[1]李腾达,陈 燕,谷明莉,等.PD-L1在原发性胆汁性肝硬化患者外周血单个核细胞中的变化及意义[J].现代检验医学杂志,2017,32(05):1-3.[doi:10.3969/j.issn.1671-7414.2017.05.001]
　LI Teng-da,CHEN Yan,GU Ming-li,et al.Change of PD-L1 in Peripheral Blood Mononuclear Cells of Patients with Primary Biliary Cirrhosis and Its Significance[J].Journal of Modern Laboratory Medicine,2017,32(05):1-3.[doi:10.3969/j.issn.1671-7414.2017.05.001]
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PD-L1在原发性胆汁性肝硬化患者外周血单个核细胞中的变化及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
1-3
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Change of PD-L1 in Peripheral Blood Mononuclear Cells of Patients with Primary Biliary Cirrhosis and Its Significance
文章编号:
1671-7414(2017)05-001-03
作者:
李腾达1; 陈 燕1; 谷明莉1; 黄元兰2; 张薇薇1; 孙 懿1; 郭 杰1; 刘 云1;  徐贵霞1; 钱 琤3; 邓安梅1
1.第二军医大学长海医院,上海 200433; 2.解放军455医院,上海 200052; 3.解放军100医院,江苏苏州 215007
Author(s):
LI Teng-da1; CHEN Yan1; GU Ming-li1; HUANG Yuan-lan2; ZHANG Wei-wei1; SUN Yi1; GUO Jie1;  LIU Yun1; XU Gui-xia1; QIAN Cheng3; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China; 2.No.455 Hospital of PLA,Shanghai 200052,China; 3.No.100 Hospital of PLA,Jiangsu Suzhou 215007,China
关键词:
原发性胆汁性肝硬化;  程序性细胞死亡配体1;  肿瘤坏死因子-α;  白细胞介素-23
分类号:
R575.22; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.05.001
文献标志码:
A
摘要:
目的 探究程序性细胞死亡配体1(PD-L1)在原发性胆汁性肝硬化(PBC)患者外周血单个核细胞(PBMC)中的表达情况及意义。方法 收集2013年2月～2015年12月于长海医院确诊的23例PBC患者和23例健康对照者外周血,用密度梯度离心法分离出PBMC后进行培养,以实时荧光定量PCR法(qRT-PCR)检测PBMC中PD-L1的mRNA表达状况,以酶联免疫吸附反应(ELISA)法检测培养液中TNF-α,TGF-β,IL-23和IL-17等细胞因子的表达量,两组之间的变量比较采用两独立样本t检验,以Pearson相关系数表示两变量之间的关系。结果 实验组与对照组PD-L1的mRNA表达水平为0.23±0.08 vs 1.27±0.40(t=12.23,P<0.000 1),差异具有统计学意义。Pearson相关性分析显示PD-L1与IL-23成负相关(r=-0.531,P=0.009),差异具有统计学意义。结论 PD-L1可能参与了PBC疾病形成过程,是该病预测和治疗潜在的生物标志物。
Abstract:
Abstract:Objective To explore the expression of PD-L1 in peripheral blood mononuclear cells(PBMC)from patients with primary biliary cirrhosis(PBC)and its significance.Methods Peripheral blood of 23 healthy individuals and 23 patients with PBC diagnosed in Changhai Hospital was collected during February 2013 to December 2015.PBMC was separated by density gradient centrifugation,then cultured.The mRNA expression of PD-L1 in PBMC was examined by real-time fluorescent quantitative PCR(qRT-PCR),the expression of cytokines such as TNF-α,TGF-β,IL-23,IL-17 inmedium was examined by enzyme-linked immunosorbent assay(ELISA).Two independent samples' t test was used to compare variables in two groups.Pearson correlative coefficient was used to reveal the relationship between two variables.Results mRNA expression of PD-L1 in experiment and controlgroups was 0.23±0.08 vs 1.27±0.40(t=12.23,P<0.000 1)and there was statistical significance.Pearson correlation analysis revealed that PD-L1 was negatively correlated with IL-23(r=-0.531,P=0.009)with difference statistically.Conclusion PD-L1 might involve in the development of PBC,and could be the potential biomarker for predicting and treatingthis disease.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81671556,81601406,81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:(1990-),女,在读硕士生,主要从事感染免疫研究,E-mail: tengdali@smmu.edu.cn。 陈 燕(1977-),女,检验医师,主要从事感染免疫研究,E-mail:chenyan770628@163.com,共同第一作者。 通讯作者:邓安梅,女,教授,E-mail: amdeng70@163.com。 钱 琤,女,主治医师,E-mail: qiancheng824@126.com,共同通讯作者。
更新日期/Last Update: 1900-01-01