[1]李腾达,龙曙萍,黄元兰,等.CD69+CD4+CD25-T在自身免疫性胰腺炎患者外周血中表达升高及临床意义[J].现代检验医学杂志,2017,32(04):29-31.[doi:10.3969/j.issn.1671-7414.2017.04.008]
　LI Teng-da,LONG Shu-ping,HUANG Yuan-lan,et al.Elevated Expression of CD69+CD4+CD25-T Cells in Peripheral Blood from Patients with Autoimmune Pancreatitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(04):29-31.[doi:10.3969/j.issn.1671-7414.2017.04.008]
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CD69+CD4+CD25-T在自身免疫性胰腺炎患者外周血中表达升高及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
29-31
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Elevated Expression of CD69+CD4+CD25-T Cells in Peripheral Blood from Patients with Autoimmune Pancreatitis and Its Clinical Significance
文章编号:
1671-7414(2017)04-029-03
作者:
李腾达1; 龙曙萍1; 黄元兰2; 孙 懿1; 郭 杰1; 刘 云1; 张薇薇1; 谷明莉1; 邓安梅1
1.第二军医大学长海医院检验科,上海 200433; 2.解放军455医院,上海 200052
Author(s):
LI Teng-da1; LONG Shu-ping1; HUANG Yuan-lan2; SUN Yi1; GUO Jie1;  LIU Yun1; ZHANG Wei-wei1; GU Ming-li1; DENG An-mei1
1.Department of Clinical Laboratory,Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433,China; 2.No.455 Hospital of PLA,Shanghai 200052,China
关键词:
自身免疫性胰腺炎;  CD69+CD4+CD25-T细胞;  转化生长因子-β;  白细胞介素-4;  干扰素-γ
分类号:
R576; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.04.008
文献标志码:
A
摘要:
目的 检测CD69+CD4+CD25-T细胞在自身免疫性胰腺炎(AIP)患者外周血中的表达情况,进一步分析其临床意义。方法 收集2014年9月～2016年12月长海医院血液科诊断的32例AIP患者与同期体检的32例健康个体外周血分别作为实验组与对照组。采用密度梯度离心法分离外周血单个核细胞(PBMCs),用流式细胞术检测PBMCs中的CD69+CD4+CD25-T,以ELISA法检测血浆中细胞因子的表达状况。两组间变量的比较采用两独立样本t检验,两计量资料间的关系以Pearson相关系数表示。结果 实验组与对照组CD69+CD4+CD25-T表达水平为10.36%±3.68% vs 3.99%±1.45%,(t=9.110,P<0.000 1); TGF-β的表达水平为399.86±121.88 vs 143.87±56.22 pg/ml,(t=10.79,P<0.000 1),差异均具有统计学意义。实验组CD69+CD4+CD25-T细胞与TGF-β呈正相关(r=0.653,P<0.001),与IL-4,IFN-γ,IL-2呈负相关(r=-0.442,-0.567,-0.351,P<0.05),差异具有统计学意义。结论 CD69+CD4+CD25-T细胞可能参与了AIP的免疫病理过程,是临床诊断与治疗潜在的生物学指标。
Abstract:
Objective To test the expression level of CD69+CD4+CD25-T cells in peripheral blood from patients with autoimmune pancreatitis,and further analyze its clinical significance.Methods Peripheral blood samples from 32 patients with AIP diagnosed in hematological department,Changhai Hospital and 32 health individuals examined at the same timewere collected from September 2014 to December 2016,they were classified as experimental and control groups,separately.Peripheral blood mononuclear cells(PBMCs)was acquired by density gradient centrifugation,CD69+CD4+CD25-T cells inPBMCs were tested by flow cytometry,and the expression level of cytokines in plasm was by ELISA.The comparison of varies between the two groups was measuredby two independent samples' t test.The relationship between the two measurement data was measured by pearson correlation coefficient.ResultsThe expression levels of CD69+CD4+CD25-T in experimental and controlgroups were 10.36%±3.68% vs 3.99%±1.45%(t=9.110,P<0.0001).The expression level of TGF-β was 399.86±121.88 vs 143.87±56.22 pg/ml(t=10.79,P<0.000 1),both with statistical significance.The levels of CD69+CD4+CD25-T in experimental was positively correlated with TGF-β(r=0.653,P<0.001)and negatively with IL-4,IFN-γ,IL-2(r=-0.442,-0.567,-0.351,P<0.05)and there was statistical significance.ConclusionCD69+CD4+CD25-T cells might involve the immunopathology of AIP andcould be the potential biomarker for clinical diagnosis and therapy.

参考文献/References:

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[7] 李腾达,刘挺挺,吴林洪,等.原发性胆汁性肝硬化患者外周血单个核细胞中Tim-1表达增高及其意义[J].现代检验医学杂志,2016,31(4):41-43. Li TD,Liu TT,Wu LH,et al.Increasing expression of Tim-1 in peripheral blood mononuclear cell of patients with primary biliary cirrhosis and itssignificance[J].Journal of Modern Laboratory Medicine,2016,31(4):41-43.
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相似文献/References:

[1]李腾达,龙曙萍,黄元兰,等.自身免疫性胰腺炎患者血清外泌体中CD69表达升高及意义[J].现代检验医学杂志,2018,33(02):8.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　LI Teng-da,LONG Shu-ping,HUANG Yuan-lan,et al.Elevated Expression of CD69 in Serum Exosomes from Patients with Autoimmune Pancreatitis and Its Significance[J].Journal of Modern Laboratory Medicine,2018,33(04):8.[doi:10.3969/j.issn.1671-7414.2018.02.001]

备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81671556,81601406,81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:李腾达(1990-),女,在读硕士生,主要从事感染免疫研究,E-mail:tengdali@smmu.edu.cn。 龙曙萍(1994-),女,在读硕士生,主要从事感染免疫研究,共同第一作者。 通讯作者:邓安梅,女,教授,E-mail: amdeng70@163.com. 谷明莉,女,检验技师,E-mail:mingligu@126.com,共同通讯作者。
更新日期/Last Update: 1900-01-01