[1]王 倩,劳淑贞,朴金松,等.乳腺良恶性肿瘤组织中CD44的表达及其与淋巴管生成的关系[J].现代检验医学杂志,2018,33(04):16-1.[doi:10.3969/j.issn.1671-7414.2018.04.005]
　WANG Qian,LAO Shu-zhen,PIAO Jin-song,et al.Expression of CD44 in Benign and Malignant Breast Tumors and Its Relationship with Lymphangiogenesis[J].Journal of Modern Laboratory Medicine,2018,33(04):16-1.[doi:10.3969/j.issn.1671-7414.2018.04.005]
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乳腺良恶性肿瘤组织中CD44的表达及其与淋巴管生成的关系()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
16-1
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Expression of CD44 in Benign and Malignant Breast Tumors and Its Relationship with Lymphangiogenesis
文章编号:
1671-7414(2018)04-016-04
作者:
王 倩; 劳淑贞; 朴金松; 高蔚樱; 赵 凯; 张东辉; 谭小军; 罗 凯
广州医科大学附属肿瘤医院/广州医科大学肿瘤研究所,广州 510095
Author(s):
WANG Qian; LAO Shu-zhen; PIAO Jin-song;  GAO Wei-ying; ZHAO Kai; ZHANG Dong-hui; TAN Xiao-jun; LUO Kai
Affiliated Cancer Hospital of Guangzhou Medical University/Institute of Oncology of Guangzhcu Medical University,Guangzhou 510095,China
关键词:
乳腺肿瘤;  CD44;  淋巴管生成;  免疫组织化学
分类号:
R737.9; R730.43
DOI:
10.3969/j.issn.1671-7414.2018.04.005
文献标志码:
A
摘要:
目的 研究细胞黏附分子44(CD44)在乳腺良恶性肿瘤组织中的表达及其与淋巴管生成的关系,探讨其在鉴别诊断中的应用价值。方法 收集2005～2015年广州医科大学附属肿瘤医院乳腺肿瘤患者组织样品75例(均为女性)并分为良性肿瘤组、乳腺浸润性导管癌淋巴结转移组和未转移组,每组各25例。免疫组织化学法(IHC)检测CD44在各组患者肿瘤组织样品中的表达并以CD44作为淋巴管内皮细胞标记物比较各组样品中的淋巴管生成情况。生物信息分析验证研究结果。结果 在良性肿瘤组、乳腺浸润性导管癌淋巴结转移组和未转移组中,CD44的IHC评分结果分别为0.60±0.82,3.16±2.70和3.52±2.47,乳腺浸润性导管癌淋巴结转移组和未转移组的CD44表达水平均高于乳腺良性肿瘤组,差异具有统计学意义(F=13.52,P<0.000 1)。以CD44作为淋巴管内皮细胞特异性标记物可有效辅助三组样品的淋巴管生成计数。在乳腺良性肿瘤组、乳腺浸润性导管癌伴淋巴结转移组和未伴淋巴结转移组中,淋巴管生成数分别为4.08±2.43,13.80±13.54和12.72±13.69,乳腺浸润性导管癌淋巴结转移组和未转移组的淋巴管生成数均高于乳腺良性肿瘤组,差异具有统计学意义(F=4.94,P=0.009 7)。生物信息分析结果也显示CD44在乳腺癌中的表达较正常乳腺增加。结论 乳腺癌的发生与CD44表达以及淋巴管的生成有关。CD44有望成为乳腺良性肿瘤与乳腺癌的辅助鉴别诊断指标,同时其也可作为乳腺淋巴管内皮细胞的特异性标记物。
Abstract:
Abstract:Objective To study the expression of CD44 in benign and malignant breast tumors and its relationship with lymphangiogenesis,and explore its application value in differential diagnosis.Methods 75 cases(all females)of breast tumor patients from Affiliated Cancer Hospital of Guangzhou Medical University were collected from 2005 to 2015.All patients were divided into benign tumor group,breast invasive ductal carcinoma with lymph node metastasis group and non-metastasis group,25 cases in each group.Immunohistochemistry(IHC)was used to detect the expression of CD44 in tumor tissue samples of each group and the sample lymphangiogenesis in each group was compared used CD44 as a marker of lymphatic endothelial cells.Bioinformatics analysis was used to verify the study results.Results In the benign tumor group,breast invasive ductal carcinoma lymph node metastasis group and non-metastasis group,the IHC scores of CD44 were 0.60±0.82,3.16±2.70 and 3.52±2.47,respectively.The expression of CD44 in the lymph node metastasis group and the non-metastasis group of breast invasive ductalcarcinoma was higher than that in the benign breast tumor group(F=13.52,P<0.000 1).It could effectively assist lymphangiogenesis in all samples of three groups that used CD44 as a specific marker for lymphatic endothelial cells.In the benign breast tumor group,breast invasive ductal carcinoma with lymph node metastasis group and lymph node metastasis group,the number of lymphangiogenesis was 4.08±2.43,13.80±13.54 and 12.72±13.69,respectively.The number of lymphangiogenesis in the lymph node metastasis group and the non-metastasis group of the invasive ductal carcinoma of the breast were higher than those in the benign breast tumor group(F=4.94,P=0.009 7).Bioinformatics analysis also showed that the expression of CD44 in breast cancer was higher than thatin normal breast.Conclusion The occurrence of breast cancer was associated with CD44 expression and lymphangiogenesis.CD44 is expectedto be a differential diagnosis marker of benign breast tumors and breast cancer,and it can also be used as a specific marker for breast lymphatic endothelialcells.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(No.81772825),广州市卫计委医药卫生科技项目(No.20171A011320)。 作者简介:王 倩(1980-),女,硕士,主治医师,乳腺肿瘤病理,E-mail:13798040957@163.com。 通讯作者:罗 凯(1977-),男,博士,副主任技师,肿瘤靶向治疗耐药与分子靶向检测,E-mail:luokainan@126.com。
更新日期/Last Update: 2018-08-16