[1]刘春兴,高永辉,汤在祥.2型糖尿病患者血浆miR-144水平与吸烟状况的相关性研究[J].现代检验医学杂志,2019,34(02):17-19.[doi:10.3969/j.issn.1671-7414.2019.02.005]
　LIU Chun-xing,GAO Yong-hui,TANG Zai-xiang.Correlation between Plasma miR-144 Leveland Smoking Status in Patients with Type 2 Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2019,34(02):17-19.[doi:10.3969/j.issn.1671-7414.2019.02.005]
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2型糖尿病患者血浆miR-144水平与吸烟状况的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年02期
页码:
17-19
栏目:
论著
出版日期:
2019-05-11

文章信息/Info

Title:
Correlation between Plasma miR-144 Leveland Smoking Status in Patients with Type 2 Diabetes Mellitus
文章编号:
1671-7414(2019)02-017-03
作者:
刘春兴1; 高永辉1; 汤在祥2
(1.华东疗养院,江苏无锡 214065; 2.苏州大学医学部公共卫生学院,江苏苏州 215001)
Author(s):
LIU Chun-xing1; GAO Yong-hui1; TANG Zai-xiang2
(1.Huadong Sanatorium,JiangsuWuxi 214065,China; 2.School of Public Health,Soochow University,Jiangsu Suzhou 215001,China)
关键词:
2型糖尿病;  吸烟指数;  miR-144
分类号:
R587.1; R392.11
DOI:
10.3969/j.issn.1671-7414.2019.02.005
文献标志码:
A
摘要:
目的 探讨2型糖尿病(T2DM)患者血浆miR-144水平与吸烟的相关性。方法 在2018年1月～6月的体检人群中选择T2DM患者70例,按照吸烟情况分为不吸烟组31例、戒烟组13例和吸烟组26例。收集研究对象一般资料、饮酒史、吸烟史,检测空腹血葡萄糖(GLU)和糖化血红蛋白(HbA1c),采用定量反转录聚合酶链反应(qRT-PCR)检测血浆miR-144含量,分析三组人群的检测结果,评价吸烟指数与血浆miR-144的关系。结果 三组人群中年龄、饮酒史、BMI,GLU,HbA1c等比较,差异均无统计学意义(F=0.184,P=0.791; χ2=0.321,P=0.611; F=0.348,P=0.849; F=0.266,P=0.914; F=0.005,P=0.171); 吸烟组血浆miR-144水平(2.14×10-5±1.93×10-5nmol/L)高于不吸烟组(1.15×10-5±1.18×10-5nmol/L)(t=5.632,P=0.021),但与戒烟组差异无统计学意义(t=0.872 4,P=0.388 3); 戒烟组血浆miR-144水平(1.5×10-5±2.2×10-5nmol/L)与不吸烟组比较,差异无统计学意义(t=1.179,P=0.244 1); 吸烟指数与血浆miR-144水平呈正相关(r=0.915,P<0.001)。结论 吸烟导致T2DM患者血浆miR-144水平升高,血浆miR-144水平与吸烟指数呈正相关。
Abstract:
Objective To investigate the correlation between plasma miR-144 level and smoking in patients with type 2 diabetes mellitus(T2DM).Methods 70 patients with T2DM were selected from the physical examination population from January to June,2018,and divided into non-smoking group(31 cases),smoking cessation group(13 cases)and smoking group(26 cases)according to smoking status.General information,drinking historyand smoking history of the subjects were collected.Fasting blood glucose(GLU)and glycosylated hemoglobin(HbA1c)were detected.Plasma mir-144 content wasdetected by quantitative reverse transcription polymerase chain reaction(RT-PCR).Results There were no significant differencesin age,drinking history,BMI,GLU and HbA1c among the three groups(F=0.184,P=0.791; χ2=0.321,P=0.611; F=0.348,P=0.849; F=0.266,P=0.914; F=0.005,P=0.171).Plasma miR-144 level(2.14×10-5±1.93×10-5nmol/L)in smoking group washigher than that in non-smoking group(1.15×10-5±1.18×10-5nmol/L),(t=2.271,P=0.026 5).But there was no significant difference between smoking and smoking cessation groups(t=0.872 4,P=0.388 3).There wasalso no significant difference in plasma miR-144 between the smoking cessationgroup(1.5×10-5±2.2×10-5nmol/L)and the non-smoking group(t=1.179,P=0.2441).Smoking index was positively correlated with plasma miR-144 level(r=0.915,P<0.001).Conclusion Smoking increased the plasma miR-144 level in T2DM patients,and the plasma miR-144 level was positively correlated with smoking index.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81773541)。 作者简介:刘春兴(1979-),男,华东疗养院副主任技师,主要从事检验医学方面研究工作,E-mail:znso4liu@163.com。 通讯作者:汤在祥,男,苏州大学医学部公共卫生学院副教授,主要从事卫生统计学理论与方法,医疗大数据分析研究工作,E-mail: tangzx@suda.edu.cn。 收稿日期:2018-12-06 修回日期:2019-02-02
更新日期/Last Update: 2019-04-30