[1]王新庄,孟建涛.慢性心力衰竭患者血清 lncRNA MALAT1的表达水平及其临床意义[J].现代检验医学杂志,2022,37(01):141-144.[doi:10.3969/j.issn.1671-7414.2022.01.028]
 WANG Xin-zhuang,MENG Jian-tao.Expression of lncRNA MALAT1 in Peripheral Blood of Patients with Chronic Heart Failure and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2022,37(01):141-144.[doi:10.3969/j.issn.1671-7414.2022.01.028]
点击复制

慢性心力衰竭患者血清 lncRNA MALAT1的表达水平及其临床意义()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年01期
页码:
141-144
栏目:
论 著
出版日期:
2022-01-15

文章信息/Info

Title:
Expression of lncRNA MALAT1 in Peripheral Blood of Patients with Chronic Heart Failure and Its Clinical Significance
文章编号:
1671-7414(2022)01-141-04
作者:
王新庄孟建涛
( 西安高新医院急诊科,西安 710077)
Author(s):
WANG Xin-zhuang MENG Jian-tao
(Department of Emergency, Xi’an Gaoxin Hospital, Xi’an 710077, China)
关键词:
慢性心力衰竭长链非编码 RNA肺癌转移相关转录本 1生物学标志物
分类号:
R541.6;R392.11
DOI:
10.3969/j.issn.1671-7414.2022.01.028
文献标志码:
A
摘要:
目的 探究慢性心力衰竭 (chronic heart failure, CHF)患者血清长链非编码 RNA(long non-coding RNA, lncRNA)肺癌转移相关转录本 1(MALAT1)的表达水平及其临床意义。方法 选取 2017年 2月~ 2019年 5月西安高新医院急诊科收治的 62例 CHF患者为 CHF组,另选取同期 70例体检健康者为对照组。采用实时荧光定量 PCR(qRT-PCR)检测血清 lncRNA MALAT1的表达水平。采用酶联免疫吸附法 (ELISA)测定血清凋亡分子的水平。采用彩色多普勒超声仪检查并测定心力衰竭相关指标。相关性采用 Pearson相关性分析,采用受试者工作特征 (receiver operating characteristic, ROC)曲线评价 lncRNA MALAT1对 CHF的诊断效能。结果 CHF组患者血清 lncRNA MALAT1和 B淋巴细胞瘤 -2(B-cell lymphoma-2, Bcl-2)的水平均低于对照组, CHF组患者血清 Bcl-2相关 x蛋白 (BCL2-Associated X, Bax)水平高于对照组,差异均具有统计学意义 (t=16.31, 11.90, 9.57,均 P=0.00)。CHF组中美国纽约心脏病学会 (New York Heart Disease Assocation, NYHA)分级Ⅲ级和Ⅳ级患者血清 lncRNA MALAT1和 Bcl-2的水平低于 NYHAⅡ级, CHF组中 NYHA Ⅲ级和Ⅳ级患者血清 Bax水平高于 NYHAⅡ级, NYHAⅣ级患者血清 lncRNA MALAT1和 Bcl-2的水平低于Ⅲ级,血清 Bax水平高于Ⅲ级,差异均具有统计学意义 (均 P< 0.05)。CHF组患者左室射血分数 (left ventricular ejection fraction, LVEF)低于对照组,左室舒张末期内径 (left ventricular end diastolic diameter, LVEDD)和左室收缩末期内径 (left ventricular end systolic diameter, LVESD)高于对照组,差异均具有统计学意义 (t=4.56~10.85,均 P=0.00)。Pearson相关性分析显示, CHF组患者血清 lncRNA MALAT1和 Bcl-2水平与 LVEF呈正相关 (r=0.29,0.32,均 P< 0.05),与 LVEDD和 LVESD呈负相关 (r=-0.25,-0.31,P=0.01)。CHF组患者血清 Bax与 LVEF呈负相关 (r=-0.33,P=0.01),与 LVEDD和 LVESD呈正相关 (r=0.20,0.32,P=0.00)。CHF组患者血清 lncRNA MALAT1与 Bcl-2水平呈正相关 (r=0.31, P=0.00),与血清 Bax水平呈负相关 (r=-0.24,P=0.01)。血清 lncRNA MALAT1诊断 CHF的曲线下面积 (area under the curve, AUC)为 0.791,敏感度和特异度分别为 75.0%和 79.5% (95%CI=0.653~0.930,P=0.001)。结论 CHF患者血清 lncRNA MALAT1水平降低,其可作为评价 CHF病情发展的潜在生物学标志物。
Abstract:
Objective To explore the expression level of long non-coding RNA (lncRNA) MALAT1 in peripheral blood ofpatients with chronic heart failure (CHF) and its clinical significance. Methods 62 CHF patients admitted to the Department ofEmergency of Xi ’an Gaoxin Hospital from February 2017 to May 2019 were selected as the CHF group, and 70 healthy subjectsduring the same period were selected as the control group. The expression level of lncRNA MALAT1 in serum was detected byreal-time quantitative fluorescence PCR(qRT-PCR). The levels of apoptotic molecules in serum were determined by enzyme-linkedimmunosorbent assay (ELISA). Color Doppler ultrasonography was used to examine and determine the related indexes of heartfailure. The correlation was analyzed by Pearson correlation, receiver operating characteristic (ROC) curve was used to evaluatethe diagnostic efficacy of lncRNA MALAT1 for CHF. Results The serum lncRNA malAT1 and B-cell lymphoma-2 (Bcl-2) levelsin CHF group were lower than those in the control group, and the serum Bcl2-associated X protein (Bax) levels in CHF group werehigher than those in the control group, the difference were statistically significant (t=16.31, 11.90, 9.57, all P=0.00). Patients withCHF group New York Heart Disease Assocation (NYHA) Ⅲ and Ⅳ serum lncRNA MALAT1 and Bcl-2 levels below theNYHA Ⅱ level, in CHF group NYHA Ⅲ and Ⅳ patients serum Bax levels higher than the NYHA level, NYHA Ⅳ Ⅱlevel in patients with serum lncRNA MALAT1 and Bcl-2 levels below Ⅲ level, the serum Bax level was higher than Ⅲ level,and the difference wars statistically significant (all P<0.05). The left ventricular ejection fraction (LVEF) in CHF group werelower than that in control group, the left ventricular end diastolic diameter (LVEDD) and left ventricular end systolic diameter(LVESD) were higher than those in the control group, the difference were statistically significant (t=4.56~10.85, all P=0.00).Pearson correlation analysis showed that serum lncRNA MALAT1 and Bcl-2 levels in CHF group were positively correlated withLVEF (r=0.29, 0.32, all P<0.05), while negatively correlated with LVEDD and LVESD (r=-0.25, -0.31, P=0.01). Serum Bax inCHF group was negatively correlated with LVEF (r=-0.33, P=0.01), while positively correlated with LVEDD and LVESD(r=0.20, 0.32, all P=0.00). In CHF group, serum lncRNA MALAT1 was positively correlated with the level of Bcl-2 (r=0.31,P=0.00), and negatively correlated with the level of serum Bax (r=-0.24, P=0.01). The area under the curve (AUC) of serumlncRNA MALAT1 in the diagnosis of CHF was 0.791, the sensitivity and the specificity were 75% and 79.5%,respectively(95%CI=0.6533~0.930, P=0.001). Conclusion The serum lncRNA MALAT1 level was decreased in CHF patients, which canbe used as a potential biomarker to evaluate the disease progression of CHF.

