[1]肖鸽飞,李 桦,孟小军,等.孕妇FTO基因的表达与胰岛素水平相关性的研究[J].现代检验医学杂志,2017,32(05):44-47.[doi:10.3969/j.issn.1671-7414.2017.05.012]
　XIAO Ge-fei,LI Hua,MENG Xiao-jun,et al.Correlation Study on FTO Expression and Insulin Secretion Level in Pregnant Women[J].Journal of Modern Laboratory Medicine,2017,32(05):44-47.[doi:10.3969/j.issn.1671-7414.2017.05.012]
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孕妇FTO基因的表达与胰岛素水平相关性的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
44-47
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Correlation Study on FTO Expression and Insulin Secretion Level in Pregnant Women
文章编号:
1671-7414(2017)05-044-04
作者:
肖鸽飞; 李 桦; 孟小军; 赵艳玲; 洪琳凤; 胡玲玲
珠海市妇幼保健院检验科,广东珠海 519001
Author(s):
XIAO Ge-fei; LI Hua; MENG Xiao-jun; ZHAO Yan-ling; HONG Lin-feng; HU Ling-ling
Department of Clinical Laboratory, Zhuhai Municipal Maternal and Child Healthcare Hospital,Guangdong Zhuhai519001,China
关键词:
妊娠期糖尿病;  脂肪量和肥胖相关基因;  胰岛素
分类号:
Q786; R714.256
DOI:
10.3969/j.issn.1671-7414.2017.05.012
文献标志码:
A
摘要:
目的 探讨孕妇脂肪量和肥胖相关基因(FTO)的表达与妊娠期胰岛素水平的相关性。方法 分别选取2016年3～8月于珠海市妇幼保健院就诊的60例妊娠期糖尿病(GDM)孕妇和60例血糖正常孕妇作为研究对象,各空腹抽取2管静脉血。其中1管抗凝全血分离淋巴细胞提取RNA,并以RT-PCR法研究其淋巴细胞中FTO mRNA的表达水平; 另1管非抗凝血离心取其血清,以酶联免疫法检测血清中FTO蛋白,化学发光法检测空腹胰岛素,自动生化分析仪检测血清GLU,CHO,TG,HDL-C和LDL-C等指标的浓度,并分析其相关性。结果 病例组FTO mRNA,FTO蛋白水平、空腹血糖、OGTT 1 h和2 h血糖的结果分别为0.17%±0.10%,61.32±25.23 pg/ml,4.71±0.54 mmol/L,10.70±1.36 mmol/L和8.97±1.60 mmol/L,与对照组的0.11%±0.07%,51.47±22.97 pg/ml,4.41±0.28 mmol/L,8.05±1.04 mmol/L和6.56±0.75mmol/L相比,差异均有统计学意义(t=3.876,2.236,3.817,11.964和10.578,P均<0.05)。病例组的胰岛素水平为6.83±9.76 mU/L,与对照组的13.15±13.99 mU/L相比,差异有统计学意义(t=-2.869,P<0.05)。另外,FTO mRNA与FTO蛋白,OGTT 1 h,2 h血糖水平呈正相关,与胰岛素水平呈负相关(均r=0.232,0.292,0.242,-0.185,P<0.05),与空腹血糖和血脂水平无相关性(P>0.05)。结论GDM孕妇的FTO表达高于正常孕妇,FTO mRNA相对表达量与胰岛素呈负相关,其对血糖代谢的调节可能是通过影响胰岛素来实现。
Abstract:
Abstract:Objective To evaluate the correlation of maternal FTO expression and insulin secretion levels in pregnant women.Methods In outpatient of Zhuhai Municipal Maternal and Child HealthcareHospital,60 cases of pregnant women with gestational diabetes mellitus(GDM)and 60 cases of healthy pregnant women were recruited in this study from March 2016 to August 2016.Each pregnant woman was taken two tubes of venous blood.Lymphocytes was isolated from one tube of anticoagulated whole blood.The expressionof FTO mRNA in lymphocytes was investigated using RT-PCR.Serum was isolated from another tube of non-anticoagulant whole blood.Its FTO protein was investigated using ELISA,insulin was investigated using chemiluminescence method,and GLU,CHO,TG,HDL-C and LDL-C were investigated by automatic biochemical analyzer.The correlation of the biochemical indicators were analyzed.ResultsThe expression level of FTO mRNA,FTO protein,fasting glucose data,OGTT at 1h and 2h glucose levels in the case group were 0.17%±0.10%,61.32±25.23 pg/ml,4.71±0.54 mmol/L,10.70±1.36 mmol/L and 8.97±1.60 mmol/L respectively.The values of these biochemical indicators in control group were0.11%±0.07%,51.47±22.97 pg/ml,4.41±0.28 mmol/L,8.05±1.04 mmol/Land 6.56±0.75 mmol/L respectively.The differences were statistically significant(t=3.876,2.236,3.817,11.964 and 10.578,P<0.05).The insulin levels were 6.83±9.76 mU/L in the case group,and 13.15±13.99 mU/L incontrol group.The differences was statistically significant too(t=-2.869,P<0.05).FTO expression level was positively correlated with FTO protein,OGTT 1h glucose and OGTT 2h glucose levels(r=0.232,0.292,0.242,allP<0.05),and it was negatively correlated with insulin levels(r=-0.185,P<0.05).There was no correlation between FTO and fasting glucose expressionlevels(P>0.05).Conclusion The expression of FTO level in pregnant women with GDM was higher than that in healthy pregnant women.The relative expression of FTO mRNA was negatively correlated with insulin.And the regulation of glucose metabolism might be effected by insulin.

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备注/Memo

备注/Memo:
基金项目:珠海市医学科研基金项目(项目编号:2015J018)。 作者简介:肖鸽飞(1974-),女,硕士,主任技师,主要研究方向为遗传病的实验室诊断,E-mail:xgf8111_cn@hotmail.com。
更新日期/Last Update: 1900-01-01