[1]薛 丽,黄 芳,张彦平,等.丙肝患者血清HCV-RNA含量与肝功能及血常规指标的相关性[J].现代检验医学杂志,2017,32(05):110-114.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　XUE Li,HUANG Fang,ZHANG Yan-ping,et al.Relevance of Serum HCV-RNA Level with Routine Liver Function Indices and Blood Routine Parameters in Patients with Hepatitis C[J].Journal of Modern Laboratory Medicine,2017,32(05):110-114.[doi:10.3969/j.issn.1671-7414.2017.05.017]
点击复制

丙肝患者血清HCV-RNA含量与肝功能及血常规指标的相关性()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
110-114
栏目:
论著
出版日期:
2017-11-02

文章信息/Info

Title:
Relevance of Serum HCV-RNA Level with Routine Liver Function Indices and Blood Routine Parameters in Patients with Hepatitis C
文章编号:
1671-7414(2017)05-110-05
作者:
薛 丽1; 黄 芳1; 张彦平1; 雷 娜1; 王 妍1; 魏 伟1; 黎 明2; 李步荣1; 耿 燕1
1.西安交通大学医学院第二附属医院检验科,西安 710004; 2.西安交通大学医学院第一附属医院心血管外科,西安 710061
Author(s):
XUE Li1; HUANG Fang1; ZHANG Yan-ping1; LEI Na1; WANG Yan1; WEI wei1;  LI Ming2; LI Bu-rong1; GENG Yan1
1.Department of Clinical Laboratory, the Second Affiliated Hospital of Xi'an Jiaotong University Medical College, Xi'an 710004,China; 2.Department of Cardiovascular Surgery,the First Affiliated Hospital of Xi'an Jiaotong University Medical College,Xi'an 710061,China
关键词:
丙型肝炎;  HCV-RNA 含量;  肝功能指标;  血细胞计数参数
分类号:
R512.63; R446.112
DOI:
10.3969/j.issn.1671-7414.2017.05.017
文献标志码:
A
摘要:
目的 探讨丙肝患者血清HCV-RNA含量与肝功能和血常规指标的相关性,为丙肝患者早期诊断和治疗效果监测提供参考依据。方法 选取丙肝患者651例,应用实时荧光定量PCR法检测血清HCV-RNA含量。根据血清HCV-RNA 含量分为六组,分析患者血清HCV-RNA含量与肝功能和血常规指标的关系。结果 随着丙肝患者血清HCV-RNA含量增加,血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST),AST/ALT,谷氨酰基转移酶(GGT)和总胆红素(TBIL)均呈升高趋势,而清蛋白/球蛋白比值(A/G)呈下降趋势(ALT,AST,AST/ALT,GGT,TBIL和A/G的最低值与最高值分别是32.3±9.7 U/L与96.2±13.6 U/L,31.1±8.38 U/L与113.5±15.9U/L,0.86±0.09与1.19±0.11,29.2±14.5 U/L与52.7±16.2 U/L,17.2±4.32 μmol/L与26.0±5.58 μmol/L,0.98±0.07与1.35±0.14),差异有统计学意义(F=457.1,656.4,149.1,40.18,46.56和146.98,均P<0.01)。血细胞计数结果显示,随着血清HCV-RNA含量增加,白细胞(WBC)、中性粒细胞比值(NEUT%)、红细胞(RBC)、血红蛋白(Hb)和血小板(PLT)呈下降趋势而淋巴细胞比值(LY%)呈上升趋势[WBC,NEUT%,LY%,RBC,Hb和PLT的最低值与最高值分别是(4.30±0.22)×109/L与(6.02±0.27)×109/L,(48.13±3.56)%与(59.28±3.40)%,(31.05±3.41)%与(38.81±4.65)%,(3.73±1.70)×1012/L与(4.65±1.88)×1012/L,(122.01±5.58)g/L与(135.37±8.50)g/L,(102.65±16.87)×109/L与(148.21±14.40)×109/L],除RBC的差异无统计学意义(F=1.926,P>0.05),WBC,NEUT%,LY%,Hb和PLT的差异均具有统计学意义(F=349.0,132.145,43.65,38.91和52.21,均P<0.01)。结论 丙肝患者肝功能和血细胞计数指标与血清HCV-RNA含量有关,联合检测血清HCV-RNA含量与肝功能和血细胞计数指标对丙型肝炎的诊断及疗效观察有指导意义。
Abstract:
