[1]金秀华,李艳冬.蒙古族儿童哮喘与TIM-1基因启动子区-416G>C,-1454G>A位点多态性和外显子4插入/缺失变异的相关性研究[J].现代检验医学杂志,2018,33(06):26-29.[doi:10.3969/j.issn.1671-7414.2018.06.007]
　JIN Xiu-hua,LI Yan-dong.Relationship between TIM-1 Gene Promoter Region -416G>C,-1454G>A,Exon 4(157insMTTTVP)Polymorphisms and Mongolian Nationality Childhood with Asthma[J].Journal of Modern Laboratory Medicine,2018,33(06):26-29.[doi:10.3969/j.issn.1671-7414.2018.06.007]
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蒙古族儿童哮喘与TIM-1基因启动子区-416G>C,-1454G>A位点多态性和外显子4插入/缺失变异的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年06期
页码:
26-29
栏目:
论著
出版日期:
2018-12-24

文章信息/Info

Title:
Relationship between TIM-1 Gene Promoter Region -416G>C,-1454G>A,Exon 4(157insMTTTVP)Polymorphisms and Mongolian Nationality Childhood with Asthma
文章编号:
1671-7414(2018)06-026-04
作者:
金秀华1; 李艳冬2
1.赤峰市蒙医中医医院检验科,内蒙古赤峰 024000; 2.赤峰市红山区妇幼保健所检验科,内蒙古赤峰 024000
Author(s):
JIN Xiu-hua1; LI Yan-dong2
Department of Clinical Laboratory,the Traditional Mongolian Medicine of Chinese Medicine Hospital of Chifeng City,Inner Mongolia Chifeng 024000, China; 2.Department of Clinical Laboratooory,Hongshan District Health Center for Women and Children of Chifeng City,Inner Mongolia Chifeng 024000,China
关键词:
儿童哮喘;  单核苷酸多态性;  T细胞免疫球蛋白黏蛋白域1(TIM-1)
分类号:
R725.6; Q503
DOI:
10.3969/j.issn.1671-7414.2018.06.007
文献标志码:
A
摘要:
目的 探讨内蒙古地区蒙古族儿童哮喘免疫调节基因T细胞免疫球蛋白黏蛋白域1(TIM-1)启动子区-416G>C,-1454G>A和外显子4插入/缺失变异与儿童哮喘的相关性。方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测方法,分别对蒙古族哮喘组儿童129例和对照组儿童130例检测Tim-1启动子区-416G>C,-1454G>A两个多态性位点和外显子4插入/缺失变异,进行病例对照研究分析。利用SPSS17.0对基因型/等位基因等计数资料采用Fisher χ2检验分析统计学差异。结果 哮喘组-416G>C位点基因多态性的C等位基因明显低于对照组,差异有统计学意义(OR=1.682,95%CI 1.146～2.268,P<0.05)。哮喘组TIM-1启动子区-1454G>A位点基因多态性与对照组比较(χ2=0.561,P=0.755)差异均无统计学意义; 哮喘组外显子4插入/缺失变异与对照组比较,差异均无统计学意义(χ2=1.013,P=0.602)。结论 Tim-1基因-416G>C位点与蒙古族儿童哮喘易感性相关。
Abstract:
Objective To explore whether the SNPs in TIM-1exon 4(157insMTTTVP)and two polymorphism loci,-416G>C,-1454G>A at promoter region were associated with childhood asthma in population in Inner Mongolia.Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to test the genotypes of three polymorphism loci among 129 cases of asthma and 130 controls in a case-control study.Using the count data of genotypes/alleles in SPSS17.0,Fisher χ2 test wasused to analyze the statistical differences.Results TIM-1 -416G>C polymorphism was associated with childhood asthma in population inInner Mongolia(OR=1.682,95%CI 1.146～2.268,P<0.05),but at promoterregion -1454G>A polymorphism(χ2=0.561,P=0.755)and exon 4(157insMTTTVP)at exon region(χ2=1.013,P=0.602)were not.Conclusion The results indicated that -416G>C polymorphism of the TIM-1 gene was associated with childhood asthma in population in Inner Mongolia.

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备注/Memo

备注/Memo:
作者简介:金秀华(1972-),女,本科,副主任检验师,检验科主任,主要从事哮喘基因多态性基础研究,E-mail:1813214327@qq.com。
更新日期/Last Update: 2018-11-30