[1]冯 芬.结直肠癌组织中USP11 和PPP1CA 表达水平与临床病理的相关性研究[J].现代检验医学杂志,2022,37(04):43-48.[doi:10.3969/j.issn.1671-7414.2022.04.009]
　FENG Fen.Correlation between USP11 and PPP1CA Expression Levels and Clinicopathology in Colorectal Cancer Tissues[J].Journal of Modern Laboratory Medicine,2022,37(04):43-48.[doi:10.3969/j.issn.1671-7414.2022.04.009]
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结直肠癌组织中USP11 和PPP1CA 表达水平与临床病理的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年04期
页码:
43-48
栏目:
论著
出版日期:
2022-07-15

文章信息/Info

Title:
Correlation between USP11 and PPP1CA Expression Levels and Clinicopathology in Colorectal Cancer Tissues
文章编号:
1671-7414（2022）04-043-06
作者:
冯 芬
佛山市第一人民医院胃肠肿瘤内科，广东佛山 528000
Author(s):
FENG Fen
Department of Gastroenterology，the First People’s Hospital of Foshan, Guangdong Foshan 528000,China
关键词:
结直肠癌; 泛素特异性蛋白酶11; 蛋白磷酸酶1 催化亚基α
分类号:
R735.3；R730.43
DOI:
10.3969/j.issn.1671-7414.2022.04.009
文献标志码:
A
摘要:
目的 探讨结直肠癌组织中泛素特异性蛋白酶11(ubiquitin-specific protease 11，USP11)、蛋白磷酸酶1 催化亚基α(protein phosphatase 1 catalytic subunit α，PPP1CA) 表达水平与临床病理的相关性。方法 收集佛山市第一人民医院2015 年1 月～ 2016 年4 月87 例结直肠癌患者外科手术切除的癌组织和癌旁组织标本及临床资料。采用实时荧光定量PCR 检测USP11 mRNA 和PPP1CA mRNA 表达水平，免疫组织化学检测USP11 和PPP1CA 蛋白表达水平。采用Spearman 相关性分析结直肠癌组织中USP11 mRNA 与PPP1CA mRNA 表达的相关性，并分析二者与结直肠癌临床病理特征的关系。术后随访5 年，统计总生存率，K M 法绘制USP11 和PPP1CA 蛋白表达阳性与阴性结直肠癌患者生存曲线。采用多因素COX 回归分析结直肠癌患者预后不良影响因素。结果 癌组织中USP11 mRNA[3.49(3.45,3.52)] 和PPP1CAmRNA 表达水平[1.13(1.11,1.15)] 高于癌旁组织[1.02(0.99,1.06)，0.35(0.34,0.36)], 差异均有统计学意义（ Z= -11.397，-11.423，均P ＜ 0.001）。结直肠癌组织中USP11 mRNA 与PPP1CA mRNA 表达呈正相关(rs =0.658，P ＜ 0.001)。USP11 和PPP1CA 表达与结直肠癌TNM 分期和淋巴结转移有关(χ2=5.386 ～ 6.471，均P ＜ 0.05)，与性别、年龄、组织学分型和分化程度无关(χ2=0.039 ～ 1.130，均P ＞ 0.05)。中位随访34 个月，术后5 年总生存率为64.37%，USP11和PPP1CA 蛋白表达阳性组术后5 年总生存率为55.17% 和57.41%，低于USP11 和PPP1CA 蛋白表达阴性组（82.76%，75.76%），差异有统计学意义（Log-rankχ2=8.900，9.081，均P＜ 0.01)。TNM分期Ⅲ～Ⅳ期(HR=1.896，95%CI 1.359 ～ 2.749)、淋巴结转移(HR=1.818，95%CI 1.391 ～ 2.698)、USP11 阳性(HR=2.404，95%CI 1.791 ～ 3.754)、PPP1CA 阳性(HR=2.556，95%CI 1.868 ～ 3.852) 为结直肠癌患者预后不良独立风险因素(P=0.009，0.013，0.019，0.010)。结论 结直肠癌中USP11 和PPP1CA 高表达，与TNM 分期和淋巴结转移有关，二者可能共同影响患者预后。
Abstract:
Objective To investigate the correlation between the expression levels of ubiquitin-specific protease 11 (USP11), protein phosphatase 1 catalytic subunit alpha (PPP1CA) and clinicopathology in colorectal cancer tissues. Methods The specimens and clinical data of cancer tissues and adjacent tissues surgically removed from 87 patients with colorectal cancer in the First People’s Hospital of Foshan from January 2015 to April 2016 were collected. The expression levels of USP11 mRNA and PPP1CA mRNA were detected by real-time fluorescence quantitative PCR, and the expression levels of USP11 and PPP1CA protein were detected by immunohistochemistry. Spearman correlation was used to analyze the correlation between the expression of USP11 mRNA and PPP1CA mRNA in colorectal cancer tissues, and to analyze the relationship between the two and the clinicopathological characteristics of colorectal cancer.After 5 years of follow-up, the overall survival rate was calculated, and the K-M method was used to draw the survival curve of USP11, PPP1CA protein expression positive and negative colorectal cancer patients. Multivariate COX regression was used to analyze the factors affecting the prognosis of patients with colorectal cancer. Results The expression levels of USP11 mRNA［3.49(3.45,3.52)］, PPP1CA mRNA［1.13(1.11,1.15)］ were higher in cancer tissues than in paracancerous tissues［1.02(0.99,1.06)，0.35(0.34,0.36) ］，the differences were statistically significant (Z =-11.397,-11.423, all P ＜ 0.001).USP11 and PPP1CA expression was associated with TNM stage and lymph node metastasis in colorectal cancer(χ2=5.386 ～ 6.471, all P ＜ 0.05), independent of gender, age, histological staging and degree of differentiation (χ2=0.039 ～ 1.130, all P ＞ 0.05). There was a positive correlation between the expression of USP11 mRNA and PPP1CA mRNA in colorectal cancer tissues (rs =0.658, P ＜ 0.001).At a median follow-up of 34 months,the overall survival rate at 5 years after surgery was 64.37%, 55.17% and 57.41% in the USP11, PPP1CA protein expression positive group were lower than 82.76% and 75.76% in the USP11, PPP1CA protein expression negative group ，the differences were statistically significant(Log-rank χ2=8.900，9.081, all P =0.003).TNM stage Ⅲ～Ⅳ (HR=1.896, 95%CI 1.359 ～ 2.749), lymph node metastasis (HR=1.818, 95%CI 1.391 ～ 2.698), USP11 positive (HR=2.404, 95%CI 1.791 ～ 3.754), PPP1CA positive (HR=2.556, 95%CI 1.868 ～ 3.852) were independent risk factors for poor prognosis in patients with colorectal cancer (P=0.009, 0.013, 0.019, 0.010). Conclusion The high expression of USP11 and PPP1CA in colorectal cancer was related to TNM staging and lymph node metastasis, and both may affect the prognosis of patients.

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备注/Memo

备注/Memo:
基金项目：2019 年佛山市自筹经费类科技计划项目（1920001001013）。
作者简介：冯芬（1983-），男，硕士，副主任医师，研究方向：胃肠道肿瘤，E-mail:ff198304@163.com。
更新日期/Last Update: 1900-01-01