[1]赵 枰,洪煜婧,陈 相,等.上皮性卵巢癌患者外周血循环肿瘤细胞检测的临床意义[J].现代检验医学杂志,2017,32(04):50-51,56.[doi:10.3969/j.issn.1671-7414.2017.04.014]
　ZHAO Ping,HONG Yu-jing,CHEN Xiang,et al.Clinical Significance of Circulating Tumor Cells Detection from Peripheral Blood of Patients with Epithelial Ovarian Cancer[J].Journal of Modern Laboratory Medicine,2017,32(04):50-51,56.[doi:10.3969/j.issn.1671-7414.2017.04.014]
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上皮性卵巢癌患者外周血循环肿瘤细胞检测的临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
50-51,56
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Clinical Significance of Circulating Tumor Cells Detection from Peripheral Blood of Patients with Epithelial Ovarian Cancer
文章编号:
1671-7414(2017)04-050-03
作者:
赵 枰1; 洪煜婧2; 陈 相1; 周 峰1; 鲁晓燕1; 洪 宏3; 李 晗1
1.南通市第一人民医院,江苏南通 226001; 2.南通大学公共卫生学院预防医学141班,江苏南通 226019; 3.南通市中医院,江苏南通 226001
Author(s):
ZHAO Ping1; HONG Yu-jing2; CHEN Xiang1; ZHOU Feng1; LU Xiao-Yan1; HONG Hong3; LI Han1
1.Department of Clinical Laboratory,the First People's Hospital in Nantong, Jiangsu Nantong 226001,China; 2.Department of Preventive Medicine,School of Public Health,Nantong University,Jiangsu Nantong 226019,China; 3.Nangtong Hospital of Traditional Chinese Medicine,Jiangsu Nantong 226001,China
关键词:
循环肿瘤细胞;  上皮性卵巢癌
分类号:
R737.31; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.04.014
文献标志码:
A
摘要:
目的 评估循环肿瘤细胞检测对上皮性卵巢癌的诊断价值,并探讨上皮性卵巢癌患者循环肿瘤细胞与临床病理特征的关系。方法 应用免疫磁珠为基础的阴性筛选结合流式细胞术检测南通市第一人民医院65例上皮性卵巢癌患者、21例卵巢良性疾病患者及10例健康者外周血循环肿瘤细胞。多组比较采用Kruskal-Wallis H检验,两组比较采用Mann-Whitney U检验,阳性率比较采用卡方检验,并绘制ROC曲线分析。结果 根据受试者工作特性曲线分析:外周血循环肿瘤细胞的临界值为4.5(>4)个时,其AUC为0.806,诊断上皮性卵巢癌的灵敏度、特异度和阳性预测值分别为55.4%,96.8%和97.3%; 外周血循环肿瘤细胞含量与上皮性卵巢癌患者的年龄、组织学类型、病理分化程度、腹腔积液量、肿瘤大小以及有无淋巴结转移无关(均P>0.05),而与肿瘤的国际妇产科联盟(FIGO)分期和远处转移有关(均P<0.05)。结论 外周血循环肿瘤细胞检测对上皮性卵巢癌的诊断有一定的价值,与FIGO分期和存在远处转移相关。
Abstract:
Objective To estimate the diagnostic vaule of circulating tumor cells(CTCs)detection for epithelial ovarian cancer,and investigate the relationship between the presence of CTCs and the clinic pathologic characteristics of epithelial ovarian cancer patients.Methods Quantification of CTCs were performed using immunomagnetic bead based negative enrichment combined with flow cytometry in 65 patients with epithelial ovarian cancer,21 patients with benign ovarian diseases,and 10 healthy subjects.The differences among groups were analyzed by the Kruskal-Wallis H test(multi group comparison)and the Mann-Whitney U test(two group comparison),and the chi-square test was used in the positive rate comparison,the receiver operating characteristic(ROC)curve was established.The relationship between CTCs and clinic pathologic characteristics of epithelial ovarian cancer patients were analyzed.Results The receiver operating characteristic analysis showed the cut off value was 4.5(>4),the AUC was 0.806,and sensitivity,specificity and positive value(PPV)were 55.4%,96.8% and 97.3% respectively,in detecting patients with ovarian carcinoma malignancy.Quantification of CTCs in epithelial ovarian cancer was correlated with FIGO stages or distance metastasis(all P<0.05),but not with patient age,histological types,pathologic differentiation,amount of ascites,tumor size and lymph node metastasis(all P>0.05).Conclusion The detection of peripheral CTCs has a certain diagnosis value in epithelial ovarian cancer,especially with related to FIGO stages and distant metastasis.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:南通市卫生局青年基金资助项目(编号:WQ2015021),南通市科技计划项目(编号:HS2014060)。 作者简介:赵 枰(1977-),男,硕士,副主任技师,主要从事免疫学检验工作,E-mail:zhaopinghonghong@163.com。 通讯作者:李 晗,副主任技师,E-mail:ntlh.999@163.com。
更新日期/Last Update: 1900-01-01