[1]刘宗萍.慢性乙肝患者血清 Tim-3，HBsAg，TGF-β1水平表达与病情程度相关性研究[J].现代检验医学杂志,2019,34(06):77-81.[doi:10.3969 ／ j.issn.1671-7414.2019.06.019]
　LIU Zong-ping.Study on the Correlation between the Expression of Serum Tim-3, HBsAg, TGF-beta 1 and the Severity of Chronic Hepatitis B[J].Journal of Modern Laboratory Medicine,2019,34(06):77-81.[doi:10.3969 ／ j.issn.1671-7414.2019.06.019]
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慢性乙肝患者血清 Tim-3，HBsAg，TGF-β1水平表达与病情程度相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年06期
页码:
77-81
栏目:
论著
出版日期:
2019-12-30

文章信息/Info

Title:
Study on the Correlation between the Expression of Serum Tim-3, HBsAg, TGF-beta 1 and the Severity of Chronic Hepatitis B
文章编号:
1671-7414（2019）06-077-05
作者:
刘宗萍
（陕西省宝鸡市口腔医院检验科　陕西宝鸡　721000）
Author(s):
LIU Zong-ping
（Department of Clinical Laboratory, Baoji Stomatological Hospital, Shaanxi Baoji 721000, China）
关键词:
T 淋巴细胞免疫球蛋白黏蛋白分子3; 乙肝表面抗原; 转化生长因子β1; 慢性乙型肝炎;  病情程度; 诊断价值
分类号:
R512.62；R392.11
DOI:
10.3969 ／ j.issn.1671-7414.2019.06.019
文献标志码:
A
摘要:
目的　探讨慢性乙肝患者血清T 淋巴细胞免疫球蛋白黏蛋白分子3（T lymphocyte immunoglobulin mucin molecule 3，Tim-3）、乙肝表面抗原（hepatitis B surface antigen，HBsAg）和转化生长因子β1（transforming growth factor beta 1，TGF-β1）表达与病情程度关联性。方法　选取宝鸡市口腔医院2017 年10 月~2019 年6 月诊断为慢性乙肝患者103 例为研究组，选择同期健康体检者103 例为对照组，两组患者均检测肝功能[ 肝胆酸（cholyglycine，CG）、谷氨酸 氨基转移酶（alanine aminotransferase，ALT）、天冬氨酸氨基转移酶（aspartate aminotransferase，AST）、直接胆红素 （direct bilirubin，DBIL）、总胆红素（total bilirubin，TBIL）]、TGF-β1，HBsAg 和Tim-3 表达情况，并分析慢性乙肝 发病影响因素、各指标水平与肝功能指标水平关联性、诊断效能。结果　①研究组TBIL，DBIL，CG，AST 和ALT 血 清水平高于对照组，差异均有统计学意义（t =24.412~53.782，均P ＜ 0.05）。②研究组Tim-3，TGF-β1 及HBsAg 水平 高于对照组，差异均有统计学意义（t =29.878~37.574，均P ＜ 0.05）。③ HBsAg（OR=2.234）、Tim-3（OR=2.634） 及TGF-β1（OR=2.768）均为影响慢性乙肝发病的独立危险因素（χ2=5.862~11.140，均P ＜ 0.05）。④ TGF-β1，Tim-3 及HBsAg 与肝功能指标CG，ALT，AST，DBIL，TBIL 存在显著正相关关系（均P ＜ 0.05）。⑤联合诊断敏感度 （95.15%）、特异度（92.23%）和准确度（93.69%）高于TGF-β1（79.61%，76.70% 和78.16%）、Tim-3（77.67%， 80.58% 和79.13%）及HBsAg（82.52%，83.50% 和83.01%）单独诊断，差异有统计学意义（χ2=9.662~22.685，均P ＜ 0.05）。 结论　慢性乙肝患者Tim-3，HBsAg 和TGF-β1 水平与肝功能存在正相关关系，通过联合检测可提升慢性乙肝诊断敏感 度及准确度、特异度。
Abstract:
Objective　To investigate the T lymphocyte immunoglobulin mucin molecule 3 (Tim-3), hepatitis B surface antigen (HBsAg) and transforming growth factor beta 1 (TGF-β1) in patients with chronic hepatitis B correlates with serum expression and disease severity. Methods　103 patients with chronic hepatitis B in the Baoji Stomatobjical Hospital from October 2017 to June 2019 were selected as the study group and 103 healthy people in the same period as the control group. The liver function [Cholyglycine (CG), Alanine aminotransferase (ALT), aspartate aminotransferase (AST) were measured. The expression of direct bilirubin (DBIL), total bilirubin (TBIL)], TGF-beta 1, HBsAg and Tim-3 were analyzed, and the influencing factors of chronic hepatitis B, the correlation between the levels of various indicators and the levels of liver function indicators, and the diagnostic efficacy were analyzed.Results　① The serum levels of TBIL, DBIL, CG, AST and ALT in the study group were higher than those in the control group (t = 24.412~53.782, all P < 0.05). ② The levels of Tim-3, TGF-beta 1 and HBsAg in the study group were higher than those in the control group (t = 29.878,~37.574, all P < 0.05). ③ HBsAg (OR=2.234), Tim-3 (OR=2.634) and TGF-β1 (OR=2.768) were independent risk factors for the onset of chronic hepatitis B (χ2=5.862~1.140, all P <0.05). ④ There was a significant positive correlation between TGF-beta 1, Tim-3 and HBsAg and liver function indicators CG, ALT, AST, DBIL and TBIL (P < 0.05). ⑤ The sensitivity (95.15%), specificity (92.23%) and accuracy (93.69%) of combined diagnosis were higher than those of TGF-beta 1 (79.61%, 76.70%, and 78.16%), Tim-3 (77.67%, 80.58%, and 79.13%) and HBsAg (82.52%, 83.50%, and 83.01%) respectively, with significant differences (χ2= 14.207, 9.662, 22.685,all P < 0.05).Conclusion　The level of Tim-3, HBsAg and TGF-1 in patients with chronic hepatitis B was positively related to the liver function. The sensitivity, accuracy and specificity of the diagnosis of chronic hepatitis B can be improved by combined detection.

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备注/Memo

备注/Memo:
作者简介：刘宗萍（1973-），女，主管检验师，主要从事临床医学检验相关工作，E-mail:35797727@qq.com。收稿日期：2019-08-11
更新日期/Last Update: 2019-12-25