[1]李晓锋,刘 希,张冠军,等.ARMS法检测肺腺癌EGFR基因突变及临床意义[J].现代检验医学杂志,2018,33(02):42-45.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　LI Xiao-feng,LIU XI,ZHANG Guan-jun,et al.Detection of EGFR Gene Mutation in Lung Adenocarcinoma by ARMS and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2018,33(02):42-45.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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ARMS法检测肺腺癌EGFR基因突变及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
42-45
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Detection of EGFR Gene Mutation in Lung Adenocarcinoma by ARMS and Its Clinical Significance
文章编号:
1671-7414(2018)02-042-04
作者:
李晓锋; 刘 希; 张冠军; 汪园园
西安交通大学第一附属医院病理科,西安 710061
Author(s):
LI Xiao-feng; LIU XI; ZHANG Guan-jun; WANG Yuan-yuan
Department of Pathology, the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China
关键词:
扩增阻滞突变系统;  肺腺癌;  表皮生长因子受体;  肺癌原发部位
分类号:
R734.2; R730.43
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 探讨扩增阻滞突变系统(ARMS)法在肺腺癌中表皮生长因子受体(EGFR)基因突变检测的应用及其临床意义。方法 收集2015年1月～2016年8月西安交通大学第一附属医院病理科的肺腺癌标本566例作为研究对象。其中,胸腔积液细胞块标本34例,肺活检标本401例,手术切除标本131例,采用ARMS法进行石蜡标本EGFR基因突变检测,分析EGFR基因突变与肺腺癌患者临床资料的相关性。结果 肺腺癌标本566例中,吸烟腺癌患者239例,非吸烟腺癌患者327例。吸烟患者中,EGFR突变率与患者年龄、性别、手术方式等无明显关联(P>0.05),而与肺癌原发部位关系密切(P<0.05); 非吸烟患者中,EGFR突变率与性别、年龄、标本类型及肺癌原发病灶部位无明显相关性(P>0.05)。结论 ARMS法可有效应用于肺腺癌临床病理石蜡标本中EGFR基因突变检测。吸烟是EGFR突变率的重要影响因子,且对左、右肺均有影响,但是对右肺的影响较大。
Abstract:
Abstract:Objective To investigate the efficacy and clinical significance of amplification refractory mutation system(ARMS)in epidermal growth factor receptor(EGFR)gene mutation in lung adenocarcinoma.Methods Collected 566 specimens of lung adenocarcinomia in pathology from Department of the First Affiliated Hospital of Xi'an Jiaotong University from January 2015 to August 2016.As the research object,which included 34 cases of pleural cell specimens,401 cases of lung biopsy specimens and surgical specimens from 131 patients with ARMS to complete the above specimens EGFR gene mutation detection,analysis of EGFR gene mutations associated with non-small cell lung cancer patients clinical data.Results Among 566cases of lung cancer specimens,the EGFR mutation rate of 239 cases of patientswith smoking had no obvious correlation with age,gender and surgical methods(P>0.05),but primary lung site was closely related(P<0.05),and EGFR mutation rate of 327 cases of patients with non smoking had no obvious correlationwithage,sex,operation mode and primary lung site(P>0.05).Conclusion ARMS is an ideal method for the detection of EGFR gene mutation in non-small cell lung cancer.Smoking is a great influence on EGFR mutation rate in both lung tissue,and for right lung tissue is more dramatic.

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备注/Memo

备注/Memo:
基金项目:陕西省国际科技合作与交流计划(项目编号:2016KW-009)。 作者简介:李晓锋(1978-),男,本科,主管技师,主要从事工作及研究方向:肿瘤分子病理,E-mail:15291816606@163.com。 通讯作者:汪园园(1988-),女,硕士,实习研究员,主要从事工作及研究方向:肿瘤分子病理,E-mail:1051815925@qq.com。
更新日期/Last Update: 2018-04-16