[1]吴迎爽a,薛向东a,杜志辉a,等.特发性矮小症患儿血清Vaspin和SFRP5水平表达及其诊断价值研究[J].现代检验医学杂志,2024,39(01):95-99.[doi:10.3969/j.issn.1671-7414.2024.01.017]
　WU Yingshuanga,XUE Xiangdonga,DU Zhihuia,et al.Expression of Serum Vaspin and SFRP5 in Children with Idiopathic Nanosomia and Their Diagnostic Value[J].Journal of Modern Laboratory Medicine,2024,39(01):95-99.[doi:10.3969/j.issn.1671-7414.2024.01.017]
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特发性矮小症患儿血清Vaspin和SFRP5水平表达及其诊断价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年01期
页码:
95-99
栏目:
论著
出版日期:
2024-01-15

文章信息/Info

Title:
Expression of Serum Vaspin and SFRP5 in Children with Idiopathic Nanosomia and Their Diagnostic Value
文章编号:
1671-7414（2024）01-095-05
作者:
吴迎爽a; 薛向东a; 杜志辉a; 王利红a; 程渊博a; 牛晓庆a; 王大伟b
（张家口市第一医院a. 儿科；b. 骨二科，河北张家口 075000）
Author(s):
WU Yingshuanga;  XUE Xiangdonga;  DU Zhihuia;  WANG Lihonga;  CHENG Yuanboa;  NIU Xiaoqinga;  WANG Daweib
（a. Department of Pediatrics；b. Department of Orthopaedics，the First Hospital of Zhangjiakou, Hebei Zhangjiakou 075000，China）
关键词:
特发性矮小症; 内脏脂肪特异性丝氨酸蛋白酶抑制剂; 分泌型卷曲相关蛋白5
分类号:
R725.8；R446.11
DOI:
10.3969/j.issn.1671-7414.2024.01.017
文献标志码:
A
摘要:
目的　探索特发性矮小症（idiopathic short stature，ISS）患儿血清中内脏脂肪特异性丝氨酸蛋白酶抑制剂（visceraladipose tissue-derived serine protease inhibitor, Vaspin） 和分泌型卷曲相关蛋白5（secreted frizzled-related protein5，SFRP5）的表达水平及其诊断价值。方法　选取2021 年12 月～ 2023 年2 月在张家口市第一医院确诊的70 例ISS 患儿作为疾病组，同期选择72 例体检健康的志愿儿童作为对照组。采用免疫发光法检测Vaspin 的表达水平，酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）检测SFRP5 的表达水平，对两组儿童的临床资料进行分析。受试者工作特征（receiver operator characteristics curve，ROC）曲线分析血清Vaspin 和SFRP5 对ISS 的诊断价值，多因素Logistic 回归分析ISS 发生的影响因素。结果　与对照组比较，疾病组的血清Vaspin 水平显著升高（2.89±0.92 ng/ml vs1.81±0.42 ng/ml），SFRP5 水平显著降低（10.22±2.84 pg/ml vs 13.21±3.53 pg/ml），差异具有统计学意义（t=9.040，5.552，均P<0.05）。疾病组儿童的体重、身高、身体质量指数（body mass index ，BMI）、性发育状态Ⅱ～Ⅴ期比例显著低于对照组儿童，差异具有统计学意义（t=7.687，6.330，5.559，7.074，均P<0.05）；ROC 曲线下面积（AUC）显示，Vaspin，SFRP5 及二者联合检测诊断ISS 患儿的AUC 分别为0.768，0.849 和0.925，二者联合诊断效能优于血清Vaspin，SFRP5 各自单独诊断（Z=3.829，P ＜ 0.001；Z=2.141，P=0.032）。多因素Logistic 回归分析显示BMI（OR=0.508，95%CI=0.260 ～ 0.991），Vaspin（OR=3.458，95%CI：1.125 ～ 10.631），SFRP5（OR=0.378，95%CI：0.153 ～ 0.935）是ISS 的影响因素（均P ＜ 0.05）。结论　ISS 患儿血清中Vaspin 表达水平显著升高，SFRP5 表达水平显著降低，二者是ISS 发生的影响因素，二者联合检测对ISS 的诊断具有一定的价值。
Abstract:
Objective　To explore the expression level of visceral adipose tissue-derived serine protease inhibitor(Vaspin) and secreted frizzled-related protein5(SFRP5) in the serum of children with idiopathic short stature (ISS) and its diagnostic value. Methods　70 children with ISS diagnosed in the First Hospital of Zhangjiakou from December 2021 to February 2023 were selected as the disease group, while 72 healthy volunteer children who underwent physical examination were collected as the control group. Immunoluminescence was applied to detect the expression level of VASPIN, Enzyme-linked immunosorbent assay （ELISA）was applied to detect the expression level of SFRP5 the clinical data of children in two groups were analyzed. Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of serum Vaspin and SFRP5 for ISS, multivariate Logistic regression was used to analyze the influencing factors of ISS. Results　Compared with the control group, the serum Vaspin level in the disease group was obviously increased(2.89±0.92 ng/ml vs 1.81±0.42 ng/ml), while the SFRP5 level was obviously reduced(10.22±2.84 pg/ml vs 13.21±3.53 pg/ml), the differences were statistically significant (t=9.040, 5.552, all P<0.05). The weight, height, body mass index（BMI） and proportion of sexual development stage II ～ V of children in the disease group were obviously lower than those in the control group, and the differences were statistically significant(t=7.687, 6.330, 5.559, 7.024, all P<0.05). The area under ROC curve showed that the AUC of Vaspin and SFRP5 and their combined detection in the diagnosis of ISS were 0.768, 0.849 and 0.925, respectively, the combined diagnosis efficacy of Vaspin and SFRP5 was better than that of serum Vaspin and SFRP5 alone (Z =3.829, P<0.001；Z =2.141, P=0.032). Multivariate Logistic regression analysis showed that BMI(OR=0.508, 95%CI：0.260 ～ 0.991), Vaspin(OR=3.458, 95%CI：1.125 ～ 10.631) and SFRP5(OR=0.378, 95%CI：0.153 ～ 0.935) were the influencing factors for ISS (all P<0.05). Conclusion　The expression level of Vaspin in the serum of children with ISS is obviously increased, while the expression level of SFRP5 is obviously reduced. The two are influencing factors of ISS, and the combined detection of their expression levels has certain value in the diagnosis of ISS.

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备注/Memo

备注/Memo:
基金项目： 张家口市重点研发项目（编号1921085D）：浆细胞瘤多样性异位基因1 在小儿激素敏感型和抵抗型支气管哮喘中的表达及作用机制研究。
作者简介：吴迎爽（1983-），女，硕士，副主任医师，研究方向：小儿矮小症，E-mail：u072v1@163.com。
通讯作者：王大伟（1982-），男，硕士，副主任医师，研究方向：小儿矮小症颈腰椎相关疾病，E-mail：pucengtnw08@163.com 。
更新日期/Last Update: 2024-01-15