Abstract:Objective To explore the correlation of serum HCV-RNA level with liver function indices and blood routine parameters in patients with hepatitis C,and provide the references for the early diagnosis and monitoring the prognosis of hepatitis C.Methods The study population comprised 651 patients who were diagnosed with hepatitis C in the Second Affiliated Hospital of Medical College of Xi'an Jiaotong University.Serum level of HCV RNA was determined by quantitative real-time polymerase chainreaction(real-time PCR).The data obtained were divided into six groups according to the result of HCV RNA level.Then the correlation of serum HCV-RNA levelwith liver function indices and blood routine parameters was analyzed.Results With the rising level of serum HCV-RNA,the levels of liver function indices including ALT,AST,AST/ALT,GGT and TBIL were all significant increased in hepatitis C patients.However,A/G presented a declining trendwith the increase of serum HCV-RNA level.The lowest value and highest value ofALT,AST,AST/ALT,GGT,TBIL and A/G were 32.3±9.7 U/L and 96.2±13.6 U/L,31.1±8.38 U/L and 113.5±15.9 U/L,0.86±0.09 and 1.19±0.11,29.2±14.5 U/L and 52.7±16.2 U/L,17.2±4.32 μmol/L and 26.0±5.58 μmol/L,0.98±0.07 and 1.35±0.14,respectively.Significant differences in ALT,AST,AST/ALT,GGT,TBIL and A/G were observed among these groups(F value was 457.1,656.4,149.1,40.18,46.56 and 146.98,respectively.all P<0.01).Moreover,the levels of blood routine parameters including WBC,NEUT%,RBC,Hb and PLT were all significant decreased while that of LY% was increased with the rising level of serum HCV-RNA in hepatitis C patients.The lowest value andhighest value of WBC,NEUT%,LY%,RBC,Hb and PLT were(4.30±0.22)×109/Land(6.02±0.27)×109/L,(48.13±3.56)% and(59.28±3.40)%,(31.05±3.41)% and(38.81±4.65)%,(3.73±1.70)×1012/L and(4.65±1.88)×1012/L,(122.01±5.58)g/L and(135.37±8.50)g/L,(102.65±16.87)×109/L and(148.21±14.40)×109/L,respectively.There was no significant difference in RBC among groups(F=1.926,all P<0.05).However,the differences in other blood routine parameters than RBC among groups were statistically significant(F value of WBC,NEUT%,LY%,Hb and PLT was 349.0,132.145,43.65,38.91 and 52.21,respectively.all P<0.01).ConclusionSerum HCV-RNA level was correlated with routine liver function indices and blood routine parameters.It is important to perform the combined detection of serum HCV-RNA level,routine liver function indices and blood routine parameters in hepatitis C patients,which can help to guide diagnosis and to evaluate the prognosis.

