[1]王晓囡,陆士奇,段欠欠,等.6种常见脑炎病毒多重PCR检测体系的建立和应用[J].现代检验医学杂志,2017,32(06):11-14，18.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　WANG Xiao-nan,LU Shi-qi,DUAN Qian-qian,et al.Development of A Multiplex PCR Method for Detecting Six Types of Viruses Causing Encephalitis[J].Journal of Modern Laboratory Medicine,2017,32(06):11-14，18.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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6种常见脑炎病毒多重PCR检测体系的建立和应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
11-14，18
栏目:
论著
出版日期:
2017-12-25

文章信息/Info

Title:
Development of A Multiplex PCR Method for Detecting Six Types of Viruses Causing Encephalitis
文章编号:
1671-7414(2017)06-011-05
作者:
王晓囡1; 陆士奇2; 段欠欠1; 季建刚3; 张丽君3; 刘婉婉1; 奚邦生1; 孙万平1
1.苏州大学药学院药理学系,江苏苏州 215021; 2.苏州大学附属第一医院急诊科, 江苏苏州 215006; 3.苏州高新区卫生监督所,江苏苏州 215010
Author(s):
WANG Xiao-nan1; LU Shi-qi2; DUAN Qian-qian1; JI Jian-gang3; ZHANG Li-jun3;  LIU Wan-wan1; XI Bang-sheng1; SUN Wan-ping1
1.College of Pharmaceutical Sciences, Soochow University,Jiangsu Suzhou 215021,China; 2.Department of Emergency, the First Affiliated Hospital of Soochow University,Jiangsu Suzhou 215006,China; 3.Health Surveillance Institute of Suzhou Hi-Tech Development Zone,Jiangsu Suzhou 215010,China
关键词:
多重聚合酶链式反应;  病毒性脑炎;  核酸检测
分类号:
Q503; R373
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 建立单纯疱疹病毒1(HSVⅠ),2型(HSVⅡ)、水痘带状疱疹病毒(VZV)、EB病毒(EBV)、肠道病毒71型(EV71)及巨细胞病毒(CMV)的多重聚合酶链式反应(mPCR)体系。方法 对HSVⅠ,HSVⅡ,VZV,EBV,EV71及CMV设计特异引物,建立mPCR检测方法,并进行敏感性验证。收集2014～2015年在苏州大学附属第一医院收治的15例临床疑似病毒性脑炎(VE)患者脑脊液(CSF)标本,采用mPCR体系进行核酸检测。结果 6对引物特异性扩增六种病毒,构建的mPCR体系敏感度超过103 copies/μl; 该mPCR体系对15例临床脑脊液标本进行检测,共6例阳性标本(6/15,40%),其中HSVⅠ5例,CMV 1例。结论 初步建立了一种能够同时筛查6种常见脑炎病毒的mPCR方法。
Abstract:
Abstract:Objective To develop a new mPCR method for rapid diagnosis of six types of encephalitis causing viruses of HSVI,HSVII,VZV,EBV,EV71 and CMV.Methods Six pairs of specific primers for CMV,EV71,HSV I,VZV,EBV and HSV II were designed.The mPCR detection methodwas established and the sensitivity was detected.In order to verify the clinical application value of their multiplex PCR system,fifteen cerebrospinal fluidspecimens of clinically suspected VE from the First Affiliated Hospital of Soochow University from 2014 to 2015 were examined by the mPCR method.Results The 6 pairs of primers did not interfere with each other,and the sensitivity of the mPCR system was over 103 copies/μl.Among 15 cerebrospinal fluid specimens from patients with suspected viral encephalitis,six specimens(6/15,40%)were tested positive by the mPCR.Among them,HSV I was 5 and CMV was 1.Conclusion The mPCR method for detecting sixtypes of encephalitis-associated virus at same time was established with highspecificity,sensitivity and stability.

