[1]柴亮听,张 卉,冯 艳,等.急性心肌梗死患者血清miR-423-5P，Bcl-2 和LC3-II 水平表达与预后相关性研究[J].现代检验医学杂志,2023,38(01):117-120+134.[doi:10.3969/j.issn.1671-7414.2023.01.022]
　CHAI Liang-ting,ZHANG Hui,FENG Yan,et al.Correlation between Serum of miR-423-5p, Bcl-2 and LC3-II and Prognosis in Patients with Acute Myocardial Infarction[J].Journal of Modern Laboratory Medicine,2023,38(01):117-120+134.[doi:10.3969/j.issn.1671-7414.2023.01.022]
点击复制

急性心肌梗死患者血清miR-423-5P，Bcl-2 和LC3-II 水平表达与预后相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年01期
页码:
117-120+134
栏目:
论著
出版日期:
2023-01-15

文章信息/Info

Title:
Correlation between Serum of miR-423-5p, Bcl-2 and LC3-II and Prognosis in Patients with Acute Myocardial Infarction
文章编号:
1671-7414（2023）01-117-05
作者:
柴亮听1; 张 卉2; 冯 艳1; 索 瑞1;  左惠芬1; 刘冬青1
（1．邯郸市第一医院检验科，河北邯郸 056000；2．邯郸市中心医院生殖医学科，河北邯郸 056000）
Author(s):
CHAI Liang-ting1; ZHANG Hui2; FENG Yan1;  SUO Rui1; ZUO Hui-fen1;  LIU Dong-qing1
（1. Department of Clinical Laboratory, the First Hospital of Handan City, Hebei Handan 056000, China; 2 .Department of Reproductive Medicine, Handan Central Hospital, Hebei Handan 056000, China）
关键词:
急性心肌梗死; 微小核糖核酸-423-5P; B 细胞淋巴瘤因子-2; 不良心血管事件
分类号:
R542.22；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.01.022
文献标志码:
A
摘要:
目的 探讨血清miR-423-5P，Bcl-2 和LC3-II 对急性心肌梗死(acute myocardial infarction，AMI)患者预后的影响。方法 筛选2017 年3 月～ 2018 年10 月收治于邯郸市第一医院心内科的122 例AMI患者为研究对象，根据随访期间（一年）是否发生主要不良心血管事件（major adverse cardiovascular events，MACE）分为MACE组（n=43）和非MACE组（n=79），同期体检健康志愿者（n=50）作为对照组，分别采用聚合酶链式反应（polymerase chain reaction，PCR）和酶联免疫吸附测定（enzyme linked immunosorbent assay，ELISA）检测患者血清miR-423-5P，Bcl-2 和LC3-II 表达水平。比较两组患者血清miR-423-5P，Bcl-2 和LC3-II 的表达差异，并用受试者工作特征(receiver operator characteristic, ROC) 曲线图分析对急性心肌梗死患者预后的价值。结果 对照组miR-423-5P（0.91±0.12），Bcl-2（1.13±0.21ng/ml）和LC3-II（0.73±0.16 ng/ml）均低于MACE组（1.42±0.22，4.25±0.34 ng/ml，1.25±0.31g/ml）和非MACE组（1.13±0.11，2.01±0.28ng/ml，1.21±0.06 ng/ml），差异均具有统计学意义（F=151.155，1 880.468，115.625，均P ＜ 0.05）；MACE 组血清mIR-423-5P 和Bcl-2 表达均高于非MACE 组，差异具有统计学意义（t=9.716, 39.093，均P ＜ 0.05），两组间血清LC3-II 的表达差异无统计学意义（t=1.113, P>0.05）。ROC 曲线显示，miR-423-5P，Bcl-2 预测急性心肌梗死患者预后的曲线下面积分别为0.813 和0.776，截断值分别为1.213 和4.012 ng/ml，灵敏度分别为0.721 和0.702，特异度分别为0.734和0.737；二者联合检测时，曲线下面积、灵敏度和特异度分别为0.852, 0.832，0.827。结论 血清mIR-423-5P，Bcl-2水平与急性心肌梗死患者预后密切相关，有可能成为判断预后的标志物，且两者联合应用的效果优于单独应用。
Abstract:
Objective Aim to investigate the effects of serum miR-423-5P，Bcl-2 and LC3-II on the prognosis of patients with acute myocardial infarction（AMI）. Methods 122 patients with AMI treated in the Department of Cardiology of the First Hospital of Handan City from March 2017 to October 2018 were selected as the research objects. According to whether major adverse cardiovascular events (MACE) occurred during the follow-up period (1 year), they were divided into mace group (n=43 ) and non mace group (n=79 ). Healthy volunteers (n=50 ) in the same period were used as the control group. The expression levels of serum miR-423-5p, Bcl-2 and LC3-II were detected by PCR and ELISA. The expression differences of serum miR-423-5p, Bcl-2 and LC3-II between the two groups were compared, and the value of ROC curve in the prognosis of patients with acute myocardial infarction was analyzed. Results MiR-423-5p (0.91 ± 0.12), Bcl-2 (1.13 ± 0.21ng/ml) and LC3-II (0.73 ± 0.16 ng/ml) in the control group were lower than those in MACE group (1.42 ± 0.22, 4.25 ± 0.34 ng/ml, 1.25 ± 0.31 ng/ml) and non MACE group (1.13 ± 0.11, 2.01 ± 0.28 ng/ml, 1.21 ± 0.06 ng/ml), and the differences were statistically significant (F=151.155，1 880.468，115.625，all P < 0.05). The expressions of serum miR-423-5p and Bcl-2 in the MACE group were higher than those in the non-MACE group, with statistically significant differences (t=9.716, 39.093, all P< 0.05), while the differences in LC3-II expression between the two groups were not statistically significant (t=1.113, P> 0.05). The area under the curve of miR-423-5p and Bcl-2 in predicting the prognosis of patients with AMI were 0.813 and 0.776, cut-off value were 1.213 and 4.012 ng/ml, sensitivity were 0.721 and 0.702, specificity were 0.734 and 0.737, respectively. When the two were combined, the area under the curve, the sensitivity and the specificity were 0.852, 0.832, 0.827. Conclusion Serum miR-423-5p and Bcl-2 levels are closely related to the prognosis of patients with acute myocardial infarction, and may be used as prognostic markers, and the combined application of the two is better than the single application.

