[1]江 凡,王飞玲,路瑞静,等.深圳地区人群B群链球菌血清学分布和毒力基因及耐药性分析[J].现代检验医学杂志,2018,33(02):51-54.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　JIANG Fan,WANG Fei-ling,LU Rui-jing,et al.Analysis of Serological Distribution,Virulence Gene and Drug Resistance among Group B Streptococcus from Shenzhen Patients[J].Journal of Modern Laboratory Medicine,2018,33(02):51-54.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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深圳地区人群B群链球菌血清学分布和毒力基因及耐药性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
51-54
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Analysis of Serological Distribution,Virulence Gene and Drug Resistance among Group B Streptococcus from Shenzhen Patients
文章编号:
1671-7414(2018)02-051-04
作者:
江 凡; 王飞玲; 路瑞静; 周喜友; 邹建话
深圳市宝安区妇幼保健院检验科,广东深圳 518101
Author(s):
JIANG Fan; WANG Fei-ling; LU Rui-jing; ZHOU Xi-you; ZOU Jian-hua
Department of Laboratory Medicine, Maternity and Child Health Care Institute of Baoan District,Guangdong Shenzhen 518101,China
关键词:
B群链球菌;  分子分型;  毒力基因;  耐药性
分类号:
R378.12; R446.5
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 了解深圳地区B群链球菌(GBS)血清型分布、毒力基因和耐药性。方法 来自深圳4家医院515例B群链球菌(Group B Streptococcus,GBS)分离株(其中108例侵袭性,112例非侵袭性和295例定植株)。所有GBS分离株采用常规血清方法和分子方法进行血清型分型; PCR方法检测GBS分离株的scpB,lmb,hylB,cylE,bac,bca和rib毒力基因; 纸片扩散法检测GBS对青霉素、红霉素和四环素的敏感度; 青霉素的最低抑菌浓度(MICs)用E实验检测。结果 分子分型(8个血清型Ⅰa,Ⅰb,Ⅱ～Ⅵ,Ⅸ)与血清分型相符。结合分子分型与血清学分型,血清型Ⅲ是最常见的荚膜型(56.5%),然后依次为Ⅰb(17.5%),Ⅰa(12.6%),V(7.4%),Ⅱ(2.7%),Ⅵ(1.4%),Ⅳ(1.0%)和Ⅸ(1.0%)。在不同的人群中Ⅲ型为主要血清型。除Ⅲ型外,在侵袭性和非侵袭性感染人群中,血清型Ⅰa也较为常见(11.1%和29.5%),Ⅰb在定植人群中较为常见(19.3%)。PCR检测毒力基因表明在几乎所有分离株中含cylE,lmb,scpB和hylB基因,在515例分离株中发现rib,bca和bac基因分别为29.1%,14.6%和9.7%。此外,一些毒力基因显著与特定的血清型相关,如rib与血清型Ⅲ,Ⅰa和Ⅰb主要相关; bca和bac与血清型Ⅲ和Ⅰb主要相关。药敏结果显示,GBS对β-内酰胺酶100%敏感,对红霉素、克林霉素和四环素的耐药性分别为67.0%,61.9%和86.0%。结论 GBS血清型分布、毒力基因和耐药性的相关性为临床治疗、流行病学研究和疫苗设计方面提供重要依据。
Abstract:
Abstract:Objective To understand the antimicrobial susceptibility profiles,serotype distribution and virulence genes.Methods A total of 515 group B Streptococcus(GBS)including 108 virulence,112 non-virulence,and 295 colonizing isolates were collected in four Shenzhen hospitals.Isolates were characterized by conventional and molecular serotyping.The virulence genes of scpB,lmb,hylB,cylE,bac,bca and rib of GBS isolates were detected by PCR.Antimicrobial susceptibility to penicillins,macrolides,lincosamides,quinolones and tetracyclines was tested using disk diffusionand the MICs for penicillin were determined by E-test.Results Molecular serotyping for all eight serotypes(Ⅰa,Ⅰb,Ⅱ～Ⅵ,Ⅸ)was in full accordance with conventional serotyping.Taking MS and CS together,serotype Ⅲ was the most common capsular type(56.5%),followed by Ⅰb(17.5%),Ⅰa(12.6%),V(7.4%),Ⅱ(2.7%),Ⅵ(1.4%),Ⅳ(1.0%)and Ⅸ(1.0%).Serotype Ⅲ was the main serotype in different groups,serotype Ⅰa was significantlymore common among patients with invasive infections(11.1%)and no-invasive infections(29.5%),serotype Ⅰb isolates were significantly more common among clone(19.3%).Virulence gene screening using PCR method showed the presence ofcylE,lmb,scpB and hylB in almost all the isolates,while rib,bca and bac genes were found in 29.1%,14.6% and 9.7% of the isolates.Certain genes were significantly associated with specific serotypes,for example,rib with serotypesⅢ,Ⅰa andⅠb,bca and bac with serotypes Ⅲ and Ⅰb.Drug susceptibility results showed that GBS susceptibility to β-lactam antimicrobials was prevalent(100%).Resistance rates for erythromycin,clindamycin and tetracycline were 67.0%,61.9% and 86.0%,respectively.Conclusion Serotype distribution,virulence genes and antimicrobial susceptibility profiles of GBS contributes to the clinical therapy,epidemiological studies and design of Vaccines.

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备注/Memo

备注/Memo:
基金项目:深圳市知识创新计划项目(JCYJ20150403105513694)。 作者简介:江 凡(1969-),男,本科,副主任技师,研究方向:检验方法学,E-mail:jiangfan1224@163.com。 通讯作者:邹建话(1963-),本科,主任技师,研究方向:医学检验,E-mail:BAFYJY@163.com。
更新日期/Last Update: 2018-04-16