参考文献/References:

[1] Bautista C.Central nervous system infections[J].Crit Care Nurs Clin North Am,2013,25(3):11-12.
[2] 高 珺,王 琼,林广裕,等.病毒性脑炎患儿脑脊液人类鼻病毒检测的价值[J].现代检验医学杂志,2015,30(2):11-15. Gao J,Wang Q,Lin GY,et al.Value of human rhinovirus detected incerebrospinal fluid of children with viral encephalitis[J].J Mob Lab Med,2015,30(2):11-15.
[3] Parisi SG,Basso M,Del Vecchio C,et al.Viral infections of the centralnervous system in elderly patients:a retrospective study[J].International Journal of Infectious Diseases,2016,44(C):8-10.
[4] 王云龙,李智涛,李玉林,等.荧光定量RT-PCR检测临床标本中的肠道病毒RNA[J].中国卫生检验杂志,2009,19(6):1329-1331,1346. Wang YL,Li ZT,Li YL,et al.Enterovirus RNA detection in clinical samples by real-time RT-PCR[J].Chinese Journal of Health LaboratoryTechnology,2009,19(6):1329-1331,1346.
[5] Hinson VK,Tyor WR.Update on viral encephalitis[J].Curr Opin Neurol,2001,14(3):369-374.
[6] 孙卫国,孙雯娜,侯江厚,等.人巨细胞病毒pp150-gp52蛋白原核可溶性表达与IgM捕获ELISA方法建立和应用[J].现代检验医学杂志,2015,30(5):1-4. Sun WG,Sun WN,Hou JH,et al.Soluble expression of HCMV pp 150-gp52 protein in prokaryotic system and establishment of IgM capture-ELISA[J].J Mob Lab Med,2015,30(5):1-4.
[7] 杨吉星,居丽雯,施 强,等.上海地区水痘带状疱疹病毒基因型研究[J].中国卫生检验杂志,2008,18(2):223-224. Yang JX,Ju LW,Shi Q,et al.Research of genotype of varicella-zoster virus in Shanghai[J].Chinese Journal of Health Laboratory Technology,2008,18(2):223-224.
[8] 曾昭森.荧光RT-PCR同步检测肠道病毒71型和柯萨奇病毒A组16型方法的建立及应用[J].临床和实验医学杂志,2012,11(16):1327-1328. Zeng ZS.Detection of a fluorescent RT-PCR method for detecting Enterovirus 71 and coxsackie A16[J].Journal of Clinical and Experimental Medicine,2012,11(16):1327-1328.
[9] 郭建巍,高毓蕊,田占月,等.EB病毒和人巨细胞病毒核酸检测的实验研究[J].现代检验医学杂志,2014,29(1):105-107. Guo JW,Gao YR,Tian ZY,et al.Experiment research for detecting DNA of epstein-barr virus and human cytomegalovirus[J].J Mob Lab Med,2014,29(1):105-107.
[10] Ziyaeyan M,Alborzi A,Borhani Haghighi A,et al.Diagnosis andquantitative detection of HSV DNA in samples from patients with suspected herpes simplexencephalitis[J].Brazilian Journal of Infectious Diseases,2011,15(3):211-214.
[11] Cho HK,Lee NY,Lee H,et al.Enterovirus 71-associated hand,foot andmouth diseases with neurologic symptoms,a university hospital experience in Korea,2009[J].Korean Journal of Pediatrics,2010,53(5):639-643.
[12] Glaser CA,Honarmand S,Anderson LJ,et al.Beyond viruses:clinical profiles and etiologies associated with encephalitis[J].Clin Infect Dis,2006,43(12):1565-1577.
[13] 陈 勇,吴华平.小儿病毒性脑炎的诊断与治疗[J].实用儿科临床杂志,2012,27(24):1863-1865. Chen Y,Wu HP.Diagnosis and treatment of viral encephafitis in children[J].J Appl Clin Pediatr,2012,27(24):1863-1865.

相似文献/References:

[1]高 珺,王 琼,林广裕,等.病毒性脑炎患儿脑脊液人类鼻病毒检测的价值[J].现代检验医学杂志,2015,30(02):11.[doi:10.3969/j.issn.1671-7414.2015.02.004]
　GAO Jun,WANG Qiong,LIN Guang-yu,et al.Value of Human Rhinovirus Detected in Cerebrospinal Fluid of Children with Viral Encephalitis[J].Journal of Modern Laboratory Medicine,2015,30(06):11.[doi:10.3969/j.issn.1671-7414.2015.02.004]
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备注/Memo

备注/Memo:
基金项目:苏州市重大疾病、传染病预防和控制关键技术(研究)项目(Gwzx201606),苏州市民生项目(SS201660,SS201511)。 作者简介:王晓囡(1992-),女,硕士在读,专业:药理学,主要研究方向:分子诊断,E-mail:1173588898@qq.com。 通讯作者:孙万平,男,博士,副教授,主要研究方向:分子诊断,E-mail:sunwanping@suda.edu.cn。
更新日期/Last Update: 2017-12-26