参考文献/References:

[1] 马丽媛, 吴亚哲, 王文, 等．《中国心血管病报告2017》要点解读[J]. 中国心血管杂志, 2018, 23 (1):3-6.
MA Liyuan, WU Yazhe, WANG Wen, et al . Interpretation of the report on cardiovascular diseases in China（2017）[J]. Chinese Journal of Cardiovascular Medicine, 2018,23 (1): 3-6.
[2] 郭国锋, 宋代富, 高嘉鑫, 等. 急性心肌梗死后心脏破裂的发生机制[J]. 中华老年心脑血管病杂志,2019, 21(11):1214-1216.
GUO Guofeng, SONG Daifu, GAO Jiaxin, et al.Mechanism of cardiac rupture after acute myocardial infarction [J]. Chinese Journal of Geriatric Heart Brain and Vessel Diseases,2019, 21 (11): 1214-1216.
[3] 高青豹, 戴喜明, 胡清甫, 等. 急性冠状动脉综合征的老年患者预后与心率变异性关系[J]. 中华老年心脑血管病杂志,2019, 21(12),1293-1296.
GAO Qingbao, DAI Ximing, HU Qingfu, et al.Relationship between outcome and heart rate variability in elderly acure coronary syndrome patients[J]. Chinese Journal of Geriatric Heart Brain and Vessel Diseases,2019,21 (12): 1293-1296.
[4] 宋倩, 张雯．急性心肌梗死患者血浆PCSK9 水平对临床不良事件发生的预测价值分析[J]. 现代检验医学杂志, 2020, 35(3): 72-77.
SONG Qian, ZHANG Wen. Analysis of the predictive value of plasma PCSK9 level on clinical adverse events in patients with acute myocardial infarction [J].Journal of Modern Laboratory Medicine, 2020, 35(3):72-77.
[5] LI G Q, CHEN M. Cardioprotective effect of erythropoietin in rats with acute myocardial infarction through JNK pathway[J]. European Review for Medical and Pharmacological Sciences, 2019, 23(3 Suppl): 153-160.
[6] LIN Bin, FENG Deguang, XU Jing. Cardioprotective effects of microRNA-18a on acute myocardial infarction by promoting cardiomyocyte autophagy and suppressing cellular senescence via brain derived neurotrophic factor[J]. Cell Biosci, 2019, 9:38.
[7] 冯艳, 李延民．缬沙坦联合瑞舒伐他汀治疗急性心肌梗死的疗效及对患者血清相关指标的影响[J]. 中国医院用药评价与分析, 2019, 19(5):552-554, 559.
FENG Yan, LI Yanmin. Efficacy of valsartan combined with rosuvastatin in treatment of acute myocardial infarction and its effects on serum related indicators [J].Evaluation and Analysis of Drug-Use in Hospitals of China, 2019,19 (5): 552-554, 559.
[8] 周顺利. 心肌梗死溶栓危险指数对急性心肌梗死介入治疗远期预后的评估价值[J]. 护理实践与研究,2019, 16(23):6-8.
ZHOU Shunli. Evaluation value of thrombolytic risk index of myocardial infarction for long-term prognosis of interventional therapy for acute myocardial infarction[J]. Nursing Practice and Research, 2019,16 (23): 6-8.
[9] RUPAIMOOLE R, SLACK F J. MicroRNA therapeutics:towards a new era for the management of cancer and other diseases[J]. Nature Reviews Drug Discovery,2017, 16(3): 203-221.
[10] SHEN Yahui, LU Huiyu, CHEN Rong, et al.MicroRNA-29c affects zebrafish cardiac development via targeting Wnt4[J]. Molecular Medicine Reports,2020, 22(6): 4675-4684.
[11] RIZZACASA B, MORINI E, MANGO R, et al. MiR-423 is differentially expressed in patients with stable and unstable coronary artery disease: A pilot study[J].PLoS One, 2019, 14(5): e0216363.