参考文献/References:

[1] 苏虹,李仕林,王昌育,等.成纤维细胞生长因子23基因多态性与慢性心力衰竭患者预后的相关性研究[J].现代检验医学杂志,2020,35(1):35-39.SU Hong, LI Shilin, WANG Changyu, et al.Association of fibroblast growth factors 23 genepolymorphism and prognosis in patients with chronicheart failure[J]. Journal of Modern LaboratoryMedicine,2020,35(1):35-39.
[2] 崔贞,刘文.慢性心力衰竭患者呋塞米治疗对血清NT-proBNP 水平的影响[J].现代检验医学杂志,2019, 34(3):144-147.CUI Zhen, LIU Wen. Effects of furosemide in patientswith chronic heart failure on serum NT-proBNP[J]. Journal of Modern Laboratory Medicine, 2019,34(3):144-147.
[3] 唐红秀, 王璐, 万楠, 等. 慢性心力衰竭患者外周血RDW 与HDL-C 水平的相关性分析[J]. 现代检验医学杂志,2018,33(6):88-90,94.TANG Hongxiu, WANG Lu, WAN Nan, et al.Relationship between high-density lipoproteincholesterol and the red cell distribution width inpatients with chronic heart failure [J]. Journal ofModern Laboratory Medicine,2018,33(6):88-90,94.
[4] 张学正,李静,王琼,等.老年慢性心力衰竭患者运动康复的研究进展[J].医学综述,2020,26(10):1973-1977.ZHANG Xuezheng, LI Jing, WANG Qiong, et al.Research progress in exercise rehabilitation of senilepatients with chronic heart failure [J]. Medical Recapitulate,2020,26(10):1973-1977.
[5] NOBRE T S, ANTUNES-CORREA L M, GROEHSR V, et al. Exercise training improves neurovascularcontrol and calcium cycling gene expression in patientswith heart failure with cardiac resynchronizationtherapy[J]. American Journal of Physiology-Heart andCirculatory Physiology, 2016, 311(5): H1180-H1188.
[6] 张宝玺,刁海丹,李江宁.lncRNA MALAT1 和miR-200c 在卵巢癌中的表达关系及临床意义[J].河北医药,2019,41(4):489-493.ZHANG Baoxi, DIAO Haidan, LI Jiangning.Expressions and clinical significance of lncRNAMALAT1 and miR-200c in ovarian cancer [J]. HebeiMedical Journal,2019,41(4):489-493.
[7] 周连吉,吴标良,杨大伟,等.长链非编码LncRNAMALAT1 在糖尿病肾病患者血清中的表达及临床意义[J].中国老年学杂志,2021,41(1):28-31.ZHOU Lianji, WU Biaoliang, YANG Dawei, et al.Serum level of LncRNA MALAT1 in diabetic kidneydisease patients and its clinical significance [J]. ChineseJournal of Gerontology,2021,41(1):28-31.
[8] 刘天楠.LncRNA MALAT1 在急性心肌梗死中的作用[J].临床与病理杂志, 2020, 40(11):3006-3009.LIU Tiannan. LncRNA MALAT1 in acute myocardialinfarction [J]. International Journal of Pathology andClinical Medicine, 2020, 40(11):3006-3009.
[9] TOMINAGA M,KAWAI M,MINAI K,et al .Association between plasma B-type natriuretic peptideand anaemia in heart failure with or without ischaemicheart disease: a retrospective study[J]. BMJ Open,2019, 9(3): e024194.
[10] 柴春艳,王甜,刘娅萍,等.WWOX 蛋白及Bcl-2 蛋白在支气管肺癌中的表达及临床意义[J].现代检验医学杂志,2017,32(1):61-65.CHAI Chunyan, WANG Tian, LIU Yaping, et al.Expression and clinical significance of WWOX proteinand BCL-2 protein in primary lung cancer [J]. Journalof Modern Laboratory Medicine,2017,32(1):61-65.

相似文献/References:

