[1]李 娅,章 迪,张 赟,等.罗氏cobas e602与modular e170电化学发光分析仪检测血清HBsAg阳性低值结果的对比分析[J].现代检验医学杂志,2017,32(03):123-125.[doi:10.3969/j.issn.1671-7414.2017.03.033]
　LI Ya,ZHANG Di,ZHANG Yun,et al.Study on the Low Positive Results of Detecting HBsAg in Serum Specimens with Roche Cobas e602 and Modular e170 Electrochemical Luminescence Analyzer[J].Journal of Modern Laboratory Medicine,2017,32(03):123-125.[doi:10.3969/j.issn.1671-7414.2017.03.033]
点击复制

罗氏cobas e602与modular e170电化学发光分析仪检测血清HBsAg阳性低值结果的对比分析()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
123-125
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Study on the Low Positive Results of Detecting HBsAg in Serum Specimens with Roche Cobas e602 and Modular e170 Electrochemical Luminescence Analyzer
文章编号:
1671-7414 (2017)03-123-03
作者:
李 娅1，章 迪2，张 赟1，皇 海3，苏明权3
1.西安市第四医院检验科，西安 710004；
2.西安市中心血站，西安 710061；
3.第四军医大学西京医院全军检验医学研究所，西安 710032
Author(s):
LI Ya1，ZHANG Di2，ZHANG Yun1，HUANG Hai2，SU M ing-quan2
1.Department of Clinical Laboratory，the Fourth Hospital in Xi ’an，Xi’an 710004，China；
2.Blood Center of Xi’an,Xi’an 710061，China；
3.Research Institute of Clinical Laboratory Medicine of PLA，Xijing Hospital，Fourth Military Medical University，Xi’
关键词:
乙肝表面抗原; 罗氏Cobas e602; 罗氏Modular e170; 阳性低 值
分类号:
R512.62；R446
DOI:
10.3969/j.issn.1671-7414.2017.03.033
文献标志码:
A
摘要:
目的 探讨乙肝表面抗原(HBsAg)阳性低值结果在不同检测系 统间结果的相关性，为临床检验结果分析及报告的准确发布提供参考。方法 〖 HT5”K〗选取经罗氏电化学发光检测仪Cobas e602检测的HBsAg结果在1～80 COI的标本243 份，同时将标本在罗氏电化学发光检测仪Modular e170上进行检测，计算两组测定值的直线 回归相关系数R2值，计算各组别的阳性符合率。结果 两个检 测系统比对实验结果为R2=0.933。根据测定值高低分为A，B，C，D和E组的阳性符合 率分别为60.60%，92.72%，96.66%，96.66%和100%；男性组阳性符合率为87.66%，女 性组阳性符合率为70.96%，男性患者阳性符合率大于女性患者(P<0.05)。 结论 两检测系统对于范围在1～80 COI的标本检测结果有相关性：其中测量值 在10 COI以上标本的阳性符合率完全一致；测量值在1～10 COI的标本阳性符合率不一致， 且随结果的减小而降低，在临床上可能导致有争议的结果，建议做进一步复查。
Abstract:
Objective To investigate the correlation betwe en the results of HBsAg positive results among different detection systems，and provide reference for the analysis of clinical test results and the publication of the report．Methods The HBsAg positive serum specimen s with the quantitation result lower than 80 COI were detected by Roche Cobas e6 02 electrochemical luminescence analyzer．All the specimens were also detected b y Roche Modular e170 electrochemical luminescence analyzer．The differences of d etecting results were compared and performed the linear correlation analysis．〖 WT5”HZ〗Results The experimental results of two detection systems a re R2=0.933．The positive coincidences of Group A，B，C，D and E were 60 .60%，92.72%，96.66%，96.66% and 100%，respectively．The positive coincidenc e of the males was 87.66%，while the positive coincidence of the females was 70 .96%．The positive coincidence of the males was significantly higher than the f emales (P<0.05)．Conclusion The HBsAg detecting resu lts of Roche Cobas e602 and Roche Modular e170 electrochemical luminescence anal yzer had high correlation．The results higher than 10 COI had 100% positive coin cidence rate，however the results between 1 and 10 COI were not．The result betw een 1 and 10 COI may lead to controversial results，suggestions for further chec ks．

参考文献/References:

