[1]夏 芳a,汪隆海a,方 超a,等.血清5种生物标志物水平联合检测对心力衰竭的诊断价值[J].现代检验医学杂志,2019,34(04):22-27.[doi:10.3969/j.issn.1671-7414.2019.04.006]
　XIA Fanga,WANG Long-haia,FANG Chaoa,et al.Combined Detection of Serum 5 Biomarkers for the Diagnostic Value of Heart Failure[J].Journal of Modern Laboratory Medicine,2019,34(04):22-27.[doi:10.3969/j.issn.1671-7414.2019.04.006]
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血清5种生物标志物水平联合检测对心力衰竭的诊断价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
22-27
栏目:
论著
出版日期:
2019-08-20

文章信息/Info

Title:
Combined Detection of Serum 5 Biomarkers for the Diagnostic Value of Heart Failure
文章编号:
1671-7414(2019)04-022-06
作者:
夏 芳1a; 汪隆海2a; 方 超1a; 张求霞2a; 黄 飞2a; 谷光敏1b; 王 勇2b; 程 刚1c; 宋 川2c
(1.安徽省含山县人民医院a.检验科; b.心内科; c.彩超室,安徽含山238100; 2.宋庆龄爱心医院a.检验科; b.心内科; c.彩超室,安徽巢湖 238000)
Author(s):
XIA Fang1a; WANG Long-hai2a; FANG Chao1a; ZHANG Qiu-xia2a; HUANG Fei2a;  GU Guang-min1b; WANG Yong2b; CHENG Gang1c; SONG Chuan2c
(1a.Department of Clinical Laboratory; 1b.Department ofCardiology; 1c.Room of Ultrasonic Diagnosis,the People's Hospital of Hanshan County,Anhui Hanshan 238100,China; 2a.Department of Clinical Laboratory; 2b.Department of Cardiology; 2c.Room of Ultrasonic Diagnosis,Song Qingling Caring Hospital,AnhuiChaohu 238000,China)
关键词:
生物标志物;  心力衰竭;  联合检测;  诊断价值
分类号:
R541.6; R392.11
DOI:
10.3969/j.issn.1671-7414.2019.04.006
文献标志码:
A
摘要:
目的 评价血清5种生物标志物水平对心力衰竭(HF)患者的诊断价值。方法 采用化学发光法与定量ELISA改进法检测不同程度HF患者的血清5种生物标志物水平,运用IBM SPSS23.0与SAS 9.4软件对结果进行统计处理。结果 Ⅰ～Ⅳ级HF患者的血清5种生物标志物水平明显增高,N末端脑钠肽(NT-proBNP)、中段心房利钠肽原(MR-proANP)、大内皮素1(BigET-1)、和肽素(copeptin)与半乳糖凝集素-3(Gal-3)的结果分别为3.09±0.37,3.53±0.55,3.70±0.52和3.93±0.46 ng/L,2.50±0.27,3.01±0.60,3.21±0.58和3.43±0.63pmol/L,3.97±1.34,5.43±1.80,6.14±1.80和7.09±1.98 pmol/L,16.12±3.19,19.22±3.74,21.30±3.86和23.54±4.42 pmol/L,29.94±4.06,34.35±5.68,36.58±5.90和39.60±6.35 μg/L,差异均具有统计学意义(F=71.804～128.045.均P<0.000)。5种生物标志物的实验受试者特征曲线下面积分别为0.988 5,0.986 6,0.949 2,0.978 3和0.977 4,它们两两比较,9对有差别,1对无差别。用NT-proBNP≥719.5 ng/L,MR-proANP≥192.5 pmol/L,Big ET-1≥3.45 pmol/L,和肽素≥14.0 pmol/L与Gal-3≥27.15 μg/L来联合诊断HF患者,有2项符合即可诊断。敏感度为98.6%,特异度为98.2%,诊断符合率为98.5%。结论 联合检测血清5种生物标志物,能提高HF诊断的敏感度和特异度。
Abstract:
Objective To evaluate the diagnostic value of serum 5 biomarkers in patients with heart failure(HF).Methods The serum levels of 5 biomarkers in HF patients with different degrees were detected by chemiluminescence and modified quantitative enzyme-linked immunosorbent assay.IBM SPSS23.0 and SAS9.4 software were used for statistical processing of the results.Results The serum levels of 5 biomarkers were significantly elevated in patients with class Ⅰ～Ⅳ HF.The resultsof N-terminal pro-brain natriuretic peptide,midregional pro-atrial natriuretic peptide,big endothelin-1,copeptin and galectin-3 were 3.09±0.37,3.53±0.55,3.70±0.52 and 3.93±0.46 ng/L; 2.50±0.27,3.01±0.60,3.21±0.58 and 3.43±0.63 pmol/L; 3.97±1.34,5.43±1.80,6.14±1.80 and 7.09±1.98 pmol/L; 16.12±3.19,19.22±3.74,21.30±3.86 and 23.54±4.42 pmol/L,29.94±4.06,34.35±5.68,36.58±5.90 and 39.60±6.35 μg/L,respectively.The differences were statistically significant(all P=0.000).Thearea under of receiver operator characteristic curve for 5 biomarkers were 0.988 5,0.986 6,0.949 2,0.978 3 and 0.977 4,respectively.When they were compared in pairs,nine pairs were different and one pair was no difference.HF patients were diagnosed with NT-proBNP≥719.5 ng/L,MR-proANP≥192.5 pmol/L,Big ET-1≥3.45 pmol/L,copeptin≥14.0 pmol/L and Gal-3≥27.15 μg/L,and two conformance could be diagnosed.The sensitivity,specificity and diagnostic coincidence rate were 98.6%,98.2% and 98.5% respectively with two results to diagnose HF.Conclusion Combined detection of 5 biomarkers in serum can improve the sensitivity and specificity of HF diagnosis.

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备注/Memo

备注/Memo:
作者简介:夏 芳(1978-),女,硕士研究生,副主任检验师,从事临床生物化学检验与研究,E-mail:xiayueye1978@163.com。收稿日期:2019-05-05 修回日期:2019-05-20
更新日期/Last Update: 2019-07-30