[1]崔福义,杨 维.-20℃保存血清标本是否混匀对临床常规生化项目检验结果的影响观察[J].现代检验医学杂志,2017,32(05):126-129.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　CUI Fu-yi,YANG Wei.Observation of Whether to Mix Evenly the Serum Samples Preserved at -20℃ for Clinical Routine Biochemical Test Results[J].Journal of Modern Laboratory Medicine,2017,32(05):126-129.[doi:10.3969/j.issn.1671-7414.2017.05.017]
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-20℃保存血清标本是否混匀对临床常规生化项目检验结果的影响观察()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年05期
页码:
126-129
栏目:
研究简报·实验技术
出版日期:
2017-11-02

文章信息/Info

Title:
Observation of Whether to Mix Evenly the Serum Samples Preserved at -20℃ for Clinical Routine Biochemical Test Results
文章编号:
1671-7414(2017)05-126-04
作者:
崔福义1; 杨 维2
1.唐山市丰润区人民医院检验科,河北唐山 064000; 2.西安市中医医院检验科,西安 710021
Author(s):
CUI Fu-yi1; YANG Wei2
1.Department of Clinical Laboratory, Fengrun District People's Hospital of Tangshan,Hebei Tangshan 064000,China; 2.Department of Clinical Laboratory,Xi'an Traditional Chinese Medicine Hospital,Xi'an 710021,China
关键词:
血清标本;  温度;  生化结果;  混匀;  保存条件
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2017.05.017
文献标志码:
A
摘要:
目的 探讨血清标本在-20℃保存下生化检验结果变化及混匀对其影响。方法 收集的24例患者的新鲜血清进行常规生化检测后,分别置于四组编号相同容量为1 ml的样本管中,一组25℃保存24 h,一组4℃冰箱保存24 h,两组置于-20℃保存24 h(一组未混匀检测,一组混匀后检测),并将四组血清样本的生化结果与初始结果进行比较。结果 25℃室温保存24 h后,ALT,AST,TBIL和DBIL四项生化指标的平均水平均低于初始结果(t=2.130,2.308,2.134,3.286,P=0.042,0.029,0.042,0.003),其余生化指标结果比较,差异均无统计学意义(P>0.05); 4℃冰箱保存24 h后,TBIL,DBIL的水平低于初始水平(t=2.103,2.089,P=0.045,0.046),其余生化指标比较差异均无统计学意义(P>0.05); -20℃冰箱保存24 h的两组血清样本中,未进行混匀的样本血清生化结果与初始结果比较,差异均有统计学意义(t=-4.218～11.710,均P<0.05),而混匀的血清样本的生化结果与初始结果比较,差异均无统计学意义(P>0.05)。结论 血清样本化学成分的均一性在4℃冰箱中不会有所改变,可将不能及时检测的血清样本置于其中保存,而-20℃冰箱保存的血清样本的上下层化学成分浓度差异显著,上高下低,而检测前进行充分混匀可保证其生化检测结果的准确性。
Abstract:
Abstract:Objective To explore the change of conventionalbiochemical test results of serum specimens saved -20℃ and blending of its effects.Methods The fresh serum of 24 patients were conducted routine biochemical tests,respectively in four groups of numbers in the same capacity of 1 ml of the sample tube,a set of 25℃ kept 24 hours a day,a group of 4 ℃ refrigerated for 24 hours,24 hours at -20℃ save the two groups(a group was not incorporated,a set of blended),and the four groups of serum samples in biochemical results comparing with the initial results.Results 25℃ at room temperature preservation after 24 hours,ALT,AST,TBIL and DBIL biochemical indicators of four average were below the initial results(t=2.130,2.308,2.130 and 2.308,P=0.042,0.029,0.042 and 0.003),there were no statistically significant difference between the rest of the biochemical index results comparison(P>0.05).4℃ refrigerator after 24 hours,TBIL,DBIL levels below the initial level(t=2.103 and 2.089,P=0.045 and 0.046),and the rest of the biochemical indicators to compare differences had no statistical significance(P>0.05).-20℃ refrigerated in the two groups of samples,not each sample of serum biochemical results compared with the initial results,were kept statistically significant difference(t=-4.218～11.710,all P<0.05),while serum samples of 15 items and biochemical indexes of blending the results compared with the initial results,the average level of had no statistical significance(P>0.05).Conclusion Serum samples of the chemical composition of the homogeneity in the 4℃ refrigerator will not change,but will not be able to timely detection of serum samples in the preservation,and minus -20 ℃ refrigerator on the serum samples of a significant difference was found in the lower concentration of chemical components,compete on low,and tests conducted before fully blending can ensure the precision of its biochemical test results.

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备注/Memo

备注/Memo:
作者简介:崔福义(1963-),男,主管检验师,E-mail:630452329@qq.com。 通讯作者:杨 维(1981-),女,硕士,主管检验师,研究方向:临床血液学检验,E-mail:95250473@qq.com。
更新日期/Last Update: 1900-01-01