[1]刁 迅,范绮雨,耿良栋,等.基于生物信息学分析双硫死亡相关基因PDLIM1 mRNA在多种肿瘤中的表达及临床应用价值[J].现代检验医学杂志,2024,39(01):36-42+54.[doi:10.3969/j.issn.1671-7414.2024.01.007]
 DIAO Xun,FAN Qiyu,GENG Liangdong,et al.Analysis of Expression in Disulfidptosis-Related Gene PDLIM1 mRNA in Various Tumors and Its Clinical Application Value Based on Bioinformatics[J].Journal of Modern Laboratory Medicine,2024,39(01):36-42+54.[doi:10.3969/j.issn.1671-7414.2024.01.007]
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基于生物信息学分析双硫死亡相关基因PDLIM1 mRNA在多种肿瘤中的表达及临床应用价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年01期
页码:
36-42+54
栏目:
论著
出版日期:
2024-01-15

文章信息/Info

Title:
Analysis of Expression in Disulfidptosis-Related Gene PDLIM1 mRNA in Various Tumors and Its Clinical Application Value Based on Bioinformatics
文章编号:
1671-7414(2024)01-036-08
作者:
刁 迅1范绮雨1耿良栋2刘继斌1朱卫华1
(1. 南通大学附属肿瘤医院,江苏南通 226006;2. 南通大学附属医院普外科,江苏南通 226001)
Author(s):
DIAO Xun1 FAN Qiyu1 GENG Liangdong2 LIU Jibin1 ZHU Weihua1
(1. Affiliated Tumor Hospital of Nantong University, Jiangsu Nantong 226006, China; 2. Department of General Surgery, Affiliated Hospital of Nantong University, Jiangsu Nantong 226001, China)
关键词:
生物信息学PDZ 和LIM 域蛋白1双硫死亡肿瘤免疫浸润
分类号:
R730.43
DOI:
10.3969/j.issn.1671-7414.2024.01.007
文献标志码:
A
摘要:
目的 分析双硫死亡(disulfidptosis) 相关基因人PDZ 和LIM 域蛋白1(PDZ and LIM domain protein 1,PDLIM1)mRNA 在多种肿瘤中的表达及作用。方法 通过仙桃学术网站分析PDLIM1 mRNA 的表达情况。利用仙桃学术网站和Sangerbox 3.0 数据分析平台探究PDLIM1 在33 种肿瘤中的诊断和预后能力。利用TISIDB 数据库分析PDLIM1 与临床分级和分期的相关性。在Sangerbox 3.0 数据分析平台和Kaplan-Meier Plotter 数据库中分析PDLIM1 与肿瘤免疫相关性。通过STRING 数据库和Cytoscape 构建蛋白质互作网络(protein-protein interaction networks,PPI)。利用Sangerbox 3.0 数据分析平台进行富集分析。最后利用GSCA(Gene Set Cancer Analysis)网站分析获得PDLIM1 mRNA 表达与药物的敏感性。结果 PDLIM1 mRNA 在33 种肿瘤中表达量存在异质性。PDLIM1 在胆管癌(CHOL)、多形性胶质母细胞瘤(GBM)、肾透明细胞癌(KIRC)、肺腺癌(LUAD)、卵巢癌(OV)、胰腺癌(PAAD)、黑色素瘤(SKCM)和睾丸生殖细胞肿瘤(TGCT)中具有良好的诊断能力。PDLIM1 在胶质瘤(GBMLGG)、脑低级别胶质瘤(LGG)、混合肾癌(KIPAN)、多形性胶质细胞瘤(GBM)、间皮瘤(MESO)、葡萄膜黑色素瘤(UVM)和肾上腺皮质癌(ACC)中高表达预后差,而在肉瘤中低表达预后差。PDLIM1 mRNA 表达与头颈鳞状细胞癌(HIVSC)、肾乳头状细胞癌(KIRP)、子宫内膜癌(UCEC)、子宫癌肉瘤(UCS)和葡萄膜黑色素瘤(UVM)的分级,以及与宫颈癌、头颈鳞状细胞癌、子宫内膜癌和脑低级别胶质瘤肿瘤的分期有关。PDLIM1 与以前列腺癌为首的36 种肿瘤的免疫浸润显著相关,且发现在PDLIM1 mRNA 高表达的患者中经免疫治疗后的预后相对较好。PDLIM1在生物体内主要通过参与肌动蛋白细胞骨架、细胞黏附、肿瘤相关途径的调节来发挥作用,对以Isoliquiritigenin 为首的多种药物敏感。结论 PDLIM1 与多种肿瘤的临床预后和免疫浸润等方面密切相关,有望成为一种肿瘤诊断和预后生物标志物或治疗靶点。
Abstract:
Objective To analyze the expression and role of the disulfidptosis-related gene PDZ and LIM domain protein 1 (PDLIM1) in various tumors. Methods The expression of PDLIM1 mRNA was analyzed by Xiantao website. The diagnostic and prognostic capabilities of PDLIM1 in 33 types of tumors were explored using the Xiantao website and Sangerbox 3.0 data analysis platform. The correlation between PDLIM1 and clinical classification and its staging was analyzed by the TISIDB database. The correlation between PDLIM1 and tumor immunity was analyzed by Sangerbox 3.0 data analysis platform and Kaplan-Meier Plotter database. Protein-protein interaction networks (PPI) were constructed by STRING database and Cytoscape, and were enriched by Sangerbox 3.0 data analysis platform. Finally, the GSCA website was applied to acquire the expression of PDLIM1 mRNA and its sensitivity to drugs. Results There was heterogeneity in the expression of PDLIM1 mRNA among 33 tumors. PDLIM1 had good diagnostic ability in cholangiocarcinoma (CHOL), glioblastoma multiforme (GBM), kidney renal clear cell carcinoma (KIRC), lung adenocarcinoma (LUAD), ovarian cancer (OV), pancreatic cancer (PAAD), skin cutaneous melanoma (SKCM) and testicular germ cell tumor (TGCT). High expression of PDLIM1 mRNA in glioma, low-grade glioma (LGG), KIPAN, GBM, uveal melanoma (UVM), and adrenocortical carcinoma (ACC) suggested poor prognosis, while low expression in sarcoma suggested poor prognosis. PALIM1 mRNA expression was correlated with the classification of head and neck squamous cell carcinoma (HNSC), kidney renal papillary cell carcinoma (KIRP), uterine corpus endometrial carcinoma (UCEC), uterine carcinosarcomas (UCS), and UVM as well as the staging of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), HNSC, UCEC, and LGG. PDLIM1 was significantly associated with immune infiltration of 36 tumors led by prostateadenocarcinoma (PRAD), and was found to have a relatively good prognosis after immunotherapy in patients with high PDLIM1 mRNA expression. PDLIM1 exerted effects on organisms mainly through its involvement in the regulation of actin cytoskeleton, cell adhesion, and cancer-related pathways, and was sensitive to various drugs led by Isoliquiritigenin. Conclusion  PDLIM1 was closely related to the clinical prognosis and immune infiltration of a variety of tumors, and it is expected to be a cancer diagnostic and prognostic biomarker or therapeutic target.

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备注/Memo

备注/Memo:
基金项目:南通市科技局计划项目(JC22022027),江苏省研究生科研与实践创新计划项目(SJCX22-1637)。
作者简介: 刁迅(1998-),女,硕士,研究方向:临床检验诊断学,E-mail:1978852793@qq.com。
通讯作者:朱卫华:E-mail:1585543600@qq.com,Tel:0513-86712016。
更新日期/Last Update: 2024-01-15