[1]李腾达,刘 鹏,龙曙萍,等.隐球菌脑膜炎患者血清外泌体CD93表达增高及意义[J].现代检验医学杂志,2017,32(06):23-25.[doi:10.3969/j.issn.1671-7414.2017.06.001]
 LI Teng-da,LIU Peng,LONG Shu-ping,et al.Elevated Expression of CD93 in Serous Exosomes from Patients with Cryptococcal Meningitis and Its Significance[J].Journal of Modern Laboratory Medicine,2017,32(06):23-25.[doi:10.3969/j.issn.1671-7414.2017.06.001]
点击复制

隐球菌脑膜炎患者血清外泌体CD93表达增高及意义()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
23-25
栏目:
论著
出版日期:
2017-12-25

文章信息/Info

Title:
Elevated Expression of CD93 in Serous Exosomes from Patients with Cryptococcal Meningitis and Its Significance
文章编号:
1671-7414(2017)06-023-03
作者:
李腾达1刘 鹏1龙曙萍1刘 云1黄元兰2张薇薇1郭 杰1谷明莉1邓安梅1
1.第二军医大学长海医院,上海 200433; 2.解放军455医院,上海 200052
Author(s):
LI Teng-da1LIU Peng1LONG Shu-ping1LIU Yun1HUANG Yuan-lan2ZHANG Wei-wei1 GUO Jie1GU Ming-li1DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China; 2.No.455 Hospital of PLA,Shanghai 200052,China
关键词:
隐球菌脑膜炎 外泌体 CD93 白介素-1β 白介素-17
分类号:
R519.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 检测隐球菌脑膜炎患者血清中外泌体表达CD93的水平,进一步探究其临床意义。方法 实验组为2012年11月~2016年12月在上海长海医院和长征医院确诊的38例隐球菌脑膜炎患者的血清,诊断标准为脑脊液染色新生隐球菌阳性或培养阳性; 对照组为同期收集的38例健康人血清。血清外泌体的分离采用超高速离心法; 外泌体表面抗原的鉴定采用磁珠捕获结合流式细胞仪检测法; 血清中细胞因子用ELISA法进行蛋白定量。实验组与对照组检测值的比较采用两独立样本的t检验,两变量之间的相关性以Pearson系数表示。结果 流式结果表明,实验组血清外泌体中CD93的表达水平高于对照组为(79.11±19.31 vs 23.98±6.56)%,差异具有统计学意义(t=16.66,P<0.000 1)。IFN-γ在实验组与对照组血清中的表达水平为(39.78±10.77 vs 58.98±16.99)pg/ml,差异具有统计学意义(t=5.884,P<0.000 1); IL-17在实验组与对照组血清中的表达水平为(16.32±4.03 vs 3.11±0.87)pg/ml,差异具有统计学意义(t=19.75,P<0.000 1); IL-1β在实验组与对照组血清中的表达水平为(57.12±12.98 vs 13.45±4.78)pg/ml,差异具有统计学意义(t=19.46,P<0.000 1)。实验组血清中CD93+exosomes所占的百分比与IFN-γ,IL-17,IL-1β的蛋白表达量呈正相关(r=0.488,0.456,0.532,均P<0.01)。结论 隐球菌脑膜炎疾病过程中,CD93在外泌体的表达可能通过影响CD4+T细胞参与了该病病程。
Abstract:
Abstract:Objective To examine the expression of CD93 inserous exosomes from patients with cryptococcal meningitis,further explore its clinical significance.Methods The 38 experimental serum samples were from patients who received the diagnosis with cryptococcal meningitis in Changhai Hospital and Changzheng Hospital in Shanghai from November 2012to December 2016.The diagnosis standardization was that the cerebrospinal fluid dyeing was positive or the culturing was positive.The 38 controls were collected from the health individuals examined at the same time.The exosomes in serumwas separated by the ultracentrifuge method.Magnetic bead-capture combined with the flow cytometry method was used to identify antigens on the surface of exosomes.The protein level of cytokines in serum was quantified by ELISA method.The comparison of tested values from experimental and controlled groups was measured by two independent samples' t test,and the correlation between two variates was showed by Pearson coefficient.Results The resultsof flow cytometry showed that the expression of CD93 on exosomes in the serum samples from experimental group was higher than that in the controlled group,which was(79.11±19.31 vs 23.98±6.56)%,with difference in statistics(t=16.66,P<0.000 1).The expression levels of IFN-γ in serum samplefrom experimental and control groups were(39.78±10.77 vs 58.98±16.99)pg/ml,withdifference in statistics(t=5.884,P<0.000 1).The expression levels of IL-17 in serum sample from experimental and control groups were(16.32±4.03 vs 3.11±0.87)pg/ml,with difference in statistics(t=19.75,P<0.000 1).The expression levels of IL-1β in serum sample from experimental and control groups were(57.12±12.98 vs 13.45±4.78)pg/ml,with difference in statistics(t=19.46,P<0.000 1).The percentage of CD93+exosomes was positively correlative with IFN-γ,IL-17,IL-1β(r=0.488,0.456,0.532,P<0.01)in serum samples from the experimental group and there was statistical difference.Conclusion In the disease process of cryptococcalmeningitis,the expression of CD93 on exosomes might involve in the diseases'processes by affecting the CD4+T cells.

