[1]黄 芳a,靳耀峰b,卢 杰a,等.应用免疫组化方法检测天疱疮患者皮肤组织热休克蛋白70的临床研究[J].现代检验医学杂志,2017,32(03):82-85.[doi:10.3969/j.issn.1671-7414.2017.03.022]
 HUANG Fanga,JIN Yao-fengb,LU Jiea,et al.Clinical Study on Heat Shock Protein 70 in Skin Tissue of Patients with Pemphigus by Immunohistochemistry[J].Journal of Modern Laboratory Medicine,2017,32(03):82-85.[doi:10.3969/j.issn.1671-7414.2017.03.022]
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应用免疫组化方法检测天疱疮患者皮肤组织热休克蛋白70的临床研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
82-85
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Clinical Study on Heat Shock Protein 70 in Skin Tissue of Patients with Pemphigus by Immunohistochemistry
文章编号:
1671-7 414(2017)03-082-04
作者:
黄 芳1a靳耀峰1b卢 杰1a李思鹏 1a张 婷1a冯义国1c陈静宏2耿 燕1a
1.西安交通大学第二附属医院a.检验科;
b.病理科;
c.皮肤科,西 安 710004;
2.西安交通大学 环境与疾病相关教育部重点实验室暨卫生部微量元素与地方病重点实验室,西安 71 0048
Author(s):
HUANG Fang1aJIN Yao-feng1bLU Jie1a LI Si-peng1aZHANG Ting1aFENG Yi-guo1c CHEN Jing-hong2GENG Yan1a
1a.Department of Clin ical Laboratory;
1b.Department of Clinical Pathology;
1c.Department of Dermatolog y, the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 71000 4,China;
2.Institute of Endemic Diseases,Medical School of Xi’
关键词:
天疱疮热休克蛋白70发病机制
分类号:
R758.66;R446.8
DOI:
10.3969/j.issn.1671-7414.2017.03.022
文献标志码:
A
摘要:
目的 通过对天疱疮患者皮肤组织热休克蛋白70(HSP70)蛋 白表达水平的测定,探讨HSP70在皮肤组织表达与天疱疮发病的关系,为进一步阐明天疱疮 的发病机制提供新的理论依据。方法 采用免疫组化方法测定62例天 疱疮患者皮肤组织HSP70,以正常皮肤组织作为对照进行HSP70测定。结果 〖HT 5”K〗天疱疮患者水泡部位HSP70表达阳性细胞数>75%,相比正常皮肤HSP70的表达差异有统 计学意义(Z=5.42,P<0.01),天疱疮患者炎性部位HSP70表达阳性细胞数>50%,相比 正常皮肤HSP70的表达差异有统计学意义(Z=4.73,P<0.01)。结论 〖HT 5”K〗天疱疮患者水泡部位以及水泡附近炎性细胞HSP70的异常增高表达,为HSP70参与天疱 疮疾病的发生和发展提供了直接的证据。
Abstract:
Objective This study was performed to investiga te the levels of HSP70 in tissue in pemphigus as a possible new theoretical basi s for further elucidate the pathogenesis of pemphigus.Methods The expression of HSP70 in 62 patients with pemphigus was determined by im munohistochemistry,and the normal skin was taken as control.Results The results showed that the positive cells of HSP70>75% in the blis ters of pemphigus vulgaris and the positive cells of HSP70>50% in the inflammato ry cells near the blisters,and the expression of HSP70 was significantly higher than that in normal skin,which was statistically significant(Z=5.42,4.73 ,P<0.01).Conclusion The abnormal expression of HSP7 0 in inflammatory cells and psoriasis of pemphigus patients showed that HSP70 is involved in the pemphigus.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:陕西省卫生厅科研基金(2012D67)。
作者简介:黄 芳(1968-),女,硕士,副主任技师 ,研究方向:自身免疫病的检测,Tel:029-87679344,E-mail:huangfang135720@163.c om。
通讯作者:陈静宏,E-mail: jixiang46@163.com。 耿 燕,E-mail: wsw87679358@163.com。
更新日期/Last Update: 2017-06-25