参考文献/References:

[1] Mohd Hanafiah K,Groeger J,Flaxman AD,et al.Glo-bal epidemiology of hepatitis C virus infection:new estimates of age-specific antibody to HCV sero-prevalence[J].Hepatology,2013,57(4):1333-1342.
[2] Rao HY,Wei L,Lopez-Talavera JC,et al.Distribution and clinical correlates of viral and host genotypes in Chinese patients with chronic hepatitis C virus infection[J].J Gastroenterol Hepatol,2014,29(3):545-553.
[3] Thrift AP,El-Serag HB,Kanwal F.Global epidemiology and burden of HCVinfection and HCV-related disease[J].Nat Rev Gastroenterol Hepatol,2017,14(2):122-132.
[4] 方超萍,张薇薇,孙小波,等.丙型肝炎患者不同病毒载量与病毒学应答及肝功能改变的相关性[J].现代检验医学杂志,2012,27(5):18-21. Fang CP,Zhang WW,Sun XB,et al.Correlation between the serum level of HCV-RNA,virological response and the change of liver function in patientswith hepatitis C[J].J Mod Lab Med,2012,27(5):18-21.
[5] 中华医学会肝病学分会,中华医学会感染病学分会.丙型肝炎防治指南(2015更新版)[J].中华肝脏病杂志,2015,23(12):933-949. Chinese Society of Hepatology,and Chinese Society of Infectious Diseases,Chinese Medical Association.The guideline of prevention and treatment for hepatitis C:a 2015 update[J].Chinese Journal of Hepatology,2015,23(12):933-949.
[6] 杨 杰,崔 敬,刘 春,等.丙型肝炎核心抗原检测在丙型肝炎诊断中的意义[J].临床肝胆病杂志,2013,29(2):128-131. Yang J,Cui J,Liu C,et al.Detection of HCV core antigen and its diagnostic significance[J].Journal of Clinical Hepatology,2013,29(2):128-131.
[7] 甘志超,刘 莹,赵冰红,等.丙型肝炎患者自身抗体检出率、丙肝病毒载量及肝功能间的相关性[J].现代检验医学杂志,2016,31(3):108-110,113. Gan ZC,Liu Y,Zhao BH,et al.Correlation among the positive rate of autoantibody,HCV load and the status of liver function in patients infected HCV[J].J Mod Lab Med,2016,31(3):108-110,113.
[8] Shahid M,Idrees M,Nasir B,et al.Correlation of biochemical markers and HCV RNA titers with fibrosis stages and grades in chronic HCV-3a patients[J].Eur J Gastroenterol Hepatol,2014,26(7):788-794.
[9] 张洪美,袁美莲.丙型肝炎患者血清自身抗体和 AST/ALT在诊断丙肝后肝硬化中的临床意义[J].中国实验诊断学,2016,20(3):443-445. Zhang HM,Yuan ML.The clinical significance of serum autoantibodiesand AST/ALT in diagnosis of cirrhosis following chronic hepatitis C[J].Chinese Journal of Laboratory Diagnosis,2016,20(3):443-445.
[10] 张育锋,黄东平,黄炳坤.HCV-RNA及ALT,AST联合检测在丙肝患者诊断治疗中的应用研究[J].临床医学工程,2015,22(7):923-924,927. Zhang YF,Huang DP,Huang BK.Application research of the combined detection of HCV-RNA and ALT/AST in patients with hepatitis C[J].Clinical Medical & Engineering,2015,22(7):923-924,927.
[11] 武抗抗,杨晨辰,黄 睿,等.慢性乙型肝炎患者不同免疫状态下血清γ谷氨酰转肽酶水平变化及其临床意义[J].实用医学杂志,2014,30(13):2068-2071. Wu KK,Yang CC,Huang R,et al.Expression and clinical significance of serum γ-glutamyltransferase in patients with chronic hepatitis B virus infectionin different immune status[J].Journal of Practical Medicine,2014,30(13):2068-2071.
[12] 马志慧.病毒性肝炎常见的化验指标及其临床意义分析[J].中国医药指南,2013,11(14):627-628. Ma ZH.Viral hepatitis common laboratory indexes and their clinical significance[J].Guide of China Medicine,2013,11(14):627-628.
[13] Giannini E.Review article:thrombocytopenia in ch-ronic liver disease and pharmacologic treatment options[J].Aliment Pharmacol Ther,2006,23(8):1055-1065.

相似文献/References:

[1]黄启强,黄丽庆,周青,等.探讨CD4+CD25+Foxp3+调节性T淋巴细胞在HCV早期感染的作用[J].现代检验医学杂志,2015,30(06):64.[doi:10.3969/j.issn.1671-7414.2015.06.018]
　HUANG Qi-qiang,HUANG Li-qing,ZHOU Qing,et al.Study or Role of the CD4+CD25+Foxp3+ Regulatory T Lymphocytes in the Early Infection of HCV[J].Journal of Modern Laboratory Medicine,2015,30(05):64.[doi:10.3969/j.issn.1671-7414.2015.06.018]
[2]王楷翔a,曹　明a,陈　晖b,等.HCV感染患者血清miR-199和miR-483水平表达及其临床价值[J].现代检验医学杂志,2021,36(05):58.[doi:10.3969/j.issn.1671-7414.2021.05.013]
　WANG Kai-xianga,CAO Minga,CHEN Huib,et al.Expression and Clinical Value of Serum miR-199 and miR-483 inPatients with HCV Infection[J].Journal of Modern Laboratory Medicine,2021,36(05):58.[doi:10.3969/j.issn.1671-7414.2021.05.013]
[3]武 倩,李 英,马燕粉,等.丙型肝炎肝硬化患者进展为肝细胞肝癌预测模型建立及验证[J].现代检验医学杂志,2024,39(05):6.[doi:10.3969/j.issn.1671-7414.2024.05.002]
　WU Qian,LI Ying,MA Yanfen,et al.Establishment and Validation of Prediction Model for Hepatocellular Carcinoma Progression in Patients with Hepatitis C Cirrhosis[J].Journal of Modern Laboratory Medicine,2024,39(05):6.[doi:10.3969/j.issn.1671-7414.2024.05.002]

备注/Memo

备注/Memo:
基金项目:中央高校基本科研业务经费(No.xjj2016109)。 作者简介:薛 丽,女,博士,主管技师,主要从事分子生物学检验工作,E-mail:xuelially@163.com。 通讯作者:耿 燕,E-mail:gengyanjyk@126.com。
更新日期/Last Update: 1900-01-01