参考文献/References:

[1] Srikanta D,Santiago-Tirado FH,Doering TL.Cryptococcus neoformans:historical curiosity to modern pathogen[J].Yeast,2014,31(2):47-60.
[2] Panackal AA, Wuest SC, Lin YC,et al.Paradoxical immune responses in Non-HIV cryptococcal meningitis[J].PLoS Pathogens,2015,11(5):e1004884.
[3] Rohatgi S,Pirofski LA.Host immunity to Cryptococcus neoformans[J].Future Microbiology,2015,10(4):565-581.
[4] Record M,Carayon K,Poirot M,et al.Exosomes as new vesicular lipid transporters involved in cell-cell communication and various pathophysiologies[J].Biochimicaet Biophysica Acta,2014,1841(1):108-120.
[5] Yang C,Chalasani G,Ng YH,et al.Exosomes rele-ased from Mycoplasma infected tumor cells activate inhibitory B cells[J].PLoS One,2012,7(4):e36138.
[6] Liu C,Cui Z,Wang S,et al.CD93 and GIPC expression and localization during central nervous system inflammation[J].Neural Regeneration Research,2014,9(22):1995-2001.
[7] Greenlee-Wacker MC,Galvan MD,Bohlson SS.CD9 3:recent advances and implications in disease[J].Current Drug Targets,2012,13(3):411-420.
[8] 李腾达,龙曙萍,徐贵霞,等.CD26/DPP4对隐球菌脑膜炎患者CD4+T细胞及相关细胞因子的影响与临床意义[J].现代检验医学杂志,2016,31(5):38-41. Li TD,Long SP,Xu GX,et al.Affection of CD26/DPP4 on CD4+T cellsand relative cytokines in patients with crytococcal meningitis and its clinicalsignificance[J].J Mod Lab Med,2016,31(5):38-41.
[9] Hosseini HM, Fooladi AA, Nourani MR,et al.The role of exosomes in infectious diseases[J].Inflammation & Allergy Drug Targets,2013,12(1):29-37.
[10] Melo SA,Luecke LB,Kahlert C,et al.Glypican-1 identifies cancer exosomes and detects early pancreatic cancer[J].Nature,2015,523(7559):177-182.
[11] Tran TH,Mattheolabakis G,Aldawsari H,et al.Exosomes as nanocarriersfor immunotherapy of cancer and inflammatory diseases[J].Clinical ImmunoLogy,2015,160(1):46-58.
[12] Yanaba K,Asano Y,Noda S,et al.Augmented production of soluble CD93 in patients with systemic sclerosis and clinical association with severity of skin sclerosis[J].The British Journal of Dermatology,2012,167(3):542-547.

相似文献/References:

