[1]王桂瑜,欧阳葵,苏小田,等.缺血性脑卒中患者血清LncRNA MEG8 表达水平及临床价值研究[J].现代检验医学杂志,2023,38(04):105-109.[doi:10.3969/j.issn.1671-7414.2023.04.019]
　WANG Guiyu,OUYANG Kui,SU Xiaotian,et al.Expression Level of Serum LncRNA MEG8 and Its Clinical Significance in Patients with Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2023,38(04):105-109.[doi:10.3969/j.issn.1671-7414.2023.04.019]
点击复制

缺血性脑卒中患者血清LncRNA MEG8 表达水平及临床价值研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年04期
页码:
105-109
栏目:
论著
出版日期:
2023-07-15

文章信息/Info

Title:
Expression Level of Serum LncRNA MEG8 and Its Clinical Significance in Patients with Ischemic Stroke
文章编号:
1671-7414（2023）04-105-05
作者:
王桂瑜; 欧阳葵; 苏小田; 胡琼文
（海南省老年病医院老年科，海口 571100）
Author(s):
WANG Guiyu; OUYANG Kui; SU Xiaotian; HU Qiongwen
（Department of Gerontology, Hainan Provincial Gerontology Hospital, Haikou 571100，China）
关键词:
缺血性脑卒中; 长链非编码RNA 母系表达基因8; 病情严重程度
分类号:
R743.33；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.04.019
文献标志码:
A
摘要:
目的　探讨缺血性脑卒中患者血清中长链非编码RNA（long-chain non-coding RNA，LncRNA）母系表达基因8（maternal expressed gene 8，MEG8）的表达及其临床价值。方法　选取2021 年1 月～ 2022 年4 月在海南省老年病医院接受治疗的80 例缺血性脑卒中患者为观察组，及同期85 例健康体检志愿者为对照组。参照美国国立卫生研究院卒中量表（NIHSS）评分将患者分为轻度患者组（n=25，NIHSS<5 分）、中度患者组（n=29，5 ≤ NIHSS ≤ 20 分）和重度患者组（n=26，NIHSS>20 分）。采用实时荧光定量PCR（qRT-PCR）法对受试者血清中LncRNA MEG8 的水平进行测定并对不同程度组患者血清LncRNA MEG8 水平进行比较。患者血清中LncRNA MEG8 与C- 反应蛋白（CRP）水平的相关性进行Pearson 分析；Spearman 法对患者LncRNA MEG8 与NIHSS 评分的关系进行分析；Logistic 分析影响缺血性脑卒中发生的因素；受试者工作特征（ROC）曲线分析LncRNA MEG8 水平诊断缺血性脑卒中的价值。结果　与对照组相比，观察组血清中LncRNA MEG8 显著升高（1.55±0.31 vs 1.01±0.15），差异有统计学意义（t=14.373，P=0.000）；与轻度患者组（1.26±0.18）相比，中度与重度患者组LncRNA MEG8 表达水平随着病情的加重依次显著升高（1.54±0.27，1.84±0.48），差异有统计学意义（F=19.262，P=0.000）；缺血性脑卒中患者血清中LncRNA MEG8与CRP 水平、NIHSS 评分呈正相关（r=0.412，0.488；P=0.000 5，0.000 3）；高血压（OR=3.314，95%CI 1.045 ～ 10.513）、高脂血症（OR=4.340，95%CI 1.008 ～ 18.692）、糖尿病（OR=7.891，95%CI 1.024 ～ 60.828），高LncRNA MEG8（OR=7.021，95%CI 4.316 ～ 37.440）和高CRP（OR=2.415，95%CI 1.207 ～ 4.833）是发生缺血性脑卒中的危险因素（P=0.042，0.048，0.047，0.022，0.013）；LncRNA MEG8 水平诊断缺血性脑卒中的ROC 曲线下面积为0.923（95%CI: 0.880 ～ 0.966），截断值为1.14，敏感度和特异度分别为81.3%，94.1%。结论　缺血性脑卒中患者LncRNA MEG8 表达升高，与患者病情严重程度相关，可较好地诊断缺血性脑卒中。
Abstract:
Objective　To investigate the expression of maternal expression gene 8 (MEG8) of long-chain non-coding RNA (LncRNA) in the serum of patients with ischemic stroke and its clinical significance. Methods　80 patients with ischemic stroke who were treated in Geriatric Hospital of Hainan from January 2021 to April 2022 were regarded as the observation group, and 85 healthy volunteers in the same period were included as the control group. The severity of all patients was evaluated according to the National Institutes of Health Stroke Scale (NIHSS), which was grouped into mild patients (n=25，NIHSS<5 score)， moderate patients (n=29，5 ≤ NIHSS ≤ 20 score) and severe patients (n=26，NIHSS>20 score). The relative expression level of LncRNA MEG8 in serum of all subjects was detected by real-time fluorescent quantitative PCR (qRT-PCR), and compared between patients in different degrees group. The correlation between LncRNA MEG8 and C-reactive protein (CRP) levels in serum of patients with ischemic stroke was analyzed by Pearson. Spearman method was used to analyze the correlation between LncRNA MEG8 and NIHSS score in patients with ischemic stroke. Logistic regression was performed to analyze the factors influencing the occurrence of ischemic stroke, the diagnostic value of the expression level of LncRNA MEG8 in ischemic stroke was analyzed by ROC curve. Results　The expression level of serum LncRNA MEG8 in control group and observation group were 1.05±0.15 and 1.55±0.31, respecitvely. Compared with the control group, the level of LncRNA MEG8 in serum of the observation group was obviously higher, the difference was statistically significant (t=14.373, P=0.000). Compared with mild patients group (1.26±0.18), the level of LncRNA MEG8 expression in moderate and severe patients group increased significantly with the aggravation of the disease (1.54 ± 0.27, 1.84 ± 0.48), the difference was statistically significant (F=19.262，P=0.000). The serum LncRNA MEG8 in patients with ischemic stroke was positively correlated with CRP level and NIHSS score (r=0.412, 0.488, P=0.000 5, 0.000 3).Logistic regression analysis showed that hypertension (OR=7.891, 95%CI：1.024 ～ 60.828), hyperlipidemia (OR=4.340, 95%CI：1.008 ～ 18.692), diabetes (OR=7.891, 95%CI：1.024 ～ 60.828), LncRNA MEG8 (OR=7.021, 95%CI：4.316 ～ 37.440), CRP (OR=2.415, 95%CI：1.207 ～ 4.833) were risk factors for ischemic stroke (P=0.042, 0.048, 0.047, 0.022, 0.013). The area under the ROC curve (AUC) of ischemic stroke diagnosed by the level of LncRNA MEG8 was 0.923 (95%CI：0.880 ～ 0.966), and the cut-off value was 1.14, the sensitivity and specificity were 81.3% and 94.1%, respectively. Conclusion　The expression of LncRNA MEG8 was increased in patients with ischemic stroke, which is related to the severity of the patient's condition and can better diagnose ischemic stroke.

参考文献/References:

[1] JAYARAJ R L, AZIMULLAH S, BEIRAM R, et al. Neuroinflammation: friend and foe for ischemic stroke[J]. Journal of Neuroinflammation, 2019, 16(1): 142.
[2] 杜龙庭, 勿坡巫且, 许春梅, 等. 缺血性脑卒中患者血清miR-155 和PDCD4 mRNA 表达水平与颈动脉支架成形术后再狭窄的相关性研究[J]. 现代检验医学杂志, 2022, 37(2): 105-109, 172. DU Longting, WUPO Wuqie, XU Chunmei, et al. Correlation between the expression of miR-155, PDCD4 mRNA and restenosis after carotid artery stenting in patients with cerebral ischemic stroke [J]. Journal of Modern Laboratory Medicine, 2022, 37(2):105-109, 172.
[3] VASUDEVA K, DUTTA A, MUNSHI A. Role of LncRNAs in the development of ischemic stroke and their therapeutic potential [J]. Molecular Neurobiology, 2021, 58(8): 3712-3728.
[4] PAN Yongli, JIAO Qingzheng, WEI Wei, et al. Emerging role of LncRNAs in ischemic stroke-novel insights into the regulation of inflammation [J]. Journal of Inflammation Research, 2021, 14: 4467-4483.
[5] MA Qingjie, DAI Xiaolin, LU Weiwei, et al. Silencing long non-coding RNA MEG8 inhibits the proliferation and induces the ferroptosis of hemangioma endothelial cells by regulating miR-497-5p/NOTCH2 axis [J]. Biochemical and Biophysical Research Communications, 2021, 556: 72-78.
[6] GHAFOURI-FARD S, KHOSHBAKHT T, HUSSEN B M, et al. A review on the role of MEG8 lncRNA in human disorders [J]. Cancer Cell Int, 2022, 22(1):285.
[7] 王志宏, 韩仲岩. 急性缺血性脑卒中的TOAST 分型标准[J]. 临床神经病学杂志,2007, 20(5):392. WANG Zh ihong, HAN Zhongyan. TOAST classification criteria for acute ischemic stroke[J]. Journal of Clinical Neurology, 2007, 20(5): 392.
[8] XU Shenbin, LU Jianan, SHAO Anwen, et al. Glial cells: role of the immune response in ischemic stroke [J]. Frontiers in Immunology, 2020, 11： 294.
[9] 孙丽坤, 赵凤依, 常玮鑫, 等. 急性缺血性脑卒中患者血清LncRNA SOX2OT 和miR-331 水平对不稳定斑块形成和重度神经功能缺损的诊断价值[J]. 现代检验医学杂志, 2022, 37(6)：76-82. SUN Likun, ZHAO Fengyi, CHANG Weixin, et al. Diagnostic value of serum LncRNA SOX2OT, miR-331 levels in patients with acute ischemic stroke for unstable plaque formation and severe neurological deficit [J]. Journal of Modern Laboratory Medicine, 2022, 37(6)：76-82.
[10] ZUO Lei, ZHANG Lin, ZU Juan, et al. Circulating circular RNAs as biomarkers for the diagnosis and prediction of outcomes in acute ischemic stroke[J]. Stroke, 2020, 51(1): 319-323.
[11] 范艳平, 易甲其, 吴敏. 缺血性脑卒中患者血清Hcy和EPO 水平的变化及其与颈动脉粥样硬化关系[J].现代检验医学杂志, 2017, 32(1)：80-83. FAN Yanping, YI Jiaqi, WU Min. Changes of serum Hcy and EPO levels in patients with ischemic stroke and their relationship with carotid artery atherosclerosis[J]. Journal of Modern Laboratory Medicine, 2017, 32(1): 80-83.