[1]李德璇,王玉明,杨红玲,等.亲环素A与慢性心力衰竭相关性的研究[J].现代检验医学杂志,2015,30(01):31.[doi:10.3969/j.issn.1671-7414.2015.01.009]
 LI De-xuan,WANG Yu-ming,YANG Hong-ling,et al.Study on the Relationship between Cyclophilin A and Chronic HeartFailure[J].Journal of Modern Laboratory Medicine,2015,30(01):31.[doi:10.3969/j.issn.1671-7414.2015.01.009]
[2]崔 贞,刘 文.慢性心力衰竭患者呋塞米治疗对血清NT-proBNP水平的影响[J].现代检验医学杂志,2019,34(03):144.[doi:10.3969/j.issn.1671-7414.2019.03.037]
 CUI Zhen,LIU Wen.Effects of Furosemide in Patientswith Chronic Heart Failure on Serum NT-proBNP[J].Journal of Modern Laboratory Medicine,2019,34(01):144.[doi:10.3969/j.issn.1671-7414.2019.03.037]
[3]易福凌,陈伟芝,王 禺.慢性心力衰竭患者血清 Mb,cTnI,IL-8 和 hs-CRP 水平表达与心功能分级的相关性研究[J].现代检验医学杂志,2021,36(03):58.[doi:10.3969/j.issn.1671-7414.2021.03.013]
 YI Fu-ling,CHEN Wei-zhi,WANG Yu.Correlation between the Expression of Serum MB, cTnI, IL-8, hs-CRP andCardiac Function in Patients with Chronic Heart Failure[J].Journal of Modern Laboratory Medicine,2021,36(01):58.[doi:10.3969/j.issn.1671-7414.2021.03.013]
[4]王 麟,陈惠新,李小燕.慢性心力衰竭患者血清TRPC1,sFRP5和NT-proBNP水平与心功能及疾病严重程度的临床研究[J].现代检验医学杂志,2021,36(06):6.[doi:10.3969/j.issn.1671-7414.2021.06.002]
 WANG Lin,CHEN Hui-xin,LI Xiao-yan.Study on the Relationship between Serum TRPC1, sFRP5 and NT-proBNP Levels and Cardiac Function and Disease Severity in Predicting with Chronic Heart Failure[J].Journal of Modern Laboratory Medicine,2021,36(01):6.[doi:10.3969/j.issn.1671-7414.2021.06.002]
[5]张卫东,乔明刚.老年心衰并发心律失常患者血清Ang-2,CysC水平及hs-CRP/PAB比值对临床预后评估的价值研究[J].现代检验医学杂志,2021,36(06):128.[doi:10.3969/j.issn.1671-7414.2021.06.027]
 ZHANG Wei-dong,QIAO Ming-gang.Value of Serum Ang-2, Cys C and Hs-CRP/PAB Ratio in the Evaluation of Clinical Prognosis in Elderly Patients with Heart Failure Complicated by Arrhythmia[J].Journal of Modern Laboratory Medicine,2021,36(01):128.[doi:10.3969/j.issn.1671-7414.2021.06.027]
[6]陈 伟,侯湘岭.老年慢性心力衰竭患者血清人类母系表达基因3的水平与预后的相关性研究[J].现代检验医学杂志,2022,37(03):26.[doi:10.3969/j.issn.1671-7414.2022.03.006]
 CHEN Wei,HOU Xiang-ling.Study on the Correlation between Serum Human Maternally Expressed Gene 3 Levels and Prognosis in Elderly Patients with Chronic Heart Failure[J].Journal of Modern Laboratory Medicine,2022,37(01):26.[doi:10.3969/j.issn.1671-7414.2022.03.006]
[7]李岗峰,李刘文,刘 璐.慢性心力衰竭患者血清内源性Apela,NT-proBNP及CysC水平与并发肾功能不全的相关性分析[J].现代检验医学杂志,2022,37(03):167.[doi:10.3969/j.issn.1671-7414.2022.03.035]
 LI Gang-feng,LI Liu-wen,LIU Lu.Correlation Analysis of Serum Endogenous Apela, NT-proBNP, Cys C Levels and Renal Insufficiency in Patients with Chronic Heart Failure[J].Journal of Modern Laboratory Medicine,2022,37(01):167.[doi:10.3969/j.issn.1671-7414.2022.03.035]
[8]杨 婷a,赵茜茜,褚 杰a,等.慢性心力衰竭患者血清MCP-3,NRG-1β 和CF6 水平表达与疾病严重程度的相关性研究[J].现代检验医学杂志,2022,37(05):139.[doi:10.3969/j.issn.1671-7414.2022.05.027]
 YANG Tinga,ZHAO Qian-qian,CHU Jiea,et al.Study on the Correlation between the Expression of Serum MCP-3, NRG-1β and CF6 Levels in Patients with Chronic Heart Failure and the Severity of the Disease[J].Journal of Modern Laboratory Medicine,2022,37(01):139.[doi:10.3969/j.issn.1671-7414.2022.05.027]
[9]徐锦华a,王慧娟,励 国a,等.老年慢性心力衰竭患者血清SCD-1 和sVEGFR-2 表达水平及其与预后的评估价值研究[J].现代检验医学杂志,2023,38(04):191.[doi:10.3969/j.issn.1671-7414.2023.04.035]
 XU Jinhuaa,WANG Huijuan,LI Guoa,et al.Study on the Expression Level of Serum SCD-1 and sVEGFR-2 in Elderly Patients with Chronic Heart Failure and Its Prognostic Value[J].Journal of Modern Laboratory Medicine,2023,38(01):191.[doi:10.3969/j.issn.1671-7414.2023.04.035]
[10]林 艳,但汉良,余 扬,等.慢性心力衰竭患者血清miR-122 和miR-558 水平表达与心功能及心率变异性的相关性研究[J].现代检验医学杂志,2024,39(03):37.[doi:10.3969/j.issn.1671-7414.2024.03.006]
 LIN Yan,DAN Hanliang,YU Yang,et al.Correlation between Serum miR-122 and miR-558 Levels and Cardiac Function and Heart Rate Variability in Patients with Chronic Heart Failure[J].Journal of Modern Laboratory Medicine,2024,39(01):37.[doi:10.3969/j.issn.1671-7414.2024.03.006]

备注/Memo

备注/Memo:
作者简介:王新庄(1980-),男,主治医师,研究方向:急诊常见疾病的临床诊疗,E-mail:838035758@qq.com。
通讯作者:孟建涛(1987-),男,主治医师,研究方向:急诊常见疾病的临床诊疗,E-mail:369145939@qq.com。
更新日期/Last Update: 1900-01-01