［1］ Lu FM，Zhuang H．Management of hepatitis B in China［J］．Chin Me d J (Engl)，2009，122(1)，122：3-4．
［2］ 高春芳．HBV S区基因变异与HBsAg检测［J］．中华检验医学杂志，2010，33(6)：5 74-576． Gao CF．Genetic variability of the HBV sgene and HBsAg detection［J ］．Chinses Journal of Laboratory Medicine，2010，33(6)：574-576．
［3］ 刘 杨，彭道荣．乙肝表面抗原实验室检测方法的进展［J］．国际检验医学杂志， 2015，36(17)：2546-2548． Liu Y，Peng DR．Hepatitis b surface antigen progress of laboratory t esting method［J］．International Journal of Laboratory Medicine，2015，36(17)： 2546-2548．
［4］ Shi Y，Zeng W，Wang N，et al．A novel fMRI group data analysis method bas ed on data-driven reference extracting from group subjects［J］．Comput Methods Programs Biomed，2015，122(3)：362-371．
［5］ Esteve-Turrillas F， Agulló C， Abad-Somovilla A，et al．Fungicide mul tiresidue monitoring in international wines by immunoassays［J］．Food Chem，201 6(196)：1279-1286．
［6］ Spasovski D，Masin-Spasovska J，Nada M，et al．Diagnostic value of brush border enzymes of the proximal renal tubules in rheumatoid arthritis［J］．Clin Lab，2011，57(5/6)：305-314．
［7］ Daly JM，Bay CP，Xu Y，et al．Effect of ambient temperature variations on positivity of manual fecal immunochemical tests［J］．J Prim Care Community Hea lth，2015，6(4)：243-249．
［8］ 何紫琪，李从荣，尹 路．乙型肝炎病毒逆转录酶基因突变的临床意义［J］．现代 检验医学杂志，2016，31(4)：83-86． He ZQ，Li CR，Yin L．Clinical significance of reverse transcriptase gene mutations in the hepatitis B virus［J］．Journal of Modern Laboratory Medic ine，2016，31(4)：83-86．
［9］ 中国合格评定国家认可委员会．医学实验室质量和能力认可准则［S］．北京：中国 合格评定国家认可委员会，CNAS-CL02：2012． China National Accreditation Service for Conformity Assessment．Accr editation critrreria for the Quality and competence of Medical Laboratories［S］ ．Beijing:China National Accreditation Service for Conformity Assessment，CNAS- CL02：2012．

相似文献/References:

[1]刘宗萍.慢性乙肝患者血清 Tim-3，HBsAg，TGF-β1水平表达与病情程度相关性研究[J].现代检验医学杂志,2019,34(06):77.[doi:10.3969 ／ j.issn.1671-7414.2019.06.019]
　LIU Zong-ping.Study on the Correlation between the Expression of Serum Tim-3, HBsAg, TGF-beta 1 and the Severity of Chronic Hepatitis B[J].Journal of Modern Laboratory Medicine,2019,34(03):77.[doi:10.3969 ／ j.issn.1671-7414.2019.06.019]
[2]杨 阳,张欢妍,吕 幸,等.筛选血清HBV-DNA 阴性且HBsAg 与HBsAb 阳性CHB 患者的血清危险因素及评分与预后的相关性研究[J].现代检验医学杂志,2023,38(06):153.[doi:10.3969/j.issn.1671-7414.2023.06.028]
　YANG Yang,ZHANG Huanyan,L? Xing,et al.Correlation of Screening for Serum Risk Factors and Risk Factor Score to Prognosis between DNA-negative and Co-existing with HBsAg/HBsAb in CHB Patients[J].Journal of Modern Laboratory Medicine,2023,38(03):153.[doi:10.3969/j.issn.1671-7414.2023.06.028]
[3]倪 勇,陈丹丹,龚美娜,等.两种化学发光免疫分析系统对血清HBsAg突变株的检测能力比较分析[J].现代检验医学杂志,2025,40(03):195.[doi:10.3969/j.issn.1671-7414.2025.03.036]
　NI Yong,CHEN Dandan,GONG Meina,et al.Comparative Analysis of Two Chemiluminescent Immunoassay Systems for the Detection of Serum HBsAg Mutants[J].Journal of Modern Laboratory Medicine,2025,40(03):195.[doi:10.3969/j.issn.1671-7414.2025.03.036]

备注/Memo

备注/Memo:

作者简介：李 娅(1980-)，女，硕 士研究生，主管检验师，研究方向：分子生物学，Tel：029-87480873，E-mail：yaya502 11cn@sina.com。
更新日期/Last Update: 2017-06-25