[1]浩日瓦,娜日罕,周 红,等.血清microRNA126和microRNA1检测对心衰诊断价值的研究[J].现代检验医学杂志,2018,33(03):18-20/23.[doi:10.3969/j.issn.1671-7414.2018.03.006]
　HAO Ri-wa,NA Ri-han,ZHOU Hong,et al.Stydy on the Diagnostic Value of Detecting Serum MicroRNA126 and MicroRNA1 in Heart Failure[J].Journal of Modern Laboratory Medicine,2018,33(03):18-20/23.[doi:10.3969/j.issn.1671-7414.2018.03.006]
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血清microRNA126和microRNA1检测对心衰诊断价值的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年03期
页码:
18-20/23
栏目:
论著
出版日期:
2018-07-17

文章信息/Info

Title:
Stydy on the Diagnostic Value of Detecting Serum MicroRNA126 and MicroRNA1 in Heart Failure
文章编号:
1671-7414(2018)03-018-04
作者:
浩日瓦; 娜日罕; 周 红; 董 莉
内蒙古医科大学附属医院检验科,呼和浩特 010050
Author(s):
HAO Ri-wa; NA Ri-han; ZHOU Hong; DONG Li
Department of Clinical Laboratory, the Affiliated Hospital of Inner Mongolia Medical University,Hohehot 010050,China
关键词:
microRNA126; microRNA1;  心肌梗死;  扩心病;  心衰
分类号:
R541.6; R392.11
DOI:
10.3969/j.issn.1671-7414.2018.03.006
文献标志码:
A
摘要:
目的 探讨血液中microRNA-126(miR-126)和microRNA-1(mir-1)在心衰患者中的诊断及预后评估价值。方法 选择2015年9月～2017年1月心内科住院病人心梗后心衰患者(AMI组)45例,扩心病心衰患者(DCM组)30例,健康体检者(健康对照组)20例,采用定量反转录聚合酶链反应(qRT-PCR)检测其血清中miR-126和miR-1的相对含量,进行统计学分析。结果 ①患者组与对照组的miR-126和miR-1分别为0.76±0.012 vs 1.01±0.145和0.54±0.09 vs1.27±0.649,与对照组比较,患者组显著低于对照组,差异具有统计学意义(t=72.14,38.05,均P<0.001); AMI组与DCM组相比较,差异无统计学意义(均P>0.005)。②患者组中miR-126,miR-1与NTproBNP,cTnT,心胸比均呈负相关,与LVEF呈正相关,相关系数分别为:-0.427,-0.578; -0.716,-0.534; -0.311,-0.631; 0.557和0.435,均P<0.005; ③miR-126和miR-1诊断心衰的ROC曲线下面积为0.52和0.51,最佳cutoff值分别为0.549和0.53,灵敏度为55%和65.3%,特异度为85%和78.2%,准确度为87.1%和90.2%,联合检测NTproBNP和cTnT诊断心衰的灵敏度、特异度分别为69.3%和92.6%。④血清miR-1,miR-126水平与患者1年主要不良心脏事件(MACE)及死亡率无关,相对危险度为(2.35,0.53,P>0.005)。结论 用ROC曲线分析的结果表明,miR-126和miR-1对于心衰具有诊断价值,联合NTproBNP和cTnT对心衰诊断和治疗更有意义,但不能用于心衰病因的区分,且对患者1年的预后及不良事件预测无意义。
Abstract:
Abstract:Objective To evaluate the values of plasma microRNA-126,microRNA-1 on diagnosis and prognosis for heart failure after myocardial infarction and dilated cardiomyopathy patients.Methods Chose 45 cases of patients with heart failure after myocardial intarction(AMI)and 30 cases of enlarged heart disease in patients with heart failure(DCM)from hospitalized patients in department of cardiology from September 2015 to January 2017 as observation group,and 20 health check-ups as the control group.Used the quantitative reverse transcription polymerase chain reaction(qRT-PCR)to detect the relative content of serum miR-126 and miRi-1,doing statisticalanalysis.Results ①miR-126 and miR-1 of patients group and control group were 0.76±0.012 vs 1.01±0.145 and 0.54±0.09 vs 1.27±0.649,respectively,compared with control group,the patient group was significantly lower than the control group(t=72.14,38.05,P<0.001),havingsignificantly statistical difference,and compared with the DCM group,the AMIgroup had no statistical significance(P>0.005).②In the patients group,mir-126,mir-1 and NTproBNP,cTnT,and cardiothoracic ratio were negatively correlated,positively correlated with LVEF,and the correlation coefficients were-0.427 and 0.578,-0.716 and 0.534,-0.311 and 0.631,0.557 and 0.435(all P<0.005).③The area under the ROC curve of miR-126 and miR-1 was 0.52 and 0.51.The optimal cutoff values were 0.549 and 0.53 respectively.Sensitivity was 55% and 65.3%,specificity was 85% and 78.2%,accuracy was 87.1%and 90.2%,and the sensitivity and specificity of NTproBNP and cTnT diagnosisheart failure were 69.3% and 92.6% respectively.④Serum miR-1 and miR-126 levels were not related to the patients' major adverse cardiac events(MACE)andmortality in 1 year.The relative risk was 2.35 and 0.53(P>0.005).Conclusion Circulating miR-126 and miR-1 can be used as a supplement biomarker for HF diagnosis,and cannot be as a biomarker of antidiastole.

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备注/Memo

备注/Memo:
基金项目:内蒙古自治区自然基金(2017MS0826),内蒙古自治区科技厅科技计划基金(2016)。 作者简介:浩日瓦(1992-),男,在读硕士研究生,研究方向:心血管疾病检验诊断学。 通讯作者:董 莉(1964-),女,主任检验师,硕士研究生导师。
更新日期/Last Update: 2018-04-16