[12] WANG Weisong, GAO Junjie, WANG Fangli. MiR-663a/MiR-423-5p are involved in the pathogenesis of lupus nephritis via modulating the activation of NF-κB by targeting TNIP2[J]. American Journal of Translational Research, 2017, 9(8): 3796-3803.
[13] LALEM T, DEVAUX Y. Circulating microRNAs to predict heart failure after acute myocardial infarction in women[J]. Clinical Biochemistry, 2019, 70(8): 1-7.
[14] LIU Langsha, CHEN Yubin, SHU Jie, et al.Identification of microRNAs enriched in exosomes in human pericardial fluid of patients with atrial fibrillation based on bioinformatic analysis[J]. Journal of Thoracic Disease, 2020, 12(10): 5617-5627.
[15] WANG Weisong, GAO Junjie, WANG Fangli. MiR-663a/MiR-423-5p are involved in the pathogenesis of lupus nephritis via modulating the activation of NF-kB by targeting TNIP2[J]. American Journal of Translational Research, 2017, 9(8): 3796-3803.
[16] 周奋, 郑霞, 毛海燕, 等. 急性心肌梗死大鼠中微小RNA-221 的表达及相关机制[J]. 中华实验外科杂志,2019, 36(6)：1080-1082．
ZHOU Fen, ZHENG Xia, MAO Haiyan, et al.Expression of microRNA-221 and its related mechanisms in rats with acute myocardial infarction [J].Chinese Journal of Experimental Surgery, 2019,36 (6):1080-1082.
[17] LIN Haili, LIN Tiangqi, LIN Jiangui, et al. Inhibition of miR-423-5p suppressed prostate cancer through targeting GRIM-19[J]. Gene, 2019, 688: 93-97.
[18] 柴松波, 王振涛, 张淑娟, 等. 心衰康抑制慢性心衰大鼠心肌自噬及MAPK/ERK1/2 信号通路[J]. 中国病理生理杂志, 2019, 35(6):981-987．
CHAI Songbo, WANG Zhentao, ZHANG Shujuan, et al. Xinshuaikang inhibits myocardial autophagy and MAPK/ERK1/2 signaling pathway in rats with chronic heart failure [J]. Chinese Journal of Pathophysiology,2019,35 (6): 981-987.
[19] SIREGAR T A P, PROMBUTARA P, KANJANASIRIRAT P, et al. The autophagy-resistant Mycobacterium tuberculosis Beijing strain upregulates KatG to evade starvation-induced autophagic restriction[J].Pathog Dis, 2022, 80(1): ftac004.
[20] MOKHTARI B, BADALZADEH R. The potentials of distinct functions of autophagy to be targeted for attenuation of myocardial ischemia/reperfusion injury in preclinical studies: an up-to-date review [J]. Journal of Physiology and Biochemistry, 2021, 77(3): 377-404.
[21] TANGCHIRAKHAPHAN S, INNAJAK S,NILWARANGKOON S, et al. Mechanism of apoptosis induction associated with ERK1/2 upregulation via goniothalamin in melanoma cells [J]. Experimental and Therapeutic Medicine, 2018, 15(3): 3052-3058.
[22] 王鹏贺, 周思维, 张莎, 等. 自噬在急性心肌梗死发病中作用的研究进展[J]. 现代生物医学进展, 2018,18(4):783-786.
WANG Penghe, ZHOU Siwei, ZHANG Sha, et al.Research progress of the role of autophagy in the pathogenesis and progression of acute myocardial infarction [J]. Progress in Modern Biomedicine,2018,18 (4): 783-786.

