[1]蒋晶慧,于洪伟,张 泽,等.基于石墨烯量子点构建生物传感器检测尿酸和多巴胺的实验研究[J].现代检验医学杂志,2017,32(04):9-11.[doi:10.3969/j.issn.1671-7414.2017.04.003]
　JIANG Jing-hui,YU Hong-wei,ZHANG Ze,et al.Simultaneous Electrochemical Detection of Uric Acid and Dopamine Based on Graphene Quantum Dot Modified Glassy Carbon Electrode[J].Journal of Modern Laboratory Medicine,2017,32(04):9-11.[doi:10.3969/j.issn.1671-7414.2017.04.003]
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基于石墨烯量子点构建生物传感器检测尿酸和多巴胺的实验研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
9-11
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Simultaneous Electrochemical Detection of Uric Acid and Dopamine Based on Graphene Quantum Dot Modified Glassy Carbon Electrode
文章编号:
1671-7414(2017)04-009-03
作者:
蒋晶慧1; 于洪伟2; 张 泽2; 常 东1
1.哈尔滨医科大学附属第一医院检验科,哈尔滨 150001; 2.复旦大学附属浦东医院检验科,上海 200120
Author(s):
JIANG Jing-hui1; YU Hong-wei2; ZHANG Ze2; CHANGDong1
1.Department of Clinical Laboratory,the First Affiliated Hospital of the Harbin Medical University,Harbin 150001,China; 2.Department of Clinical Laboratory, Pudong Hospital Affiliated to Fudan University,Shanghai 200120,China
关键词:
石墨烯量子点;  生物传感器;  尿酸;  多巴胺
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2017.04.003
文献标志码:
A
摘要:
目的 制备石墨烯量子点(GQDs)以构建生物传感器检测尿酸和多巴胺。方法 一步法碳化枸橼酸合成含羧基的石墨烯量子点用以构建电化学生物传感器,以差分脉冲法检测尿酸和多巴胺,并评估其检测性能。采集2016年7月29日三名实验人员晨尿,用构建的传感器以完成生物样本检测。结果 透射电镜观察到制备的GQDs大小在3～5 nm之间。构建的生物传感器检测50～600 μmol/L尿酸(r2=0.996 6)和2～240 μmol/L多巴胺(r2=0.992 5)的浓度线性相关性良好。检测尿液样本中尿酸的浓度(n=3),RSD值小于3%。尿酸和多巴胺的加标回收率范围分别在95.7%～101.4%和96%～102%。结论 成功构建了基于石墨烯量子点的生物传感器检测尿酸和多巴胺。
Abstract:
Objective To preparegraphene quantum dots(GQDs)and construct a novel biosensor for determination of dopamine(DA)and uric acid(UA).Methods The GQDs was prepared by carbonization method from citric acid as carbon sources.Differential pulse voltammetry was used by the modified electrodes to detect uric acid and dopamine,and the detectionperformance was evaluate.Collected three experimenters morning urine on July 29,2016.The proposed sensor was used for biological samples detection.Results The size of GQDs were between 3 to 5 nm,which was observed by transmission electron microscopy(TEM).The proposed sensor had good linear correlation of 50～600 μmol/L UA(r2=0.996 6)and 2～240 μmol/L DA(r2=0.992 5).In uric acid in urine samples' detection(n=3), RSD value was less than 3%.The standard addition recovery rates of UA and DA were in95.7%～101.4% and 96%～102% respectively.Conclusion The biosensor based on GQDs for determination DA and UA had been constructed successfully.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81573200)(81373047),上海市浦东新区领先人才培养计划(PWRl2016-04)。 作者简介:蒋晶慧(1990-),女,硕士在读,临床检验诊断学,研究方向:分子生物学,生物传感器,E-mail:410882656@qq.com。 通讯作者:常 东(1969-),女,博士研究生,主任医师,研究方向:分子生物学,生物传感器,哈尔滨医科大学教授,复旦大学附属浦东医院检验科主任,E-mail:dongchang1969@163.com。
更新日期/Last Update: 1900-01-01