[1]李腾达,刘 鹏,龙曙萍,等.表面增强拉曼光谱技术检测隐球菌脑膜炎患者血清中CD93+外泌体及其意义[J].现代检验医学杂志,2018,33(04):4-7.[doi:10.3969/j.issn.1671-7414.2018.04.002]
　LI Teng-da,LIU Peng,LONG Shu-ping,et al.Detection of Serum CD93+Exosomes in Patients with Cryptococcal Meningitis by Surface-Enhanced Raman Scattering Technology and Its Significance[J].Journal of Modern Laboratory Medicine,2018,33(04):4-7.[doi:10.3969/j.issn.1671-7414.2018.04.002]
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表面增强拉曼光谱技术检测隐球菌脑膜炎患者血清中CD93+外泌体及其意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
4-7
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Detection of Serum CD93+Exosomes in Patients with Cryptococcal Meningitis by Surface-Enhanced Raman Scattering Technology and Its Significance
文章编号:
1671-7414(2018)04-004-04
作者:
李腾达1; 刘 鹏1; 龙曙萍1; 刘 云1; 黄元兰2; 张薇薇1; 郭 杰1; 谷明莉1; 邓安梅1
1.第二军医大学长海医院,上海 200433; 2.解放军455医院,上海 200052
Author(s):
LI Teng-da1; LIU Peng1; LONG Shu-ping1; LIU Yun1; HUANG Yuan-lan2; ZHANG Wei-wei1;  GUO Jie1; GU Ming-li1; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China; 2.No.455 Hospital of PLA,Shanghai 200052,China
关键词:
表面增强拉曼光谱技术;  隐球菌脑膜炎;  外泌体;  CD93
分类号:
Q503; R519.4
DOI:
10.3969/j.issn.1671-7414.2018.04.002
文献标志码:
A
摘要:
目的 利用表面增强拉曼光谱技术(SERS)检测隐球菌脑膜炎患者血清中CD93+外泌体,以优化该病的监测手段。方法 收集2013年12月～2017年3月在上海长海医院和长征医院确诊的38例隐球菌脑膜炎患者的血清作为实验组,同期体检的38例健康者血清作为对照组。用ELISA法检测血清中的IFN-γ,TNF-α的蛋白表达水平; 用超高速离心法分离血清外泌体; 用磁珠连接抗体法捕获CD93+外泌体并进行标记,于拉曼光谱仪下检测样本的SERS信号。实验组与对照组计量资料间的比较采用两独立样本的t检验,用Origin75软件对两连续变量进行线性拟合,两变量之间的相关性以Pearson系数表示。结果 SERS检测隐球菌脑膜炎患者血清中外泌体最低浓度为5 500个/ml,最低量为110个/ml,log(SERS信号)与log(外泌体浓度)进行线性拟合的R值为0.995 96,P<0.0001; SERS检测实验组与对照组血清外泌体CD93的信号值取对数分别为2.75±0.59 vs 2.47±0.15,差异具有统计学意义(t=2.835,P=0.005 9); SERS检测实验组血清CD93+外泌体得到的数据与ELISA法检测实验组血清中IFN-γ,TNF-α的结果呈正相关,差异有统计学意义(r=0.389,0.478,P<0.05)。结论 该方法可初步提高隐球菌脑膜炎患者血清标本检测的灵敏度,为该病监测提供新的技术思路。
Abstract:
Abstract:Objective To detect the serum CD93+exosomes of patients with cryptococcal meningitis by Surface-enhanced Raman Scattering technology(SERS)in order to optimize the measures to monitor this disease.Methods The serum samples of 38 patients with cryptococcal meningitis diagnosed in Changhai Hospital and Changzheng Hospital were collected from December 2013 to March 2017.In the same period,the serum samples of 38 health subjects were collected as control group.The protein expression level of IFN-γ,TNF-α in serums were examined by ELISA method.The exosomes in serum were separated by the ultracentrifuge method.The CD93+exosomes were captured bymagnetic bead-connected antibodies.Under the Raman spectroscopy,the SERS signals of samples was acquired.The comparison of measure data between experimentaland controlled groups was measured by two independent samples' t test,Origin 75 software was used to do the linear fitting for two continuous variables,and the relationship between the two variables was measured by Pearson coefficient.Results The lowest concentration of serous exosomes in patients with cryptococcal meningitis that could be tested by SERS was 5 500 particles/ml,the lowest counts were 110 particles/ml.The R value for linearfitting of log(SERS signal)and log(exosomes' concentration)was 0.995 96(P<0.000 1).The log values of SERS signals tested for CD93 on serous exosomes in experimental and controlled groups were 2.75±0.59 vs 2.47±0.15(t=2.835,P=0.005 9),with statistical difference.The results from the CD93+exosomes in serum samples of experiment group were positively related withthe ELISA results of IFN-γ and TNF-α in serum samples of experiment group and there was statistical difference(r=0.389,0.478,P<0.05).Conclusion This method can primarily improve the sensitivity of the detection of serum samples in patients with cryptococcal meningitis and may provide a new technological way to detect this disease.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81671556,81601406,81471605,81501397,31500721,81501398,81401358,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:李腾达(1990-),女,在读硕士研究生,主要从事自身免疫研究,E-mail:tengdali@smmu.edu.cn。 刘 鹏(1982-),女,在读博士研究生,主要从事自身免疫研究,E-mail:ppliu23@163.com,共同第一作者。 通讯作者:邓安梅,女,教授,E-mail:amdeng70@163.com。 谷明莉,女,检验技师,E-mail:mingligu@126.com,共同通讯作者。
更新日期/Last Update: 2018-08-16