相似文献/References:

[1]宋　倩a,张　雯b.急性心肌梗死患者血浆PCSK9 水平对临床不良事件发生的预测价值分析[J].现代检验医学杂志,2020,35(03):72.[doi:10.3969/j.issn.1671-7414.2020.03.018]
　SONG Qiana,ZHANG Wenb.Analysis of the Predictive Value of Plasma PCSK9 Level on Clinical Adverse Events in Patients with Acute Myocardial Infarction[J].Journal of Modern Laboratory Medicine,2020,35(01):72.[doi:10.3969/j.issn.1671-7414.2020.03.018]
[2]达晶,张超,吴红葵,等.急性心肌梗死患者血浆miRNA-423-5p 与整合素连接激酶水平表达对评价近期临床预后的价值[J].现代检验医学杂志,2020,35(05):33.[doi:10.3969/j.issn.1671-7414.2020.05.009]
　DA Jing,ZHANG Chao,WU Hong-kui,et al.Expression of Plasma miRNA-423-5p and Integrin-linked Kinase Levels in Patients with Acute Myocardial Infarction for Evaluating Recent Clinical Outcomes[J].Journal of Modern Laboratory Medicine,2020,35(01):33.[doi:10.3969/j.issn.1671-7414.2020.05.009]
[3]刘　云,孙静芳,马　萍.外周血血细胞参数在急性主动脉夹层与急性心肌梗死鉴别中的价值[J].现代检验医学杂志,2021,36(01):132.[doi:10.3969/j.issn.1671-7414.2021.01.033]
　LIU Yun,SUN Jing-fang,MA Ping.Value of Peripheral Blood Cell Parameters in Differentiating Acute Aortic Dissection from Acutemyocardial Infarction[J].Journal of Modern Laboratory Medicine,2021,36(01):132.[doi:10.3969/j.issn.1671-7414.2021.01.033]
[4]李　娟,张芬红,徐卓婧,等.老年急性 ST 段抬高型心肌梗死患者血浆 miR-499-5p 表达水平对并发急性心力衰竭的预测价值[J].现代检验医学杂志,2021,36(03):48.[doi:10.3969/j.issn.1671-7414.2021.03.011]
　LI Juana,ZHANG Fen-honga,XU Zhuo-jinga,et al.Predictive Value of Plasma miR-499-5p for Acute Heart Failure afterAcute ST-Elevated Myocardial Infarction in Elderly Patients[J].Journal of Modern Laboratory Medicine,2021,36(01):48.[doi:10.3969/j.issn.1671-7414.2021.03.011]
[5]杜春娟,李　丹,王　岑,等.急性心肌梗死并发阿斯综合征患者血清和肽素与 Adropin 水平联合检测的早期诊断价值研究[J].现代检验医学杂志,2021,36(03):97.[doi:10.3969/j.issn.1671-7414.2021.03.022]
　DU Chun-juan,WANG Cen,HUYAN-Li,et al.Study on the Early Diagnostic Value of Serum Copeptin and Adropin Levels in Patients with Acute Myocardial Infarction Complicated with Adams-stokes Syndrome[J].Journal of Modern Laboratory Medicine,2021,36(01):97.[doi:10.3969/j.issn.1671-7414.2021.03.022]
[6]刘 蕾a,赵 鹏b,张 军a,等.急性心肌梗死患者血浆外泌体miR-21-3p/PTEN/AKT 表达水平及诊断价值研究[J].现代检验医学杂志,2022,37(05):118.[doi:10.3969/j.issn.1671-7414.2022.05.023]
　LIU Leia,ZHAO Pengb,ZHANG Juna,et al.Serum Exosomal miR-21-3p/PTEN/AKT Expression and Diagnostic Value in Patients with Acute Myocardial Infarction[J].Journal of Modern Laboratory Medicine,2022,37(01):118.[doi:10.3969/j.issn.1671-7414.2022.05.023]

备注/Memo

备注/Memo:
基金项目：邯郸市科技局项目（No.1623208064-5）。
作者简介：柴亮听（1981-），男，本科，副主任技师，从事临床检验的研究，E-mail:cliang637@163.com。
更新日期/Last Update: 2023-01-15