[1]夏慧娟,付小梅,陈秋月.肺炎支原体感染诱发哮喘患儿血清中miR-424-5p 和CX3CL1 表达水平及与预后预测价值研究[J].现代检验医学杂志,2023,38(04):100-104+168/.[doi:10.3969/j.issn.1671-7414.2023.04.018]
　XIA Huijuan,FU Xiaomei,CHEN Qiuyue.Study on the Expression of miR-424-5p and CX3CL1 in Serum and Prognosis Predictive Value of Children with Asthma Induced by Mycoplasma Pneumoniae Infection[J].Journal of Modern Laboratory Medicine,2023,38(04):100-104+168/.[doi:10.3969/j.issn.1671-7414.2023.04.018]
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肺炎支原体感染诱发哮喘患儿血清中miR-424-5p 和CX3CL1 表达水平及与预后预测价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年04期
页码:
100-104+168/
栏目:
论著
出版日期:
2023-07-15

文章信息/Info

Title:
Study on the Expression of miR-424-5p and CX3CL1 in Serum and Prognosis Predictive Value of Children with Asthma Induced by Mycoplasma Pneumoniae Infection
文章编号:
1671-7414（2023）04-100-06
作者:
夏慧娟; 付小梅; 陈秋月
（内江市第一人民医院儿科，四川内江 641000）
Author(s):
XIA Huijuan;  FU Xiaomei;  CHEN Qiuyue
（Department of Pediatrics, the First People’s Hospital of Neijiang， Sichuan Neijiang 641000，China）
关键词:
肺炎支原体感染; 哮喘; 微小核糖核酸-424-5p; CX3CL1
分类号:
R375.2；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.04.018
文献标志码:
A
摘要:
目的　检测肺炎支原体（Mycoplasma pneumoniae，MP）感染诱发哮喘患儿血清中微小核糖核酸（micro RNA,miR）-424-5p 和CX3CL1 表达水平及其与患儿预后的关系。方法　选取2020 年3 月～ 2021 年5 月在内江市第一人民医院进行治疗的MP 感染患儿128 例作为研究对象，根据是否诱发哮喘分为哮喘组（n=82）和MP 组（n=46），另选取50 例健康儿童作为对照组。分别比较哮喘组、MP 组和对照组血清miR-424-5p，CX3CL1，IgE，IL-6，TNF-α 水平和肺功能指标；根据哮喘患儿预后情况分为预后良好组（n=50）和预后不良组（n=32），分析哮喘患儿预后情况与临床病理特征的关系；采用Kaplan-Meier 法分析MP 诱发哮喘患儿血清中miR-424-5p 和CX3CL1 表达与患儿预后的关系；采用多因素Logisitic 回归分析MP诱发哮喘患儿预后的影响因素。结果　对照组、MP组和哮喘组血清miR-424-5p（0.95±0.26，1.12±0.35，1.34±0.39），CX3CL1（0.47±0.08，0.62±0.17，0.91±0.22 ng/ml），Ig E（22.64±5.74，41.26±8.91，52.31±10.26 IU/ml），IL-6（5.26±0.90，8.24±1.07，9.14±1.18 ng/L） 和TNF-α（55.27±10.54，88.56±18.67,105.26±26.48 pg/ml） 水平依次升高， 差异具有统计学意义（F=20.287，103.274，174.470， 205.317，87.349， 均P<0.05）。对照组、MP 组和哮喘组肺功能指标VC（80.24±19.35，62.35±15.48 ，51.26±12.38 ml），FVC（78.35%±18.29%，60.21%±16.34 %，48.36%±11.73 %）和FEV1（63.31%±17.26 %，44.26%±10.31 %，38.69%±9.58 %）依次降低，差异具有统计学意义（F=54.943，61.821，63.006，均P<0.05）。哮喘患儿预后良好组血清Ig E，IL-6，TNF-α，miR-424-5p 和CX3CL1 水平均明显低于预后不良组，差异具有统计学意义（t=3.825，6.137，3.086，4.003，6.044，均P<0.05）。Kaplan-Meier 法分析显示，血清miR-424-5p 低表达组和CX3CL1 低表达组患儿预后良好率高于高表达组（χ2=4.377，4.172，均P ＜ 0.05）。多因素Logistic 回归分析结果显示，Ig E，IL-6，TNF-α，miR-424-5p 和CX3CL1水平为MP 诱发哮喘患儿预后的影响因素（P<0.05）。结论　MP 诱发哮喘患儿血清miR-424-5p 和CX3CL1 水平上调，且二者水平变化与患儿预后情况密切相关。
Abstract:
Objective　To detect the expression levels of miR-424-5p and CX3CL1 in serum of children with asthma induced by Mycoplasma pneumoniae (MP) infection, and analyze their correlation with the prognosis of children. Methods　A total of 128 children with MP infection who were treated in the First People’s Hospital of Neijiang, from March 2020 to May 2021 were selected. According to whether asthma was induced, they were divided into asthma group (n=82) and MP group (n=46), another 50 healthy children were selected as the control group. The serum levels of miR-424-5p, CX3CL1, IgE, IL-6, TNF-α and lung function indexes in asthma group, MP group and control group were compared. According to the prognosis of children with asthma, they were divided into good prognosis group(n=50) and poor prognosis group(n=32). Kaplan-Meier method was applied to analyze the relationship between the expression of miR-424-5p and CX3CL1 in serum and the prognosis of children with MP induced asthma, and multivariate logistic regression was applied to analyze the factors influencing the prognosis of children with MP induced asthma. Results　The levels of serum miR-424-5p（0.95±0.26，1.12±0.35，1.34±0.39）, CX3CL1（0.47±0.08 ，0.62±0.17，0.91±0.22 ng/ml）, IgE（22.64±5.74，41.26±8.91，52.31±10.26 IU/ml）, IL-6（5.26±0.90，8.24±1.07， 9.14±1.18 ng/L） and TNF-α（55.27±10.54，88.56±18.67, 105.26±26.48 pg/ml） in the control group, MP group and asthma group increased in turn, and the differences were statistically significant（F=20.287，103.274，174.470， 205.317， 87.349，all P<0.05）. The pulmonary function indexes VC（80.24±19.35，62.35±15.48 ，51.26±12.38 ml），FVC （78.35%±18.29 %，60.21%±16.34 %，48.36%±11.73 %）and FEV1（63.31%±17.26 %，44.26%±10.31 %， 38.69%±9.58 %）in the control group, MP group and asthma group decreased in turn, and the differences were statistically significant (F=54.943，61.821，63.006，all P<0.05). The levels of serum IgE，IL-6, TNF-α, miR-424-5p and CX3CL1 in children with asthma with good prognosis were greatly lower than those in children with poor prognosis, and the differences were statistically significant (t=3.825，6.137，3.086，4.003，6.044，all P<0.05). Kaplan-Meier analysis showed that the good prognosis rate of children in the low expression group of serum miR-424-5p was greatly higher than that in the high expression group(χ2=4.377, P ＜ 0.05), and the good prognosis rate of children with low expression of CX3CL1 was greatly higher than that of children with high expression of CX3CL1(χ2=4.172, P ＜ 0.05). Multivariate logistic regression analysis showed that the levels of IgE, IL-6, TNF-α, miR-424-5p and CX3CL1 were the prognostic factors of MP induced asthma in children (P<0.05). Conclusion　The levels of miR-424-5p and CX3CL1 in serum of children with asthma induced by MP were up-regulated, and their changes were closely related to the prognosis of children.

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备注/Memo

备注/Memo:
作者简介：夏慧娟（1987-），女，本科，主治医师，研究方向：新生儿呼吸窘迫综合征，E-mail：ntutmx@163.com。
更新日期/Last Update: 2023-07-15