[1]翟励敏,王碧玉,初礼巍,等.广西桂林地区儿童9 种呼吸道病原体感染的检测结果分析[J].现代检验医学杂志,2020,35(05):147-150.[doi:10.3969/j.issn.1671-7414.2020.05.038]
 ZHAI Li-min,WANG Bi-yu,CHU Li-wei,et al.Study on the Results of Nine Respiratory Tract Pathogens Infection in Children in Guilin, Guangxi[J].Journal of Modern Laboratory Medicine,2020,35(05):147-150.[doi:10.3969/j.issn.1671-7414.2020.05.038]
点击复制

广西桂林地区儿童9 种呼吸道病原体感染的检测结果分析()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
147-150
栏目:
检验与临床
出版日期:
2020-11-01

文章信息/Info

Title:
Study on the Results of Nine Respiratory Tract Pathogens Infection in Children in Guilin, Guangxi
文章编号:
1671-7414(2020)05-147-04
作者:
翟励敏王碧玉初礼巍赵丽春
桂林市人民医院检验科,广西桂林 541002
Author(s):
ZHAI Li-min WANG Bi-yu CHU Li-wei ZHAO Li-chun
Department of Clinical Laboratory, the People’s Hospital of Guilin,Guangxi Guilin 541002, China
关键词:
呼吸道感染病原体间接免疫荧光
分类号:
R373.1;R392.11
DOI:
10.3969/j.issn.1671-7414.2020.05.038
文献标志码:
A
摘要:
目的 分析广西桂林地区呼吸道感染患儿病原体分布情况及流行病学特点,有助于临床医生对儿童呼吸道感染 疾病的诊治。方法 采集桂林市人民医院2018 年9 月~2019 年8 月1 737 例因呼吸道感染住院患儿血清标本,采用间 接免疫荧光法(IFA)联合检测血清中呼吸道九种病原体IgM 抗体,包括:嗜肺军团菌-IgM(LP-IgM)、肺炎支原体-IgM (MP-IgM)、Q 热立克次体-IgM(COX-IgM)、肺炎衣原体-IgM(CP-IgM)、腺病毒-IgM(ADV-IgM)、呼吸道合 胞病毒-IgM(RSV-IgM)、甲型流感病毒-IgM(IFA-IgM)、乙型流感病毒-IgM(IFB-IgM)及副流感病毒-IgM(PIV-IgM), 比较各种病原体在不同年龄段、月份、性别的分布情况,对病原体感染特点进行统计学分析和卡方检验。结果 在检 测的1 737 例患儿中,总阳性率为45.94%,MP-IgM 阳性率最高为30.74%;各年龄组间比较,其中IFB-IgM,RSV-IgM 和MP-IgM 阳性检出率差异均有统计学意义(χ2=26.033~120.807,均P <0.001),MP-IgM 呈现患儿年龄越大阳性检出 率越高的趋势,而RSV-IgM 呈现患儿年龄越小阳性检出率越高的趋势;性别间比较,其中ADV-IgM,IFB-IgM 和MPIgM 阳性检出率差异均有统计学意义(χ2=4.862 ~ 35.759,均P <0.05),女性患儿阳性率均比男性患儿高。结论 桂 林地区儿童九种呼吸道病原体感染以MP 为主,并且病原体感染均具有年龄和性别分布的特点。
Abstract:
Objective To analysis the clinical distribution and epidemiological characteristics respiratory tract pathogen infection in children in Guilin Area,so as to help the clinical diagnosis and treatment of respiratory tract infection in children. Methods Serum samples of 1737 hospitalized children with respiratory tract infection from September 2018 to August 2019 in Guilin People's Hospital were collected . They were detected for the IgM antibodies of nine respiratory tract pathogens ,including Legionella pneumophila(LP-IgM),Mycoplasma pneumophila(MP-IgM),Coxiellaburneti(COX-IgM),Chlamydia pneumophila(CPIgM), adenovirus(ADV-IgM),respiratory syncytial virus(RSV-IgM),influenza A virus(IFA-IgM),influenza B(IFB-IgM) and parainfluenza virus(PIV-IgM) by indirect immunofluorescence assay.Comparing the distribution of children with age, months and gender,the results were adopted for statistical analysis. Results Among the 1 737 child patients, the total positive rate was 45.94%,and the highest positive rate was found in MP-IgM. Comparison among age groups,the positive rates of IFB IgM, RSV IgM and MP IgM were significantly different (χ2 = 26.033~120.807, P< 0.001).MP IgM showed that the older the children were, the higher the positive rate was, while RSV IgM showed that the younger the children were, the higher the positive rate was, but RSV-IgM was the opposite. Compared the girls and boys, ADV-IgM,IFB-IgM and MP-IgM had statistical significant (χ2=4.862~35.759,P<0.05), girls positive detection rate were higher than boys. Conclusion MP are the main respiratory tract pathogens in children in Guilin, and the infection is related to months,ageand gender.