[12] GAN Li, LIAO Shengtao, XING Yu, et al. The regulatory functions of lncRNAs on angiogenesis following ischemic stroke[J]. Frontiers in Molecular Neuroscience, 2021, 13: 613976.
[13] YAN Yangtian, CHEN Li, ZHOU Jiajun, et al. SNHG12 inhibits oxygen-glucose deprivation-induced neuronal apoptosis via the miR-181a-5p/NEGR1 axis[J]. Molecular Medicine Reports, 2020, 22(5): 3886-3894.
[14] HUANG Jiao, YANG Jialei, LI Jinhong, et al. Association of long noncoding RNA H19 polymorphisms with the susceptibility and clinical features of ischemic stroke in southern Chinese Han population [J]. Metabolic Brain Disease, 2019, 34(4): 1011-1021.
[15] ZHANG Boya, DONG Yang, ZHAO Zhuo. LncRNA MEG8 regulates vascular smooth muscle cell proliferation, migration and apoptosis by targeting PPARα[J]. Biochemical and Biophysical Research Communications, 2019, 510(1): 171-176.
[16] SUI Shihua, SUN Lei, ZHANG Wenjing, et al. LncRNA MEG8 attenuates cerebral ischemia after ischemic stroke through targeting miR-130a-5p/VEGFA signaling [J]. Cellular and Molecular Neurobiology, 2021, 41(6): 1311-1324.
[17] CHALOS V, VAN DER ENDE N A M, LINGSMA H F, et al. National institutes of health stroke scale: an alternative primary outcome measure for trials of acute treatment for ischemic stroke [J]. Stroke, 2020, 51(1): 282-290.
[18] 张亚杰, 王玉琳, 董晓娇, 等. 急性缺血性脑卒中患者血清LncRNA SNHG12 表达水平检测及临床意义[J]. 现代检验医学杂志,2022,37(2):121-125. ZHAO Yajie, WANG Yulin, DONG Xiaojiao, et al. Detection of serum LncRNA SNHG12 expression level in patients with acute ischemic stroke and its clinical significance [J]. Journal of Modern Laboratory Medicine, 2022, 37(2): 121-125.
[19] HU?ANU A, IANCU M, B?LA?A R, et al. Predicting functional outcome of ischemic stroke patients in Romania based on plasma CRP, sTNFR-1, D-Dimers, NGAL and NSE measured using a biochip array [J]. Acta Pharmacol Sin, 2018, 39(7): 1228-1236.
[20] YU Bo, YANG Ping, XU Xuebi, et al. C-reactive protein for predicting all-cause mortality in patients with acute ischemic stroke: a meta-analysis [J]. Bioscience Reports, 2019, 39(2): BSR20181135.
[21] 赵玉君, 覃亮, 王宁, 等. 缺血性进展性脑卒中患者不同治疗方法对血液hs-CRP 和D-D 水平的影响[J]. 现代检验医学杂志, 2020, 35(5);142-146. ZHAO Yujun, QIN Liang, WANG Ning, et al. Effect of different treatment methods on the levels of hs-CRP and D-D in patients with progressive ischemic stroke [J]. Journal of Modern Laboratory Medicine, 2020, 35(5):142-146.
[22] ZHANG Wenjie, CAO Dongmei, WANG Ying, et al. LncRNA MEG8 is upregulated in gestational diabetes mellitus (GDM) and predicted kidney injury [J]. Journal of Diabetes and Its Complications, 2021, 35(1):107749.

