[1]刘国振,陈秀余,张修欢.血清 miR-122-5p，miR-486-5p，AFP及 AFP-L3水平联合检测对原发性肝癌的诊断价值[J].现代检验医学杂志,2022,37(01):82-87.[doi:10.3969/j.issn.1671-7414.2022.01.017]
　LIU Guo-zhen,CHEN Xiu-yu,ZHANG Xiu-huan.Value of Combined Detection of Serum miR-122-5p, miR-486-5p, AFP and AFP-L3 Levels in the Diagnosis of Primary LiverCancer[J].Journal of Modern Laboratory Medicine,2022,37(01):82-87.[doi:10.3969/j.issn.1671-7414.2022.01.017]
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血清 miR-122-5p，miR-486-5p，AFP及 AFP-L3水平联合检测对原发性肝癌的诊断价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年01期
页码:
82-87
栏目:
论　著
出版日期:
2022-01-15

文章信息/Info

Title:
Value of Combined Detection of Serum miR-122-5p, miR-486-5p, AFP and AFP-L3 Levels in the Diagnosis of Primary LiverCancer
文章编号:
1671-7414（2022）01-082-06
作者:
刘国振; 陈秀余; 张修欢
（安徽医科大学附属六安医院/ 六安市人民医院核医学科 , 安徽六安 237005)
Author(s):
LIU Guo-zhen;  CHEN Xiu-yu;  ZHANG Xiu-huan
（Department of Nuclear Medicine, Lu’an Hospital, Anhui Medical University/Lu’an People’s Hospital, Anhui Lu’an 237005 , China）
关键词:
血清 miR-122-5p; 血清 miR-486-5p; 甲胎蛋白; 甲胎蛋白异质体; 原发性肝癌
分类号:
R735.7；R730.43
DOI:
10.3969/j.issn.1671-7414.2022.01.017
文献标志码:
A
摘要:
目的　探讨血清 miR-122-5p，miR-486-5p，甲胎蛋白（ alpha fetoprotein,AFP）和甲胎蛋白异质体（ alpha-fetoprotein isosomes,AFP-L3）联合检测对原发性肝癌（ primary carcinoma）的诊断价值。方法　收集 2017年 2月～ 2020年 5月六安市人民医院原发性肝癌患者（ n=100）作为观察组，同时间六安市人民医院入院治疗良性肝脏病患者（ n=88）作为对照组 ,同时收集同期入院健康体检者 (n=80)为空白对照组。测定各组血清 miR-122-5p，miR-486-5p，AFP及 AFP-L3的表达水平；分析不同病理下 miR-122-5p，miR-486-5p，AFP及 AFP-L3水平；分析 miR-122-5p，miR-486-5p，AFP和 AFP-L3水平及其联合检测在原发性肝癌患者中的诊断价值。结果　观察组血清 AFP及 AFP-L3水平高于空白对照组及对照组，差异有统计学意义（ F=34.253，16.845，均 P=0.000）；对照组 AFP及 AFP-L3水平高于空白对照组，观察组血清 miR-122-5p和 miR-486-5p水平低于空白对照组及对照组，差异有统计学意义（ F=9.532，11.516，均 P=0.000）；对照组 miR-122-5p和 miR-486-5p水平低于空白对照组，差异均有统计学意义（ P＜ 0.05）。观察组不同病理下血清 miR-122-5p，miR-486-5p，AFP及 AFP-L3水平比较发现，在性别、年龄及病灶数目上差异均无统计学意义（ t=-1.711~0.434，均 P＞ 0.05），在肿瘤直径、 TNM分期、门静脉癌栓、脏器转移及淋巴结转移上差异有统计学意义（t=-7.132~12.485，P＜ 0.05）。血清 miR-122-5p，miR-486-5p，AFP及 AFP-L3联合检测其敏感度和特异度分别为
Abstract:
Objective　To explore the effects of combined detection of serum miR-122-5p, miR-486-5p, alpha fetoprotein (AFP),and alpha-fetoprotein isosomes (AFP-L3) on primary the diagnostic value of liver cancer. Methods Patients with primary livercancer (n=100) from Lu’an People’s Hospital were collected as the observation group, and patients with benign liver disease(n=88) admitted to Lu’an People’s Hospital were collected as the control group. At the same time, patients who were admitted tothe hospital for physical examination (n= 80) was the blank control group. Measured the expression levels of serum miR-122-5p,miR-486-5p, AFP and AFP-L3 in each group. Analyzed the levels of miR-122-5p, miR-486-5p, AFP and AFP-L3 under differentpathologies and analyzed miR the diagnostic value of -122-5p, miR-486-5p, AFP, AFP-L3 levels and their combined detection inpatients with primary liver cancer. Results　The levels of serum AFP and AFP-L3 in the observation group were higher thanthose in the blank control group and the control group, the differences between groups were statistically significant(F=34.253,16.845, all P=0.000), the levels of AFP and AFP-L3 in the control group were higher than those in the blank control group. Theserum levels of miR-122-5p and miR-486-5p in the observation group were lower than those in the blank control group. and thecontrol group, the differences between groups were statistically significant(F=9.532, 11.516, all P = 0.000 1), the levels of miR-122-5p and miR-486-5p in the control group were lower than the blank control group, and the differences were statisticallysignificant (P<0.05). Comparison of serum miR-122-5p, miR-486-5p, AFP and AFP-L3 levels in the observation group underdifferent pathologies found that there were no statistically significant differences in gender, age, and number of lesions (t=-1.711~0.434, all P> 0.05), there were statistical differences in tumor diameter, TNM stage, portal vein tumor thrombus, organmetastasis and lymph node metastasis (t=-7.132~12.485, P<0.05). The sensitivity and specificity of serum miR-122-5p, miR-486-5p, AFP and AFP-L3 combined detection were 92.37% and 90.45% respectively, AUC was 0.945, which was significantlyhigher than miR-122-5p, miR-486- 5p, single detection of AFP and AFP-L3, the difference was statistically significant (χ2=8.165,P ＜ 0.05). Conclusion　The combined detection of serum miR-122-5p, miR-486-5p, AFP and AFP-L3 can improve thediagnostic value of primary liver cancer, all of which are involved in the pathogenesis of primary liver cancer, and may be used todiagnose primary liver cancer. The effective index is worthy of clinical promotion.

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备注/Memo

备注/Memo:
作者简介：刘国振（1983-），男，本科，主管技师，研究方向：肺癌诊断治疗，E-mail:ly11975042322@163.com。
更新日期/Last Update: 1900-01-01