[1]孙 莉,马 艳,厉小梅,等.宏基因组二代测序对结缔组织病并发肺部感染的病原学诊断价值研究[J].现代检验医学杂志,2023,38(05):1-4,52.[doi:10.3969/j.issn.1671-7414.2023.05.001]
　SUN Li,MA Yan,LI Xiaomei,et al.Value of Metagenomic Next-generation Sequencing in the Pathogenic Diagnosis of Connective Tissue Disease Combined with Pulmonary Infection[J].Journal of Modern Laboratory Medicine,2023,38(05):1-4,52.[doi:10.3969/j.issn.1671-7414.2023.05.001]
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宏基因组二代测序对结缔组织病并发肺部感染的病原学诊断价值研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
1-4,52
栏目:
论著
出版日期:
2023-09-15

文章信息/Info

Title:
Value of Metagenomic Next-generation Sequencing in the Pathogenic Diagnosis of Connective Tissue Disease Combined with Pulmonary Infection
文章编号:
1671-7414（2023）05-001-05
作者:
孙 莉，马 艳，厉小梅，王 俐
（中国科学技术大学附属第一医院风湿免疫科，合肥 230000）
Author(s):
SUN Li, MA Yan, LI Xiaomei, WANG Li
（Department of Rheumatology and Immunology, the First Affiliated Hospital of University of Science and Technology of China, Hefei 230000, China）
关键词:
宏基因组二代测序; 病原学; 肺部感染; 结缔组织病
分类号:
Q781；R563.1
DOI:
10.3969/j.issn.1671-7414.2023.05.001
文献标志码:
A
摘要:
目的 研究宏基因组二代测序（metagenomic next-generation sequencing，mNGS）对结缔组织病并发肺部感染的病原学诊断价值。方法 选取2019 年5 月～ 2022 年5 月中国科学技术大学附属第一医院风湿免疫科诊治的结缔组织病并发肺部感染患者73 例，分析mNGS 与传统病原学检测的检出率、检测时间、病原体种类、各类病原体检出率。将患者分为重症肺炎组（n=44）和轻症肺炎组（n=29），比较两组病原体检出率及分布情况。结果 mNGS 病原体检出率（83.56%）、病毒（60.27%）、真菌（52.05%）和2 种及以上病原体（53.42%）检出率均高于传统检测（57.53%，17.81%，34.25%，13.70%），检测时间[25（5）h] 少于传统病原学检测[（120（48）h）]，差异均有统计学意义（χ2=10.452，23.077，4.966，22.400，Z=-10.155，P=0.001，0.001，0.026，<0.001，<0.001）。重症肺炎组mNGS（95.45%）和传统病原学（68.18%）检出率均高于轻症肺炎组（65.52%，41.38%），差异均有统计学意义（χ2=11.608，5.139；P=0.001，0.023）。结论 结缔组织病并发肺部感染患者中，mNGS 检测时间更短，病原体检出率高于传统检测，其有助于快速全面地检测和鉴定病原体，明确感染诊断和指导临床治疗。
Abstract:
Objective To investigate the value of metagenomic next-generation sequencing (mNGS) in the etiological diagnosis of connective tissue disease complicated with pulmonary infection. Methods A total of 73 patients with connective tissue disease combined with pulmonary infection diagnosed and treated by the Rheumatology Department of the First Affiliated Hospital of University of Science and Technology of China from May 2019 to May 2022 were selected to analyze the detection rate, detection time, pathogen type and detection rate of various pathogens between mNGS and traditional etiological detection. The patients were divided into severe pneumonia group（n=44）and mild pneumonia group （n=29）, and the detection rate and distribution of pathogens in the two groups were compared. Results The detection rate of mNGS pathogens (83.56%), viruses (60.27%), fungi (52.05%) and 2 or more pathogens (53.42%) were higher than traditional detection（57.53%，17.81%， 34.25%，13.70%），and the detection time [25 (5) h] was shorter than traditional detection[（120（48）h]，and the differences were statistically significant（χ2=10.452, 23.077, 4.966, 22.400，Z=-10.155, P=0.001, 0.001, 0.026, <0.001，<0.001）. The detection rates of mNGS (95.45%) and traditional etiology (68.18%) in severe pneumonia group were higher than those in mild pneumonia group（65.52%，41.38%），and the differences were statistically significant（χ2=11.608, 5.139, P=0.001，0.023）. Conclusion In patients with connective tissue disease complicated with pulmonary infection, the detection time of mNGS was shorter, and the pathogen detection rate was higher than that of traditional detection, which is helpful for rapid and comprehensive detection and identification of pathogens, clear infection diagnosis, and guide clinical treatment.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目（项目编号：81871271）：组织原位记忆T 细胞在干燥综合征中的作用及其分子调控机制的研究。
作者简介： 孙莉（1992-），女，硕士，住院医师，研究方向：风湿免疫系统相关疾病，E-mail: 18940134897@163.com。
通讯作者：王俐（1980-），女，博士，副主任医师，研究方向：风湿免疫系统相关疾病，E-mail：wang_liy@aliyun.com。
更新日期/Last Update: 2023-09-15