相似文献/References:

[1]马 炜,朱 俊,徐 健,等.NLR与缺血性脑卒中患者预后关系研究[J].现代检验医学杂志,2016,31(04):139.[doi:10.3969/j.issn.1671-7414.2016.04.043]
　MA Wei,ZHU Jun,XU Jian,et al.Neutrophil to Lymphocyte Ratio Predicts Poor Prognosis for Acute Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2016,31(04):139.[doi:10.3969/j.issn.1671-7414.2016.04.043]
[2]邓 凯,许 薇,李 燕.缺血性脑卒中患者血浆vWF:Ag和S100B检测的临床意义[J].现代检验医学杂志,2017,32(05):52.[doi:10.3969/j.issn.1671-7414.2017.05.014]
　DENG Kai,XU Wei,LI Yan.Clinical Application of Detection of Plasma vWF:Ag and S100B in Ischemic Cerebral Apoplexy Disease[J].Journal of Modern Laboratory Medicine,2017,32(04):52.[doi:10.3969/j.issn.1671-7414.2017.05.014]
[3]张 艳a,刘 翔a,李鸿钏a,等.血清脂蛋白磷脂酶A2 和血浆抗凝血酶Ⅲ对2 型糖尿病患者发生缺血性脑卒中风险的评估价值[J].现代检验医学杂志,2020,35(04):71.[doi:10.3969/j.issn.1671-7414.2020.04.017]
　ZHANG Yana,LIU Xianga,LI Hong-chuana,et al.Evaluation Value of Serum Lipoprotein-Associated Phospholipase A2 and Plasma Antithrombin Ⅲ on the Risk of Ischemic Stroke in Patients with Type 2 Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2020,35(04):71.[doi:10.3969/j.issn.1671-7414.2020.04.017]
[4]利荣乔.血清Galectin-3和HDL-C水平检测对急性缺血性脑卒中预后的影响研究[J].现代检验医学杂志,2021,36(05):128.[doi:10.3969/j.issn.1671-7414.2021.05.029]
　LI Rong-qiao.Effect of Serum Galectin-3 and HDL-C Levels on Prognosis of AcuteIschemic Stroke[J].Journal of Modern Laboratory Medicine,2021,36(04):128.[doi:10.3969/j.issn.1671-7414.2021.05.029]
[5]杜龙庭,勿坡巫且,许春梅,等.缺血性脑卒中患者血清miR-155和PDCD4mRNA表达水平与颈动脉支架成形术后再狭窄的相关性研究[J].现代检验医学杂志,2022,37(02):105.[doi:10.3969/j.issn.1671-7414.2022.02.022]
　DU Long-ting,WUPO Wu-qie,XU Chun-mei,et al.Correlation between the Expression of miR-155, PDCD4 mRNA and Restenosis after Carotid Artery Stenting in Patients with Cerebral Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2022,37(04):105.[doi:10.3969/j.issn.1671-7414.2022.02.022]
[6]何 涛a,孔德强a,韩雪飞a,等.缺血性脑卒中患者行颈动脉支架成形术前血清CCR5，SDF-1 mRNA 表达水平与术后支架内再狭窄的相关性研究[J].现代检验医学杂志,2022,37(06):171.[doi:10.3969/j.issn.1671-7414.2022.06.033]
　HE Taoa,KONG De-qianga,HAN Xue-feia,et al.Correlation between Serum CCR5, SDF-1 mRNA Expression Levels and Postoperative In-stent Restenosis in Patients with Ischemic Stroke before Carotid Artery Stenting[J].Journal of Modern Laboratory Medicine,2022,37(04):171.[doi:10.3969/j.issn.1671-7414.2022.06.033]
[7]高继英a,石代乐b,王 刚c.缺血性脑卒中患者血清LncRNA SNHG8 的表达及临床意义[J].现代检验医学杂志,2023,38(05):75.[doi:10.3969/j.issn.1671-7414.2023.05.014]
　GAO Jiyinga,SHI Daileb,WANG Gangc.Expression and Clinical Significance of Serum LncRNA SNHG8 in Patients with Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2023,38(04):75.[doi:10.3969/j.issn.1671-7414.2023.05.014]
[8]李 芳,冉向阳,李 玮,等.缺血性脑卒中患者血清GR，SOD，Cys-C，Hcy和Lp（a）水平对预后的预警价值研究[J].现代检验医学杂志,2025,40(02):92.[doi:10.3969/j.issn.1671-7414.2025.02.017]
　LI Fang,RAN Xiangyang,LI Wei,et al.Study on the Prognostic Warning Value of Serum GR, SOD, Cys-C, Hcy and Lp(a) Levels in Patients with Cerebral Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2025,40(04):92.[doi:10.3969/j.issn.1671-7414.2025.02.017]

备注/Memo

备注/Memo:
基金项目： 2020 年度海南省卫生健康行业科研项目（20A200326）：极低频电磁场康复对缺血性脑卒中患者神经功能缺失恢复的作用及其机制研究。
作者简介：王桂瑜（1978-），女，本科，主治医师，研究方向：老年病，E-mail：wanggyu1978@163.com。
更新日期/Last Update: 2023-07-15