[1]代鑫露,黄松音,李红玉.三种方法评估替加环素对多重耐药鲍曼不动杆菌体外药敏结果分析[J].现代检验医学杂志,2020,35(03):103-106.[doi:10.3969/j.issn.1671-7414.2020.03.027]
 DAI Xin-lu,HUANG Song-yin,LI Hong-yu.Comparision of Three Susceptibility Testing Methods of Tigecycline Against Multidrug Resistant Acinetobacter Baumannii[J].Journal of Modern Laboratory Medicine,2020,35(03):103-106.[doi:10.3969/j.issn.1671-7414.2020.03.027]
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三种方法评估替加环素对多重耐药鲍曼不动杆菌体外药敏结果分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年03期
页码:
103-106
栏目:
研究简报·实验技术
出版日期:
2020-07-20

文章信息/Info

Title:
Comparision of Three Susceptibility Testing Methods of Tigecycline Against Multidrug Resistant Acinetobacter Baumannii
文章编号:
1671-7414(2020)03-103-04
作者:
代鑫露黄松音李红玉
(中山大学孙逸仙纪念医院检验科,广州 510120)
Author(s):
DAI Xin-lu HUANG Song-yin LI Hong-yu
(Department of Clinical Laboratory, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University,Guangzhou 510120,China)
关键词:
替加环素鲍曼不动杆菌纸片扩散法MIC Test Strip 法(MTS 法)VITEK-2 法
分类号:
R378;R446.5
DOI:
10.3969/j.issn.1671-7414.2020.03.027
摘要:
目的 比较3 种药敏检测方法检测替加环素对多重耐药鲍曼不动杆菌敏感性的差异。方法 收集2018 年临床 分离耐碳青霉烯类鲍曼不动杆菌60 株,采用MIC Test Strip(MTS)法、VITEK-2 法、纸片扩散法分别检测替加环素对 耐碳青霉烯类鲍曼不动杆菌的敏感性。结果 按照FDA 标准,以MTS 法为参考,VITEK-2 法的一致率较高,纸片扩 散法的一致率较低。Vitek-2 法的MIC 值比MTS 法的MIC 值低1 ~ 2 个稀释度,利用MTS 法检测替加环素对耐碳青霉 烯鲍曼不动杆菌的耐药率达到23.3%,出现耐药株。结论 对于耐碳青霉烯类鲍曼不动杆菌,Vitek-2 法和纸片扩散法 均不适合检测替加环素的敏感性,不能作为常规方法,需用MTS 法确认。
Abstract:
Objective To study the difference of three testing methods on susceptibility results of tigecycline against multidrug resistant Acinetobacter baumannii (MDR-AB). Methods 60 carbapenem-resistant Acinetobacter baumannii (CRAB) strains were collected from Sun Yat-Sen Memorial Hospital. Minimum inhibitory concentration(MIC) and inhibitory zone diameters for tigecycline were determined by Vitek-2, MTS and disk diffusion methods . The results of Vitek-2 and disk diffusion methods were compared with MTS method . The differences with antimicrobial susceptibility among the three different methods were evaluated. Results According to FDA standard, the consistency rate of Vitek-2 method was higher than that of paper diffusion method and Vitek-2 result in MIC90 in 1 dilution was lower compared with MTS.The drug resistance rate of tegacycline to carbapenems resistant Acinetobacter baumannii was detected by MTS method, which reached 23.3%, and drug-resistant strains appeared. Conlusion For routine susceptibility testing of MDR-AB on tigecycline, the disk diffusion and Vitek-2 may not be proper because of the poor correlation of results between the MTS.

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备注/Memo

备注/Memo:
收稿日期:2019-03-17 修回日期:2020-02-17基金项目:广东省科技计划项目(项目编号2017A020215017)。 作者简介:代鑫露(1987-),女,大学本科,学士学位,主要研究方向为微生物及免疫,E-mail:443848397@qq.com
更新日期/Last Update: 2020-07-20