[1]周俊英,田宏攀.临床血流感染沙门菌的分型及同源性和耐药性分析[J].现代检验医学杂志,2019,34(01):83-84,88.[doi:10.3969/j.issn.1671-7414.2019.01.021]
ZHOU Jun-ying,TIAN Hong-pan.Typing and Homology and Resistance Analysis of Salmonella
in Clinical Bloodstream Infection[J].Journal of Modern Laboratory Medicine,2019,34(01):83-84,88.[doi:10.3969/j.issn.1671-7414.2019.01.021]
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临床血流感染沙门菌的分型及同源性和耐药性分析(
)
《现代检验医学杂志》[ISSN:/CN:]
- 卷:
-
第34卷
- 期数:
-
2019年01期
- 页码:
-
83-84,88
- 栏目:
-
论著
- 出版日期:
-
2019-02-28
文章信息/Info
- Title:
-
Typing and Homology and Resistance Analysis of Salmonella
in Clinical Bloodstream Infection
- 文章编号:
-
1671-7414(2019)01-083-03
- 作者:
-
周俊英; 田宏攀
-
(武汉大学中南医院检验科，武汉430
071)
- Author(s):
-
ZHOU Jun-ying; TIAN Hong-pan
-
(Department of
Clinical Laboratory，Zhongnan Hospital of Wuhan University，Wuhan 430071，
China)
-
- 关键词:
-
沙门菌; 血清分型; 基因分型; 耐药性; 肠杆菌科基因间重复
序列的聚合酶链反应; 同源性分析
- 分类号:
-
378.22；R446.5
- DOI:
-
10.3969/j.issn.1671-7414.2019.01.021
- 文献标志码:
-
A
- 摘要:
-
目的探讨临床血液感染沙门菌血清分型、基因同源性及耐
药质粒和细菌耐药性的关系。方法收集武汉大学中南医院2015～20
17年临床血流感染分离的10株沙门菌。采用法国梅里埃Vitek 2 Compact全自动鉴定药敏检
测系统进行鉴定和药敏实验，按国家标准对沙门菌进行血清学分型，用肠杆菌科基因间重复
序列的聚合酶链反应(ERIC-PCR)对其进行基因分型，采用Cluster 3.0软件对PCR扩增产物
进行聚类分析。结果10株沙门菌分为3种血清群，A群1株，B群3株
，D群6株，D群是优势血清群，约占60.0%；10株沙门菌中2，3，4，5和8号菌株含有质粒，
不含质粒的是1，6，7，9和10号菌株；10株沙门菌对氨苄西林耐药率为80.0%，左氧氟沙星
中介率80.0%，对三、四代头孢及碳青霉烯类100.0%敏感，对复方磺胺类敏感率为80.0%
；4号和10号菌株、8号和9号菌株的同源性超过80.0%，1号和2号，5号和6号菌株的同源性
大于70.0%。结论沙门菌存在耐药质粒，质粒的多少表明耐药程度
的高低；沙门菌对氨苄西林及氨苄西林/舒巴坦耐药率较高，对头孢他啶敏感率高，因此治
疗血流感染时可选用三、四代头孢或亚胺培南，采用降阶梯治疗。
- Abstract:
-
Objective To investigate the relationship betwe
en serotyping，gene homology，drug-resistant plasmids and bacterial resistance
of Salmonella in clinical blood infection．Methods 10
strains of Salmonella isolated from clinical blood stream infection in Zhon
gnan Hospital of Wuhan University during the past 2015~2017 years were collecte
d．The strains identification and drug sensitive test were performed by French B
ioMerieux Vitek 2 Compact automatic identification and drug sensitivity detectio
n system，serological typing was carried out according to the national standard
，genotyping was conducted by enterobacterial repetitive intergenic consensus se
quence polymerase chain reaction (ERIC-PCR)，clustering analysis was done using
Cluster 3 software for PCR amplification products．Results”B
10 strains of Salmonella contained 3 serum groups，1 strain of A group，3
strains of B group，6 strains of group D respectively，D group was the dominant
serogroup，accounted for 60.0%，10 strains of Salmonella containing plasmi
d were strain 2，strain 3，strain 4，strain 5，strain 8，and no plasmid containi
ng bacteria strain 1，strain 6，strain 7，strain 9，strain 10，10 strains Salm
onella resistant rate to ampicillin were 80.0%，levofloxacin intermediary rat
e reached 80%，but their sensitive rates to the third or fourth generation cepha
losporins and carbapenems were 100.0%，their sensitive rate to compound sulfani
lamide was 80.0%．The gene homology between strain 4 and strain 10 was more tha
n 80.0%，strain 9 and strain 8 homology was also over 80.0%，the gene homology
between strain 1 and strain 2 and between strain 5 and strain 6 was over 70.0%
．Conclusion There were some plasmids on some Salmonell
a strains which implied the level of drug resistance． Salmonella resistanc
e rates to ampicillin and ampicillin/sulbactam were high，but they were very sen
sitive to ceftazidime，and so the treatment of bloodstream infection has better
to select the third or fourth generation ceftazidime or imipenem and employ dees
calation therapy strategy
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[7］刘芳萍，蔺月霞，张程程，等．鸡源性多重耐药沙门菌质粒与耐药性相关性分析［J
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备注/Memo
- 备注/Memo:
-
作者简介：
周俊英(1972-)，女，硕士，副主任技师，主要从事病原微生物学的临床与教学工
作，E-mail：1348548858@qq.com。
更新日期/Last Update:
2019-02-28