7　pg/ml and 12.9±8.3　pg/ml，with the statistically sig nificant difference (tIL-10=6.875，tIL-17=8.843，P<0 .01)．The level of IL-10 was positively correlated with IL-17 level in SLE ( r=0.96，P<0.05)．The level of IL-10 and IL-17 in ANA high titer group we re 91.8±100.8　pg/ml and 135.5±140.9　pg/ml，ANA low titer group were 44. 5±76.7　pg/ml and 54.4±98.5　pg/ml，with the statistically significant diff erence (tIL-10=2.215，tIL-17=2.345，P<0.05)．The le vel of IL-10 and IL-17 in anti ds-DNA antibody positive group were 87.1±101 .1　pg/ml and 122.4±137.1　pg/ml，negative group were 27.4±50.1　pg/ml an d 28.6±61.6　pg/ml，with the statistically significant difference (tI L-10=2.904，tIL-17=3.443，P<0.05)．Conclusion The levels of IL-10 and IL-17 were significantly increased and ther e was positive correlation．It was explained that the anti-inflammatory and pro -inflammatory response existing at the same time in SLE patients and Treg cell and Th17 cell may also play an important role in the occurrence and development in SLE．

参考文献/References:

［1］ 蒋巧雅，迟秀文，蓝宇萍．系统性红斑狼疮血清IL-17和ICAM-1的检测及 其免疫学意义［J］．现代检验医学杂志，2012，27(3)：74-76． Jiang QY，Chi XW，Lan YP．Immunological significance of the detectio n of IL-17 and ICAM -1 in systemic lupus erythematosus［J］．J Mod Lab Med，20 12，27(3)：74-76．
［2］ 马纪林，蔡 龙，施华萍，等．系统性红斑狼疮患者外周血调节性T细胞与Th17细胞 失衡的研究［J］．医学研究杂志，2013，42(3)：27-30． Ma JL，Cai L，Shi HP，et a1．Study on the imbalance CD4+CD25high regulatory T cells/Th17 cells in the peripheral blood of patients with system ic lupus erythematosus［J］．J Med Res，2013，42(3)：27-30．
［3］ Feldman CH， Hiraki LT， Liu J，et al．Epidemiology and sociodemographics of systemic lupus erythematosus and lupus nephritis among U．S．adults with med icaid coverage，2000-2004［J］．Arthritis & Rheumatology，2013，65(3)：753-760 ．
［4］ 严静霞，高丽霞，吴 霞，等．Th17细胞在系统性红斑狼疮伴发心血管疾病中的意 义探讨［J］．现代检验医学杂志，2014，29(6)：52-54，58． Yan JX，Gao LX，Wu X，et al．Evaluate the significance of Th17 cells in systemic lupus erythematosus with cardiovascular disease［J］．J Mod Lab Med ，2014，29(6)：52-54，58．
［5］ Josefowicz SZ，Lu LF，Rudensky AY．Regulatory T cells：mechanisms of dif ferentiation and function［J］．Annu Rev Immunol，2012(3)：531-564．
［6］ Song X，Li B，Xiao Y，et al．Structural and biological features of Foxp3 dimerization relevant to regulatory T cell function［J］．Cell Reports，2012，1( 6)：665-675．
［7］ Lina C，ConghHa W，Nan L，et al．Combined treatment of etanercept and MTX reverses Thl/Th2，Thl7/Treg imbalance in patients with rheumatoid arthritis［J ］．J Clin Immunol，2011，31(4)：596-605．
［8］ 汤雯剑，罗 浩，赵金涛，等．银屑病患者血清中IL-10与IL-21水平监测的临床 应用［J］．现代检验医学杂志，2015，30(2)：65-67． Tang WJ，Luo H，Zhao JT，et al．Clinical application of IL-10 and I L-21 levels monitoring in patients with psoriasis［J］．J Mod Lab Med，2015，30 (2)：65-67．
［9］ 付 丹，李成荣，何颜霞，等．婴幼儿脓毒症不同免疫状态细胞因子变化探讨［J］ ．中国小儿急救医学，2009，40(5)：438-440． Fu D，Li CR，He YX，et al．Investigation of the changes of cytokines in infants with immunological activated or immunosuppressed status of sepsis［J ］．Chin Pediatr Emerg Med，2009，40(5)：438-440．
［10］ 马东来，张少静，文夫瑞德·斯特克．自身抗体及其免疫荧光模式［M］ ．北京：北京科学技术出版社，2000：8． Ma DL，Zhang SJ，Winfried Stocker．Autoantibodies and their immunofluoresc ent patterns［M］．Beijing：Beijing Science Technology Press，2008：8．

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备注/Memo

备注/Memo:

作者简介：杨依骏(1991-)，女，大学本科，检验技师，从事临床检验研究，Te l：0512-62362379，E-mail：441726930@qq.com。
通讯作者：杜建钢(1977-)，男，大学本科，副主任技师，从事临 床检验研究，Tel：0512-62362370，E-mail：djg0320@163.com。
更新日期/Last Update: 2017-06-25