[1]张 瑞,秦永亮,李 姣,等.白藜芦醇对须癣毛癣菌体外抑制作用和超微结构初步研究[J].现代检验医学杂志,2018,33(05):53-55+60.[doi:10.3969/j.issn.1671-7414.2018.05.015]
　ZHANG Rui,QIN Yong-liang,LI Jiao,et al.Susceptibility Testing and Mechanism Research of Resveratrol against Trichophyton Mentagrophytes[J].Journal of Modern Laboratory Medicine,2018,33(05):53-55+60.[doi:10.3969/j.issn.1671-7414.2018.05.015]
点击复制

白藜芦醇对须癣毛癣菌体外抑制作用和超微结构初步研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年05期
页码:
53-55+60
栏目:
论著
出版日期:
2018-11-18

文章信息/Info

Title:
Susceptibility Testing and Mechanism Research of Resveratrol against Trichophyton Mentagrophytes
文章编号:
1671-7414(2018)05-053-04
作者:
张 瑞; 秦永亮; 李 姣; 郭玉楷; 张曼娜; 李永军
河北医科大学第二医院,石家庄 050000
Author(s):
ZHANG Rui; QIN Yong-liang; LI Jiao; GUO Yu-kai; ZHANGMan-na; LI Yong-jun
the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China
关键词:
白藜芦醇;  须癣毛癣菌;  抑制作用;  超微结构
分类号:
R969; R379.2
DOI:
10.3969/j.issn.1671-7414.2018.05.015
文献标志码:
A
摘要:
目的 体外研究2017年9月～2018年3月收集的15株须癣毛癣菌(trichophyton mentagrophytes,T.mentagrophytes)对白藜芦醇(resveratrol,Res)的药物敏感性及超微结构的影响,推测白藜芦醇可能的抑菌机理。方法 参考美国临床和实验室标准化研究所(clinical and laboratory standards institute,CLSI)M38-A2方案,采用微量液基稀释法测定对照组及实验组不同浓度白藜芦醇体外抗须癣毛癣菌的最低抑菌浓度(minimum inhibitory concentration,MIC),及透射电镜下菌体超微结构的变化。结果 白藜芦醇对须癣毛癣菌的MIC50和MIC90分别为0.064 mg/ml,0.128 mg/ml; 实验组的菌体结构在透射电镜下较正常对照组变化明显:细胞壁剥离、缺如,质壁分离现象严重,且明显肿胀、变形,细胞膜严重皱缩,核膜暴露并膨大,断裂、溶解消失,细胞器完全消失,呈空泡化改变。结论白藜芦醇对须癣毛癣菌有明显的抑制作用,有治疗皮肤癣菌病的应用潜力。
Abstract:
Objective To evaluate the susceptibility of Resveratrol(Res)on Trichophyton mentagrophytes and influence on the ultrastructure,then speculate the antifungal mechanism.Methods Minimum inhibitory concentration(MIC)were determined by Clinical and Laboratory Standards Institute(CLSI)M38-A2 scheme.The ultrastructure was studied by transmission electron microscope(TEM).Results The MIC50 and MIC90 were 0.064 and 0.128 mg/ml respectively.Observation underTEM showed obvious morphological changes:there were a few showing gathering state and membranes because of Res on photogragh of experimental group and clearlybe seen that the fungus had sinking or scarred or distorted body.Conclusion Resveratrol was found to possess intense activity on Trichophyton mentagrophytes,which makes Res having a potent of substituting antidermatophytic drugs.

参考文献/References:

[1] 李 颖,向 瑶,邓惠萍,等.白藜芦醇对花萼海绵诱癌素所致小鼠空间记忆障碍的预防作用[J].中国病理生理杂志,2017,33(5):951-955.
Li Y,Xiang Y,Deng HP,et al.Prevention of resveratrol on spatialmemory loss of mice induced by calyculin A[J].Chinese Journal of Pathophysiology,2017,33(5):951-955.
[2] 王 鑫,张 瑞,李雅静,等.白藜芦醇对金黄色葡萄球菌标准株抑菌作用及超微结构的影响[J].现代检验医学杂志,2016,31(3):55-58.
Wang X,Zhang R,Li YJ,et al.Antibacterial and ultrastructure effect of RES on Staphylococcus aureus standard strains ATCC 25823[J].J Mod Lab Med,2016,31(3):55-58.
[3] Kolouchová L,Matátková O,Paldrychová M,et al.Resveratrol,pterostilbene and baicalein:plant-derived anti-biofilm agents[J].Folia Microbiologica,2018,63(3):261-272.
[4] Lee J,Lee DG.Novel antifungal mechanism of resveratrol:apoptosis inducer in candida albicans[J].Curr Microbiol,2015,70(3):383-389.
[5] Docherty JJ,Fu MM,Tsai M.Resveratrol selectively inhibits Neisseria gonorrhoeae and Neisseria mening-itidis[J].Journal of Antimicrobial Chemotherapy,2001,47(2):243-244.
[6] 王敬茶,魏晓丽,吕新翔,等.石膏样毛癣菌致皮肤癣菌肉芽肿一例[J].中国麻风皮肤病杂志,2016,32(7):423-424.
Wang JC,Wei XL,L(¨overu)XX,et al.Trichophytic granuloma caused by trichophyton mentagrophytes:a case report[J].Chin J Lepr Skin Dis,2016,32(7):423-424.
[7] 陈柏叡,孙 毅,胡小平,等.须癣毛癣菌的形态学及分子生物学鉴定[J].中国真菌学杂志,2010,5(6):321-326.
Chen BR,Sun Y,Hu XP,et al.Morphology and molecular biological identification of Trichophyton mentagrophytes[J].Chin J Mycol,2010,5(6):321-326.
[8] Clinical and Laboratory Standards Institute.Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; Approved standard.2nd Ed[S].Wayne:PA CLSI M38-A2,2008.
[9] Gupta P,Khare V,Kumar D,et al.Comparative evaluation of disc diffusion and E-test with broth micro-dilution in susceptibility testing of amphotericin B,voriconazole and caspofungin against clinical Aspergillus isolates[J].JClin Diagn Res,2015,9(1):DC04-DC07.
[10] Wang H,Lei Y,Yan L,et al.Deep sequencing analysis of transcriptomes in Aspergillus flavus in response to resveratrol[J].BMC Microbiology,2015,15(1):182.
[11] Adrian M,Jeandet P,Veneau J,et al.Biological activity of resveratrol,a stilbenic compound,from grapevines,against botrytis cinerea,the causal agent for gray mold[J].J Chem Ecol,1997,23(7):1689-1702.
[12] Bhatia VK,Sharma PC.Epidemiological studies on dermatophytosis in human patient in Himachal Pradesh India[J].Springerplus,2014,3(1):134.
[13] Gong JQ,Liu XQ,Xu HB,et al.Deep dermatophytosis caused by Trichophyton rubrum:Report of two cases[J].Mycoses,2007,50(2):102-108.
[14] Vandeputte P,Ferrari S,Coste AT.Antifungal resistance and new strategies to control fungal infection[J].Int J Microbiol,2012,2012(3):713687.
[15] 欧阳秋,黄 晓,陶 科,等.瑞香狼毒对石膏样毛癣菌的抑制作用及对超微结构的影响[J].华西药学杂志,2008,23(1):010-012.
Ouyang Q,Huang X,Tao K,et al.Fungistasis activity of Stellera chamaejasme L.and influence on the ultrastructure of Trichophyton mentagrophytes[J].West China Journal of Pharmaceutical Sciences,2008,23(1):010-012.
[16] 董晨虹,段秀俊,阴孟凡,等.凤仙花不同部位水提液抑真菌效果的实验研究[J].山西中医学院学报,2017,18(5):19-21.
Dong CH, Duan XJ, Yin MF,et al.Experimental study on antifungal effectof different parts of impatiens[J].Journal of Shanxi College of Trditional Chinese Medicine,2017,18(5):19-21.
[17] 谭翠雯,吴碧娟,孙婧雯,等.改良CLSI M38-A2应用于皮肤癣菌对甾体皂苷敏感性的测定[J].中成药,2017,39(4):828-830.
Tang CW,Wu BJ,Sun JW,et al.Modified CLSI M 38-A2 was used to determine the sensitivity of tinea to saponin[J].Chinese Traditional Patent Medicine,2017,39(4):828-830.
[18] 王 鑫,张 瑞,李雅静,等.白藜芦醇对红色毛癣菌和烟曲霉菌抑菌作用比较[J].现代检验医学杂志,2014,29(2):92-93,96.
Wang X,Zhang R,Li YJ,et al.Antibacterial effect comparison of resveratrol against Trichophyton rubrum and Aspergillus fumigatus[J].J Mod Lab Med,2014,29(2):92-93,96.

相似文献/References:

[1]王 鑫,张 瑞,李雅静,等.白藜芦醇对金黄色葡萄球菌标准株 抑菌作用及超微结构的影响[J].现代检验医学杂志,2016,31(03):55.[doi:10.3969/j.issn.1671-7414.2016.03.015]
　WANG Xin,ZHANG Rui,LI Ya-jing,et al.Antibacterial and Ultrastructure Effect of RES on Staphylococcus Aureus Standard Strains ATCC 25923[J].Journal of Modern Laboratory Medicine,2016,31(05):55.[doi:10.3969/j.issn.1671-7414.2016.03.015]
[2]王 鑫,张 瑞,李雅静,等.白藜芦醇对葡萄球菌标准株抑菌作用研究[J].现代检验医学杂志,2015,30(02):91.[doi:10.3969/j.issn.1671-7414.2015.02.028]
　WANG Xin,ZHANG Rui,LI Ya-jing,et al.Antibacterial Effect of Resveratrol on Staphylococcus Aureus Standard Strains[J].Journal of Modern Laboratory Medicine,2015,30(05):91.[doi:10.3969/j.issn.1671-7414.2015.02.028]

备注/Memo

备注/Memo:
作者简介:张 瑞(1984-),女,硕士,主管检验师,主要从事临床检验的研究,E-mail:lyjydeyjyk@163.com。
更新日期/Last Update: 2018-09-30