[1]周婷婷,沈一沁,孙晓帆.变应性支气管肺曲霉菌病患者血清Periostin，IL-5，IL-8和IL-13表达及其与肺功能相关性研究[J].现代检验医学杂志,2025,40(01):48-52+68.[doi:10.3969/j.issn.1671-7414.2025.01.009]
　ZHOU Tingting,SHEN Yiqin,SUN Xiaofan.Study on the Correlation between Serum Periostin, IL-5, IL-8,IL-13 levels and Lung Function in Patients with Allergic Bronchopulmonary Aspergillosis[J].Journal of Modern Laboratory Medicine,2025,40(01):48-52+68.[doi:10.3969/j.issn.1671-7414.2025.01.009]
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变应性支气管肺曲霉菌病患者血清Periostin，IL-5，IL-8和IL-13表达及其与肺功能相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年01期
页码:
48-52+68
栏目:
论著
出版日期:
2025-01-15

文章信息/Info

Title:
Study on the Correlation between Serum Periostin, IL-5, IL-8,IL-13 levels and Lung Function in Patients with Allergic Bronchopulmonary Aspergillosis
文章编号:
1671-7414（2025）01-048-06
作者:
周婷婷1; 沈一沁2; 孙晓帆1
（1. 上海交通大学医学院附属新华医院呼吸内科，上海 200082；2. 复旦大学附属华山医院检验科，上海 200040）
Author(s):
ZHOU Tingting1; SHEN Yiqin2; SUN Xiaofan1
(1. Department of Respiratory Medicine, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine , Shanghai 200082, China；2. Department of Clinical Laboratory， Huashan Hospital Affiliated to Fudan University，Shanghai 200040，China)
关键词:
变应性支气管肺曲霉菌病; 骨膜蛋白; 白细胞介素-5; 白细胞介素-8; 白细胞介素-13; 肺功能
分类号:
R379；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.01.009
文献标志码:
A
摘要:
目的 探讨变应性支气管肺曲霉菌病（ABPA）患者血清骨膜蛋白（Periostin）、IL-5，IL-8 和IL-13 水平表达及其与肺功能的相关性。方法 选取上海交通大学医学院附属新华医院2019 年1 月～ 2022 年12 月收治的73 例ABPA患者为病例组，随机选取同期的60 例哮喘患者为对照组。采用酶联免疫吸附法（ELISA）检测血清Periostin，IL-5，IL-8 和IL-13 水平；Pearson 积矩相关分析血清Periostin 与IL-5，IL-8，IL-13，肺功能的相关性；多因素Logistic 回归分析血清Periostin 与支气管哮喘患者ABPA 的关系；受试者工作特征（ROC）曲线评估血清Periostin 对ABPA 的诊断价值。结果 与对照组比较，病例组血清Periostin（97.64±28.05 ng/ml vs 57.39±22.78 ng/ml），IL-5（1.62±0.35 ng/L vs 0.59±0.31 ng/L），IL-8（79.22±10.26 ng/L vs 51.04±8.26 ng/L），IL-13（1.59±0.43 ng/L vs 1.02±0.51 ng/L）水平显著升高（t=6.997 ～ 17.776）；血清总IgE、特异性IgE 阳性率、嗜酸性粒细胞计数、呼出气一氧化氮（FeNO）水平升高（t=9.341 ～ 131.469）、第1 秒用力呼气容积占预计值百分比（FEV1%pred）、第1 秒用力呼气容积/ 用力肺活量比值（FEV1/FVC）水平降低（t=2.652，3.126），差异具有统计学意义（均P<0.05）。ABPA 患者血清Periostin 与IL-5，IL-8，IL-13，FeNO 呈正相关（r=0.539 ～ 0.695，均P<0.05），与FEV1%pred，FVC%pred，FEV1/FVC 呈负相关（r=-0.657，-0.506，-0.582，均P<0.05）。与血清Periostin<68.35ng/ml 比较，血清Periostin 97.83 ～ 131.02 ng/ml，>131.02 ng/ml 的支气管哮喘患者ABPA 发生风险更高，差异具有统计学意义（均P<0.05）。血清Periostin+IL-5+IL-8+IL-13+ 总IgE+ 特异性IgE+ 嗜酸性粒细胞联合诊断ABPA 的AUC（95%CI）优于单纯Periostin 及Periostin 联合炎症因子诊断，差异具有统计学意义（Z=3.562，2.931，均P<0.05）。结论 ABPA 患者血清Periostin 异常升高，并与肺功能密切相关，早期检测可辅助临床诊断ABPA。
Abstract:
Objective To explore serum periostein, interleukin (IL) -5, IL-8 and IL-13 expression levels in patients with allergic bronchopulmonary aspergillosis (ABPA) and their correlation with lung function. Methods 73 patients with ABPA admitted to Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine from January 2019 to December 2022 were selected as the case group, and 60 asthma patients during the same period were selected as the control group. ELISA was used to detect serum levels of Periodin, IL-5, IL-8 and IL-13. Pearson analysis was used to investigate the correlation between serum Periodin and lung function indicators. Multivariate Logistic regression analysis was used to investigate the effect of serum period on the occurrence of ABPA in patients with bronchial asthma, and the diagnostic value of serum Periodin for ABPA was evaluated by ROC curves. Results Compared with the control group, the serum levels of Periostin（97.64±28.05 ng/ml vs 57.39±22.78 ng/ml），IL-5（1.62±0.35 ng/L vs 0.59±0.31 ng/L），IL-8（79.22±10.26 ng/L vs 51.04±8.26 ng/L ）and IL-13（1.59±0.43 ng/L vs 1.02±0.51 ng/L）in the case group increased（t=6.997 ～ 17.776), the total serum IgE, specific IgE positivity rate, eosinophil count and fractional exhaled nitric oxide (FeNO) levels in the case group increased(t=9.341 ～131.469), the percentage of forced expiratory volume in the first second to the expected value (FEV1%pred), and forced expiratory volume in the first second/forced vital capacity (FEV1/FVC) levels decreased(t=2.652, 3.126),the differences were statistically significant(all P<0.05), respectively. Serum Periodin in ABPA patients was positively correlated with IL-5,IL-8, IL-13 and FeNO (r=0.539 ～ 0.695，all P<0.05), and negatively correlated with FEV1% pred, FVC% pred and FEV1/ FVC (r=-0.657，-0.506，-0.582，all P<0.05). Compared with serum Periosin ＞ 68.35 ng/ml, bronchial asthma patients with serum Periodin 97.83 ～ 131.02 ng/ml and ＞ 131.02 ng/ml had a higher risk of ABPA, and the differences were statistically significant (all P<0.05), respectively .The area under the curve of combined diagnosis of ABPA with serum Periodin+IL-5+IL- 8+IL-13+total IgE+specific IgE+eosinophils was better than that of single periodin and periodin combined with inflammatory factor diagnosis, the difference was statistically significant(Z=3.562, 2.931, all P<0.05).Conclusion Serum Periostin is abnormally elevated in ABPA patients, which is closely related to lung function. Early detection can assist in clinical diagnosis of ABPA.

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备注/Memo

备注/Memo:
基金项目：上海市卫生健康委员会卫生行业临床研究专项（2020325）。
作者简介：周婷婷(1990-)，女，本科，技师，研究方向：肺功能技术，E-mail：zhoutingting4564@126.com。
更新日期/Last Update: 2025-01-15