参考文献/References:

[1]  ZHAO Yanjie, LU Roujian, SHEN Jun, et al. Comparison of viral and epidemiological profiles of hospitalized children with severe acute respiratory infection in Beijing and Shanghai,China[J]. BMC Infectious Diseases, 2019, 19(1): 729.
[2]  陈华俊.小儿急性呼吸道感染9 项病原体的感染 情况及其分布特点[J]. 广西医学, 2018, 40(11): 1185-1188, 1199.    CHEN Huajun. Infection and distribution features of nine pathogens of acute respiratory tract infection in children [J]. Guangxi Medical Journal,2018,40(11): 1185-1188, 1199.
[3]  王丽,周光,王磊利,等.2013~2016 年14 383 例 呼吸道感染患者9 种病原体IgM 抗体检测结果分 析[J]. 中华医院感染学杂志, 2018, 28(17): 2579- 2582.    WANG Li, ZHOU Guang, WANG Leili, et al. IgM antibody test for 9 species of pathogens isolated from 14 383 patients with respiratory tract infections in 2013~2016 [J]. Chinese Journal of Nosocomiolo gy,2018,28(17):2579-2582.
[4]  余成强,王启明,何家花,等.陕西省商洛市儿童 呼吸道感染病原体血清流行病学研究[J].现代检验 医学杂志,2019, 34(6): 142-144.    YU Chengqiang, WANG Qiming, HE Jiahua,et al. Seroepidemiological study on pathogens of respiratory tract infection in children in Shangluo, Shaanxi [J]. Journal of Modern Laboratory Medicine,2019, 34(6): 142-144.
[5]  马联. 1 510 例九项呼吸道感染病原体检测结果分析 [J]. 医学检验与临床, 2018, 29(6):46-48.    MA Lian.Analysis of 1 510 cases of nine pathogen detection results of respiratory tract infection[J]. Medical Laboratory Science and Clinics, 2018, 29(6):46-48.
[6]  郑辉,彭亮,卓广超.儿童呼吸道感染病原体IgM 抗体检测结果分析[J].中华医院感染学杂志, 2015, 25(1): 236-237.    ZHENG Hui, PENG Liang, ZHUO Guangchao, et al. Detection of IgM antibodies in pathogens causing respiratory tract infections in children [J]. Chinese Journal of Nosocomiology, 2015, 25(1):236-237.
[7]  解娟, 张梦瑶, 李小侠, 等. 九项呼吸道病原体IgM 抗体联合检测对儿童呼吸道感染的临床意义[J]. 现 代检验医学杂志,2016, 31(4): 110-112, 116.    XIE Juan, ZHANG Mengyao, LI Xiaoxia, et al. Clinical significance of detecting immunoglobulin-M of nine pathogens in serum of children with respiratory tract infection [J]. Journal of Modern Laboratory Medicine, 2016, 31(4): 110-112,116.
[8]  赖科峰,连距飞,陈柳青,等.广州地区12 359 例 急性呼吸道感染儿童呼吸道9 项病原体检测结果分 析[J]. 中国妇幼保健, 2018, 33(13): 2996-2999.    LAI Kefeng, LIAN Jufei, CHEN Liuqing , et al. Analysis of 12 359 cases of 9 pathogens in children with acute respiratory infection in Guangzhou[J]. Maternal & Child Health Care of China, 2018, 33(13): 2996-2999.
