[1]吴 意,金 娴,樊春卉,等.儿童呼吸道合胞病毒感染血清特异性抗体IgM,IgG和IgA表达的相关性研究[J].现代检验医学杂志,2018,33(02):82-85.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　WU Yi,JIN Xian,FAN Chun-hui,et al.Correlation Research of Serum Specific Antibody Expression of IgM,IgG and IgA in Children with Respiratory Syncytial Virus Infection[J].Journal of Modern Laboratory Medicine,2018,33(02):82-85.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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儿童呼吸道合胞病毒感染血清特异性抗体IgM,IgG和IgA表达的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
82-85
栏目:
论著
出版日期:
2018-04-05

文章信息/Info

Title:
Correlation Research of Serum Specific Antibody Expression of IgM,IgG and IgA in Children with Respiratory Syncytial Virus Infection
文章编号:
1671-7414(2018)02-082-04
作者:
吴 意; 金 娴; 樊春卉; 侯家兴; 赵 毅
深圳市第七人民医院检验科,广东深圳 518081
Author(s):
WU Yi; JIN Xian; FAN Chun-hui; HOU Jia-xing; ZHAO Yi
Department of Clinical Laboratory, Shenzhen Seventh People's Hospital,Guangdong Shenzhen 518081,China
关键词:
呼吸道合胞病毒;  抗体;  表达;  辅助诊断
分类号:
R373.14; R392.11
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 探讨住院儿童呼吸道合胞病毒(RSV)感染血清特异性抗体IgM,IgG和IgA表达的相关性,筛选具有早期辅助诊断意义的抗体指标。方法 运用荧光定量聚合酶链反应技术(FQ-PCR)筛选出2015～2017年50例咽拭子RSV阳性的住院患儿; 采用酶联免疫吸附试验(ELISA)检测患儿血清中的特异性抗体IgM,IgG和IgA; 同时以95例无呼吸道感染症状的儿童血清标本作为对照组; 采用卡方检验对结果进行统计学分析。结果 50例咽拭子RSV阳性患儿血清中IgM,IgG,IgA及三者同时出现阳性率分别为24.00%,60.00%,22.00%%和16.00%,差异有统计学意义(χ2=28.19,P<0.01); 同一性别患儿的IgM,IgG和IgA阳性率在实验组和对照组中差异有统计学意义(χ2=9.16,P<0.01),不同性别在同一实验组或对照组中各抗体阳性率差异无统计学意义(χ2=0.10,P>0.05); 急性喉气管支气管炎中未检出IgM,IgG和IgA,仅在急性上呼吸道感染中检测到1例IgG,支气管肺炎及急性支气管炎中以IgG的检出率41.38%和23.53%最高,且均未单独检测到IgA; <6个月年龄组患儿在7天和21天内均未检出IgM和IgA,1～5岁年龄组患儿在7天内产生IgM阳性率为50.00%最高,且在21天内均能检出IgM,IgG和IgA,5～10岁年龄组患儿在7天内产生IgG和IgA的阳性率为100%和66.67%。结论 RSV特异性抗体IgM,IgG和IgA不能单独作为早期感染RSV的诊断指标,特异性抗体产生不受性别因素影响,上呼吸道感染RSV较难产生IgM,IgG和IgA,患儿年龄越小产生IgM和IgA的速度越慢,同时IgG存在母婴垂直传播且对机体无保护作用,RSV感染人体引起临床呼吸道症状可能与自身免疫力有关; IgA产生最早且不单独出现。
Abstract:
Abstract:Objective To explore the correlation between the expression of serum specific antibody IgM,IgG and IgA in the respiratory syncytial virus(RSV)in hospitalized children,and the antibody indexes with earlyauxiliary diagnostic significance were screened.Methods Using fluorescent quantitative polymerase chain reaction(FQ-PCR)to screen 50cases of throat swabs which RSV-RNA were positive in hospitalized children from2015 to 2017 and using enzyme-linked immunosorbent assay(ELISA)detected specific antibodies IgM,IgG and IgA in the children's serum.Meanwhile 95 cases ofchildren's serum specimens without respiratory symptoms were taken as the control group.The results were analyzed by chi-square test.Results In the 50 cases of serum from children who's RSV-RNA were positive from throat swabs,the positive rates of IgM,IgG,IgA and the three forms comingtogether were 24.00%,60.00%,22.00% and 16.00% respectively.And the difference was statistically significant(χ2=28.19,P<0.01).About the serumswhich were the same gender child patient in the experimental group and the control group,the positive rates of IgM,IgG and IgA had significant differences(χ2=9.16,P<0.01).There was no significant difference in the positive rate of each antibody in the same experimental group or control group or controlgroup(χ2=0.10,P>0.05).IgM,IgG and IgA were not detected in acute laryngotracheal bronchitis.Only 1 case with IgG was detected in the acute upper respiratory infection.It was the highest that the detection rates of IgG in bronchial pneumonia and acute bronchitis were 41.38% and 23.53%,and IgA was not been detected alone.In the group of <6 months,IgM and IgA were not detected within 7 days and 21 days.In the group of 1～5 years old,the positive rate of IgMwithin 7 days was 50%,which was the highest in all groups.And IgM,IgG and IgA could be detected in 21 days also.The positive rates of IgG and IgA in the age group of 5～10 years old were 100% and 66.67% respectively.Conclusion Specific antibodies of RSV-IgM,IgG and IgA cannot be used asa diadynamic criteria alone in early RSV infection.The specific antibody was not affected by gender.Upper respiratory tract infection RSV was difficult to produce IgM,IgG and IgA.The younger the child,was the slower the IgM and IgA were produced.At the same time,IgG was able to vertical transmission and it had no protective effect on body.That RSV could infect the body and cause clinical respiratory symptoms would be related with immunity.Finally,IgA was the earliest specific antibody and could not be alone.

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备注/Memo

备注/Memo:
基金项目:深圳市盐田区科技局科技计划资助项目(20160102)。 作者简介:吴 意(1982-),男,本科学历,主管检验师,主要从事病原微生物分子诊断学研究,E-mail:845007571@qq.com。
更新日期/Last Update: 2018-04-16