[1]刘家玲,袁 军,胡方芳.贵州地区孕晚期妇女生殖道无乳链球菌定植筛查及CAMP试验阴性的GBS菌株基因测序分析研究[J].现代检验医学杂志,2018,33(01):14-18.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　LIU Jia-ling,YUAN Jun,HU Fang-fang.Screening of Streptococcus Agalactiae of Genitourinary Tractin the Late Pregnant Women in Guizhou Province and the Genetic Sequencing Analysis[J].Journal of Modern Laboratory Medicine,2018,33(01):14-18.[doi:10.3969/j.issn.1671-7414.2018.01.001]
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贵州地区孕晚期妇女生殖道无乳链球菌定植筛查及CAMP试验阴性的GBS菌株基因测序分析研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
14-18
栏目:
论著
出版日期:
2018-02-05

文章信息/Info

Title:
Screening of Streptococcus Agalactiae of Genitourinary Tractin the Late Pregnant Women in Guizhou Province and the Genetic Sequencing Analysis
文章编号:
1671-7414(2018)01-014-05
作者:
刘家玲1; 袁 军2; 胡方芳3
1.遵义医学院,贵州遵义 563000; 2.贵阳市第二人民医院,贵阳 550001; 3.贵州省人民医院,贵阳 550001
Author(s):
LIU Jia-ling1; YUAN Jun2; HU Fang-fang3
1.Zunyi Medical University, Guizhou Zunyi 563000,China; 2.Guizhou Second People's Hospital,Guiyang550001, China; 3.the People's Hospital of Guizhou Province,Guiyang 550001,China
关键词:
无乳链球菌;  CAMP阴性;  cfb基因;  耐药性
分类号:
R378.12; Q781
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 了解贵州地区孕晚期妇女泌尿生殖道无乳链球菌(GBS)定植状况及CAMP阴性菌株的鉴定分析。方法 收集2016年5月～2017年5月贵州省人民医院产科门诊孕晚期妇女生殖道分泌物进行细菌培养、分离鉴定及药敏试验; 通过生化试验及16S rDNA序列分析的方法对CAMP试验阴性的GBS菌株进行种属鉴定并检测其cfb基因。结果 3 794例样本共分离出118株无乳链球菌,阳性率为3.11%; 其中1株呈β-溶血且cfb基因检测阳性,但CAMP试验阴性,经16S rDNA序列分析确认为无乳链球菌; 药敏结果统计未发现GBS对青霉素、阿莫西林、利奈唑胺、万古霉素、头孢噻肟、头孢吡肟耐药的菌株,耐药率较高的抗生素依次为红霉素(81.3%)、克林霉素(62.7%)、左氧氟沙星(72.9%)。结论 CAMP试验阴性的无乳链球菌非常少见,3 794例样本中仅分离到了一株,因此临床实验室应警惕CAMP试验阴性的无乳链球菌,避免漏检,为临床提供更为准确的检测结果,并加强对GBS耐药性菌株的监测工作。
Abstract:
Abastract:Objective To investigate the clonization of Streptococcus agalactiae(GBS)in the genitourinary tract of late pregnant women and perform the CAMP negative strains identification and analysis in Guizhouprovince.Methods Collected genital tract secretions fromout-patient women in late pregnancy in the obstetric of Guizhou Provincial People's Hospital during the period from May 2016 to May 2017.Then the collected secretions conducted the bacteria culture and identification.The CAMP-negative GBS strain was furher identified by biochemical test and 16S rDNAsequence analysis,and cfb gene detection was conducted.Results Among the 3 794 samples,118 Streptococcus agalactiaes strains wereisolated,and the positive rate was 3.11%.One of them was beta-hemolytic andthe CFB gene was positive,but the CAMP test was negative,which was confirem tobe Streptococcus agalactiae via 16S rDNA sequence analysis.The results showed that no GBS had been found to be resistant to Penicillin,Amoxicillin,Liniazolamine,Vancomycin,Cefotaxime,Cefepime and the strains with high drug resistencerate were followed by Erythromycin(81.3%),Clindamycin(62.7%),Levofloxacin(72.9%).Conclusion CAMP-negative was extremly rare in Streptococcus agalactiae,and only one strains of the 3 794 samples was isolated.Therefore,the clinical laboratory should be alert to CAMP negatie Streptococcus agalactiae for avoiding omission and providing more accurate test results for clinic.Also,the monitoring of drug-resistant strains of GBS should be strengthen.

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备注/Memo

备注/Memo:
基金项目:黔科合外G字[2014]7017号项目。 作者简介:刘家玲(1988-),女,研究生,主要从事临床微生物检验研究。 通讯作者:袁 军,博士,主任技师,E-mail:1760075125@qq.com。
更新日期/Last Update: 2018-02-07