[1]张 蕾a,刘挺挺a,叶 辛b,等.IL-33在隐球菌脑膜炎患者外周血单个核中的表达及临床意义[J].现代检验医学杂志,2016,31(02):29.[doi:10.3969/j.issn.1671-7414.2016.02.009]
 ZHANG Leia,LIU Ting-Tinga,YE Xinb,et al.Expression of IL-33 in Patients with Cryptococcal Meningitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2016,31(06):29.[doi:10.3969/j.issn.1671-7414.2016.02.009]
[2]刘挺挺,谷明莉,张 蕾,等.隐球菌脑膜炎患者外周血CD200表达水平及其临床意义[J].现代检验医学杂志,2016,31(02):35.[doi:10.3969/j.issn.1671-7414.2016.02.011]
 LIU Ting-ting,GU Meng-li,ZHANG Lei,et al.Expression Level and Clinical Significance of CD200 in Peripheral Blood for Patients with Cryptococcal Meningitis[J].Journal of Modern Laboratory Medicine,2016,31(06):35.[doi:10.3969/j.issn.1671-7414.2016.02.011]
[3]胡红丽a,杨静文a,谷明莉b,等.MIF在隐球菌脑膜炎患者外周血单个核细胞中升高及临床意义[J].现代检验医学杂志,2017,32(01):16.[doi:10.3969/j.issn.1671-7414.2017.01.005]
 HU Hong-lia,YANG Jing-wena,GU Ming-lib,et al.Increased Expression of MIF in Peripheral Blood Mononuclear Cells from Patients with Crytococcal Meningitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(06):16.[doi:10.3969/j.issn.1671-7414.2017.01.005]
[4]李腾达,黄元兰,龙曙萍,等.CD36在隐球菌脑膜炎患者外周血单个核细胞中表达升高及其临床意义[J].现代检验医学杂志,2018,33(01):56.[doi:10.3969/j.issn.1671-7414.2018.01.001]
 LI Teng-da,HUANG Yuan-lan,LONG Shu-ping,et al.High Expression of CD36 in Peripheral Blood Mononuclear Cells from Patients with Crytococcal Meningitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2018,33(06):56.[doi:10.3969/j.issn.1671-7414.2018.01.001]
[5]李腾达,刘 鹏,龙曙萍,等.表面增强拉曼光谱技术检测隐球菌脑膜炎患者血清中CD93+外泌体及其意义[J].现代检验医学杂志,2018,33(04):4.[doi:10.3969/j.issn.1671-7414.2018.04.002]
 LI Teng-da,LIU Peng,LONG Shu-ping,et al.Detection of Serum CD93+Exosomes in Patients with Cryptococcal Meningitis by Surface-Enhanced Raman Scattering Technology and Its Significance[J].Journal of Modern Laboratory Medicine,2018,33(06):4.[doi:10.3969/j.issn.1671-7414.2018.04.002]
[6]李腾达,龙曙萍,黄元兰,等.自身免疫性胰腺炎患者血清外泌体中CD69表达升高及意义[J].现代检验医学杂志,2018,33(02):8.[doi:10.3969/j.issn.1671-7414.2018.02.001]
 LI Teng-da,LONG Shu-ping,HUANG Yuan-lan,et al.Elevated Expression of CD69 in Serum Exosomes from Patients with Autoimmune Pancreatitis and Its Significance[J].Journal of Modern Laboratory Medicine,2018,33(06):8.[doi:10.3969/j.issn.1671-7414.2018.02.001]
[7]李腾达,刘 鹏,龙曙萍,等.隐球菌脑膜炎患者外周血单个核细胞PILRα升高及意义[J].现代检验医学杂志,2018,33(03):11.[doi:10.3969/j.issn.1671-7414.2018.03.004]
 LI Teng-da,LIU Peng,LONG Shu-ping,et al.Increasing Expression of PILRα in Peripheral Blood Mononuclear Cells from Patients with Cryptococcal Meningitis and Its Significance[J].Journal of Modern Laboratory Medicine,2018,33(06):11.[doi:10.3969/j.issn.1671-7414.2018.03.004]
[8]范维肖,刁艳君,马越云,等.超速离心法与QIAGEN膜亲和柱法提取前列腺癌细胞培养上清外泌体的方法学比较[J].现代检验医学杂志,2019,34(03):6.[doi:10.3969/j.issn.1671-7414.2019.03.002]
 FAN Wei-xiao,DIAO Yan-jun,MA Yue-yun,et al.Comparison of Ultracentrifugation and Membrane Based-Affinity ColumnMethods in Exosome Isolation from Supernatants of Prostate Cancer Cells[J].Journal of Modern Laboratory Medicine,2019,34(06):6.[doi:10.3969/j.issn.1671-7414.2019.03.002]

备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81671556,81601406,81471605,81501397,31500721,81501398,81401358,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:李腾达(1990-),女,在读硕士研究生,主要从事自身免疫研究,E-mail:tengdali@smmu.edu.cn。 刘 鹏(1982-),女,在读博士研究生,主要从事自身免疫研究,E-mail:ppliu23@163.com,共同第一作者。 通讯作者:邓安梅,女,教授,E-mail:amdeng70@163.com。 谷明莉,女,检验技师,E-mail:mingligu@126.com,共同通讯作者。
更新日期/Last Update: 2017-12-26