[1]赵英伦,马 元,莫 森,等.腰椎间盘突出症患者血清中Caspase-3和Caspase-9活性研究[J].现代检验医学杂志,2018,33(02):13-15.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　ZHAO Ying-Lun,MA Yuan,MO Sen,et al.Serum Caspase-3 and Caspase-9 Activity in Patients with Lubar Intervertebral Disc Protrusion[J].Journal of Modern Laboratory Medicine,2018,33(02):13-15.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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腰椎间盘突出症患者血清中Caspase-3和Caspase-9活性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
13-15
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Serum Caspase-3 and Caspase-9 Activity in Patients with Lubar Intervertebral Disc Protrusion
文章编号:
1671-7414(2018)02-013-03
作者:
赵英伦; 马 元; 莫 森; 李东冉; 王 军; 刘 冲; 詹新立
广西医科大学第一附属医院脊柱骨病外科,南宁 530021
Author(s):
ZHAO Ying-Lun; MA Yuan; MO Sen; LI Dong-Ran; WANG Jun; LIU Chong; ZHAN Xin-Li
Department of Spine and Osteopathy Ward, the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China
关键词:
腰椎间盘突出症;  腰椎退行性病变;  细胞凋亡;  caspase-3;  caspase-9
分类号:
R681.53; R392.12
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 探究腰椎间盘突出症与凋亡相关因子caspase-3和caspase-9的关系。方法 随机选择2014年12月～2016年5月在广西医科大学第一附属医院脊柱骨病外科住院手术治疗的腰椎间盘突出症患者99例,其中单个节段突出的患者70例(A组)、多个节段突出(腰椎间盘突出节段数量≥2个)的患者29例(B组),同时随机选取同期健康体检者40例作为对照组。酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测腰椎间盘突出症患者血清中caspase-3和caspase-9浓度。结果 对照组、A组和B组血清中caspase-3浓度分别为11.24±0.41,14.31±0.67和17.43±1.86 pmol/L,各组之间caspase-3浓度差异具有统计学意义(F=8.47,P<0.01)。对照组、A组、B组血清中caspase-9浓度分别为18.54±2.19,30.57±3.63和43.68±5.15 pmol/L,各组之间caspase-9浓度差异具有统计学意义(F=7.85,P=0.001)。A组(q=3.08,3.29,均P<0.05)、B组(q=5.78,4.50,均P<0.05)caspase-3和caspase-9浓度均高于对照组,差异具有统计学意义,且B组高于A组,差异具有统计学意义(q=3.21,3.22,均P<0.05)。结论 腰椎间盘突出症患者血清caspase-3和caspase-9表达上调促进了细胞凋亡过程,参与了腰椎间盘突出症的发生,并且与椎间盘突出节段数量相关,突出节段数量越多,caspase-3和caspase-9表达量越高。
Abstract:
Abstract:Objective To explore the relationship between apoptosis promoting effector molecules caspase-3,caspase-9 and lubar intervertebral disc protrusion.Methods 99 of operation patients with lubar intervertebral disc protrusion in their maternity ward were recruited.Among them,single segment of lubar intervertebral disc protrusion were 70(Group A),more than one segments of lubar intervertebral disc protrusion were 29(Group B).In addition,40 unrelated healthy people from physical examination center were enrolled as controls.Enzyme-linked immunosorbent assay(ELISA)was used to examine serum caspase-3 and caspase-9 levels in lubar intervertebral discprotrusion patients.Results The level of caspase-3 incontrol group,group A and group B,respectively were 11.24±0.41,14.31±0.67 and 17.43±1.86 pmol/L.The caspase-3 activity in each group was statistically significant difference(F=8.47,P<0.01).The level of caspase-9 in control group,group A and group B respectively were 18.54±2.19,30.57±3.63and 43.68±5.15 pmol/L.The caspase-9 activity in each group was statistically significant difference(F=7.85,P=0.001).Compared with control group,the caspase-3 and caspase-9 activity in group A(q=3.08,3.29,all P<0.05),group B(q=5.78,4.50,all P=0.014)was statistically significant difference.The caspase-3 and caspase-9 activity in group A,group B was statistically significant difference(q=3.21,3.22,all P<0.05).Conclusion The augment of caspase-3 and caspase-9 promoted apoptosis of lubar intervertebral disc protrusion.It was connected with quantity of protrusive segments.The more segments of protrusion,the higher caspase-3 and caspase-9 levels of examination would be.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(No.81560359),广西自然科学基金项目(NO.2014GXNSFAA118173)资助。 作者简介:赵英伦(1992-),男,硕士,住院医师,研究方向:骨科,E-mail:834904502@qq.com。 通讯作者:詹新立(1970-),E-mail:3cstar@163.com。
更新日期/Last Update: 2018-04-16