[1]邓世群,赵世元,张帮献.黏附分子CD44和ICAM-1在肺炎支原体感染BALB/c小鼠体内表达及相关机制[J].现代检验医学杂志,2018,33(01):91-94.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　DENG Shi-qun,ZHAO Shi-yuan,ZHANG Bang-xian.Expression of Adhesion Molecules CD44 and ICAM-1 in Mycoplasma Pneumoniae Infected BALB/c Mice and the Related Mechanisms[J].Journal of Modern Laboratory Medicine,2018,33(01):91-94.[doi:10.3969/j.issn.1671-7414.2018.01.001]
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黏附分子CD44和ICAM-1在肺炎支原体感染BALB/c小鼠体内表达及相关机制()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
91-94
栏目:
论著
出版日期:
2018-02-05

文章信息/Info

Title:
Expression of Adhesion Molecules CD44 and ICAM-1 in Mycoplasma Pneumoniae Infected BALB/c Mice and the Related Mechanisms
文章编号:
1671-7414(2018)01-091-04
作者:
邓世群1; 赵世元2; 张帮献2
1.广西南宁市西乡塘区石埠卫生院检验科,南宁 530008; 2.广西医科大学附属民族医院检验科,南宁 530001
Author(s):
DENG Shi-qun1; ZHAO Shi-yuan2; ZHANG Bang-xian2
1.Department of Clinical Laboratory,Guangxi Nanning Xixiangtang District Shibu Hospital, Nanning 530008,China; 2.Department of Clinical Laboratory,the Affiliated National Hospital of Guangxi Medical University National Hospital,Nanning 530001,China
关键词:
肺炎支原体;  黏附分子;  CD44;  细胞间黏附分子-1
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 研究黏附分子CD44和细胞间黏附分子1(ICAM-1)在肺炎支原体(MP)感染的BALB/c小鼠模型的表达,阐明其与MP感染的关系。方法 采用MP鼻腔滴入法建立BALB/c小鼠MP模型,观察MP小鼠肺部的病理变化。采用ELISA法测定5,8,15,20和30天MP感染小鼠血清和肺泡灌洗液中黏附分子CD44和ICAM-1的表达水平。结果 与模型组比较,MP感染BALB/c小鼠后肺部炎症明显,MP感染BALB/c小鼠后其血清和肺泡灌洗液中黏附分子CD44和ICAM-1的表达明显上调(t5天 血清CD44=53.64 ng/L,t5天 BALF CD44=144.3 ng/L; t5天 血清ICAM-1=73.72 ng/L,t5天 BALF ICAM-1=165.06 ng/L,均P值<0.000; t8天 血清CD44=40.86 ng/L,t8天BALF CD44=21.31 ng/L; t8天血清ICAM-1=30.57 ng/L,t8天BALF ICAM-1=19.61 ng/L,均P值<0.000)。结论 MP感染使小鼠黏附分子CD44和ICAM-1的表达水平升高,用替加环素治疗MP小鼠后CD44和ICAM-1表达下调,CD44和ICAM-1表达上调可能与MP肺炎的严重程度有关。
Abstract:
Abstract:Objective To study the expression of adhesion molecules CD44 and ICAM-1 in BALB/c mice infected with Mycoplasma pneumoniae(MP)and elucidate the relationship between MP and infection.Methods BALB/c mouse MP model was established by MP nasal instillation,and the pathological changes of lung in MP mice were observed.The expression levels of adhesion molecules CD44 and ICAM-1 in serum and bronchoalveolar lavage fluid of mice infected with 5,8,15,20 and 30d MP were determined by ELISA.Results Compared with the model group,MP infected BALB/c mice after lung inflammation was significantly up-regulated MP,the expression in BALB/c mice infected with the serum and bronchoalveolar lavage fluid in CD44 and adhesion molecule ICAM-1(t5 d serum CD44=53.64 ng/L,t5 d BALF CD44=144.3 ng/L; t5 d serum ICAM-1=73.72 ng/L,t5 d BALF ICAM-1=165.06 ng/L,all P<0.000; t8 d serum CD44=40.86 ng/L,t8 d BALF CD44=21.31 ng/L; t8 d serum ICAM-1=30.57 ng/L,t8 d BALF ICAM-1=19.61 ng/L,all P<0.000).The Mycoplasma pneumoniae infection increased the expression levels of adhesion molecules CD44 and ICAM-1 in mice.Conclusion After MP mice were cured,the expression of CD44 and ICAM-1 was down regulated,and the up regulation of CD44 and ICAM-1 expression might be related to the severity of MP pneumonia.

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备注/Memo

备注/Memo:
基金项目:广西医疗卫生科研课题(2015066)。 作者简介:邓世群(1964-),男,学士,主管检验师,主要从事医学检验工作及研究,E-mail:652582452@qq.com。 通讯作者:赵世元(1965-),男,博士,主任检验技师,主要从事药物开发研究,E-mail:zhaoshiyuan_1105@163.com。
更新日期/Last Update: 2018-02-07