[9]  何成禄,徐从琼,王玉明,等.呼吸道九联检IgM 检测对儿童急性下呼吸道感染的早期诊断价值[J]. 昆明医科大学学报, 2014, 35(11): 129-132.    HE Chenglu,XU Congqiong,WANG Yuming , et al. Value of nine respiratory pathogens detection in early diagnosis of children with acute lower respiratory tract infections [J].Journal of Kunming Medical University, 2014, 35(11): 129-132.
[10] 王群,欧维琳,宋晓丹,等.喘息性疾病患儿血清 九种呼吸道病原体IgM 抗体检测研究[J].中国医 药导报, 2013, 10(23): 46-48.    WANG Qun, OU Weilin, SONG Xiaodan,et al. Study on detection of IgM antibodies of 9 types of respiratory pathogen infection in children with asthmatic diseases [J]. China Medical Herald, 2013, 10(23): 46-48.
[11] 张新星,季伟,顾文婧,等.2005 年至2014 年苏 州地区儿童呼吸道肺炎支原体感染流行病学分析 [J]. 中华传染病杂志, 2015, 33(10): 594-598.    ZHANG Xinxing, JI Wei, GU Wenjing, et al. Epidemiological analysis of Mycoplasma pneumoniae infection in children with respiratory tract diseases in Suzhou area from 2005 to 2014 [J]. Chinese Journal of Infectious Diseases, 2015, 33(10): 594-598.
[12] MATSUDA K, NARITA M, SERA N, et al. Gene and cytokine profile analysis of macrolide-resistant Mycoplasma pneumoniae infection in Fukuoka, Japan [J]. BMC Infectious Diseases, 2013, 13(1): 591.
[13] 张海邻,陈志敏,刘瀚旻,等.肺炎支原体感染的 致病机制[J]. 中华儿科杂志, 2016, 54(2): 94-97.    ZHANG Hailin, CHEN Zhimin, LIU Hanmin, et al.Pathogenesis of Mycoplasma pneumoniae infection[J]. Chinese Journal of Pediatrics, 2016,54 (2):94-97.
[14] 姚娟,沈国松,范丽红,等.6 089 例住院儿童呼 吸道感染常见病毒病原学检测分析[J].中华流行病 学杂志,2015,36(6):664-666.    YAO Juan, SHEN Guosong, FAN Lihong, et al. Analysis on common virus pathogens in 6089 hospitalized children with respiratory tract infection[J]. Chinese Journal of Epidemiology,2015, 36(6): 664-666.
[15] 邱胜丰,葛高霞,张宪伟,等.儿童9 种呼吸道病 原体的IgM 抗体检测结果分析[J].中国卫生检验 杂志, 2015, 25(23): 4082-4084.    QIU Shengfeng, GE Gaoxia, ZHANG Xianwei, et al. A clinical analysis of 9 pathogenic IgM antibodies in children with respiratory tract infection [J]. Chinese Journal of Health Laboratory Technology,2015, 25(23): 4082-4084.
[16] 白洁,李培奋,何灵,等.9 项呼吸道病原体血清 IgM 抗体检测在呼吸道感染中的临床价值[J].国际 检验医学杂志, 2016, 37(18): 2573-2575.    BAI Jie, LI Peifen, HE Ling, et al. The value of IgM antibody with 9 respiratory pathogen in serum to respiratory tract infection [J]. International Journal of Laboratory Medicine, 2016, 37(18): 2573-2575.

相似文献/References:

[1]解 娟,张梦瑶,李小侠,等.九项呼吸道病原体IgM抗体联合检测对儿童呼吸道感染的临床意义[J].现代检验医学杂志,2016,31(04):110.[doi:10.3969/j.issn.16717-414.2016.04.031]
 XIE Juan,ZHANG Meng-yao,LI Xiao-xia,et al.Clinical Significance of Detecting Immunoglobulin-M of Nine Pathogens in Serum of Children with Respiratory Tract Infection[J].Journal of Modern Laboratory Medicine,2016,31(05):110.[doi:10.3969/j.issn.16717-414.2016.04.031]
[2]靳庆娥,苏建荣,乌姗娜,等.2015年北京地区成人急性呼吸道感染9种病原体IgM抗体检测分析[J].现代检验医学杂志,2017,32(02):157.[doi:10.3969/j.issn.1671-7414.2017.02.044]
 JIN Qing-e,SU Jian-rong,WU Shan-na,et al.Analysis of IgM Antibody of Nine Acute Respiratory Tract Infection Pathogens in Adults During 2015 in Beijing Area[J].Journal of Modern Laboratory Medicine,2017,32(05):157.[doi:10.3969/j.issn.1671-7414.2017.02.044]
[3]罗金秀a,邹海珊b,祝少凤b,等.广东清远地区儿童呼吸道感染患者三种病毒RNA 检测结果分析[J].现代检验医学杂志,2020,35(02):132.[doi:10.3969/j.issn.1671-7414.2020.02.036]
 LUO Jin-xiua,ZOU Hai-shanb,ZHU Shao-fengb,et al.Analysis of the Results for Detection of Children with Respiratory Infectionof Three Viral RNA in Guangdong Qingyuan District[J].Journal of Modern Laboratory Medicine,2020,35(05):132.[doi:10.3969/j.issn.1671-7414.2020.02.036]
[4]许笑雷,葛胜旺,陈春宁,等.石家庄地区儿童呼吸道病毒感染病原学及流行特征分析[J].现代检验医学杂志,2021,36(02):140.[doi:doi:10.3969/j.issn.1671-7414.2021.02.033]
 XU Xiao-lei,GE Sheng-wang,CHEN Chun-ning,et al.Analysis of Etiology and Epidemic Characteristics of Children Respiratory Tract Virus Infection in Shijiazhuang Area[J].Journal of Modern Laboratory Medicine,2021,36(05):140.[doi:doi:10.3969/j.issn.1671-7414.2021.02.033]
[5]唐月明,伊 洁.数字聚合酶链反应(dPCR)技术在病原体基因检测应用中的研究进展[J].现代检验医学杂志,2021,36(05):174.[doi:10.3969/j.issn.1671-7414.2021.05.038]
 TANG Yue-ming,YI Jie.Recent Advances in Research on Digital Polymerase ChainReaction(dPCR) in Pathogen Gene Detection[J].Journal of Modern Laboratory Medicine,2021,36(05):174.[doi:10.3969/j.issn.1671-7414.2021.05.038]
[6]康 茹,孟改丽,周雪红.呼吸道感染患儿肠道菌群紊乱与Th17/Treg及其分泌炎性细胞因子免疫平衡的相关性研究[J].现代检验医学杂志,2021,36(06):95.[doi:10.3969/j.issn.1671-7414.2021.06.020]
 KANG Ru,MENG Gai-li,ZHOU Xue-hong.Correlation Analysis between Intestinal Flora Disturbance and Immune Balance of Th17/Treg and Its Secretion of Inflammatory Cytokines Children with Respiratory Tract Infection[J].Journal of Modern Laboratory Medicine,2021,36(05):95.[doi:10.3969/j.issn.1671-7414.2021.06.020]
[7]李青松,赵聪平,刘 静,等.CRISPR-Cas13a 系统在病原体检测应用中的最新研究进展[J].现代检验医学杂志,2024,39(03):199.[doi:10.3969/j.issn.1671-7414.2024.03.034]
 LI Qingsong,ZHAO Congping,LIU Jing,et al.Recent Advances in the Application of CRISPR-Cas13a Systems in Pathogen Detection[J].Journal of Modern Laboratory Medicine,2024,39(05):199.[doi:10.3969/j.issn.1671-7414.2024.03.034]
[8]余成强,王启明,何家花,等.陕西省商洛市儿童呼吸道感染病原体血清流行病学研究[J].现代检验医学杂志,2019,34(06):142.[doi:10.3969 / j.issn.1671-7414.2019.06.036]
 YU Cheng-qiang,WANG Qi-ming,HE Jia-hua,et al.Seroepidemiological Study on Pathogens of Respiratory Tract Infection in Children in Shangluo, Shaanxi[J].Journal of Modern Laboratory Medicine,2019,34(05):142.[doi:10.3969 / j.issn.1671-7414.2019.06.036]

备注/Memo

备注/Memo:
基金项目:广西壮族自治区卫生厅自筹经费科研课题(编号Z20190453)。
作者简介:翟励敏(1988-),女,硕士研究生,主管检验技师,从事免疫及分子检验工作,E-mail:513405756@qq.com。
通讯作者:王碧玉(1970-),女,大学本科,副主任技师,从事临床免疫检验工作,E-mail:wby1225@126.com。
更新日期/Last Update: 2020-10-30