[1]许玫莎,王 聪,郑友峰,等.急性肺栓塞患者血浆TIMP-1，VEGF和LTBP-2水平表达与危险分层及死亡的相关性研究[J].现代检验医学杂志,2025,40(01):169-173.[doi:10.3969/j.issn.1671-7414.2025.01.032]
　XU Meisha,WANG Cong,ZHENG Youfeng,et al.Study on the Correlation between the Expression of Plasma TIMP-1，VEGF and LTBP-2 Levels and Risk Stratification and Mortality in Patients with Acute Pulmonary Embolism[J].Journal of Modern Laboratory Medicine,2025,40(01):169-173.[doi:10.3969/j.issn.1671-7414.2025.01.032]
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急性肺栓塞患者血浆TIMP-1，VEGF和LTBP-2水平表达与危险分层及死亡的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年01期
页码:
169-173
栏目:
论著
出版日期:
2025-01-15

文章信息/Info

Title:
Study on the Correlation between the Expression of Plasma TIMP-1，VEGF and LTBP-2 Levels and Risk Stratification and Mortality in Patients with Acute Pulmonary Embolism
文章编号:
1671-7414（2025）01-169-05
作者:
许玫莎; 王 聪; 郑友峰; 吴挺实; 肖成钦; 陈 伟
（海口市第三人民医院呼吸内科，海口 571100）
Author(s):
XU Meisha; WANG Cong; ZHENG Youfeng; WU Tingshi; XIAO Chengqin; CHEN Wei
（Department of Respiratory，the Third People’s Hospital of Haikou，Haikou 571100，China）
关键词:
急性肺栓塞; 金属蛋白酶组织抑制因子-1; 血管内皮生长因子; 潜在转化生长因子结合蛋白 2; 危险分层; 死亡
分类号:
R563.5；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.01.032
文献标志码:
A
摘要:
目的 探讨急性肺栓塞（APE）患者血浆金属蛋白酶组织抑制因子-1（TIMP-1）、血管内皮生长因子（VEGF）及潜在转化生长因子结合蛋白2（LTBP-2）水平与危险分层和死亡的关系。方法 选取2022 年1 月～ 2024 年1 月海口市第三人民医院收治的APE 患者110 例进行危险分层，分为低危组（n=28）、中危组（n=43）和高危组（n=39）；根据APE 患者发生死亡情况分为存活组（n=79）和死亡组（n=31）。采用酶联免疫吸附法（ELISA）测定血浆TIMP-1，VEGF及LTBP-2 水平；应用多元Logistic 回归分析影响APE 患者死亡的危险因素，绘制ROC 曲线分析血浆TIMP-1，VEGF及LTBP-2 水平预测APE 患者死亡的价值；采用Pearson 相关分析血浆TIMP-1，VEGF 及LTBP-2 水平与APE 患者临床指标的相关性。结果 死亡组心率、B 型脑钠肽（BNP）、D- 二聚体、TIMP-1（207.15±62.84pg/ml vs 152.48±41.62pg/ml），VEGF（726.35±190.46pg/ml vs 419.27±115.28pg/ml）及LTBP-2（29.17±6.38ng/ml vs 13.26±3.70ng/ml）水平均明显高于存活组，差异具有统计学意义（t=5.386 ～ 21.194，均P<0.05）。高危组和中危组血浆TIMP-1（204.15±60.17 pg/ml，178.18±51.30pg/ml vs 148.20±34.80pg/ml），VEGF（720.83±204.18pg/ml，580.16±158.37pg/ml vs 412.15±109.26pg/ml）及LTBP-2（28.40±6.41ng/ml，21.37±5.26ng/ml vs 12.84±3.12ng/ml）水平均明显高于低危组（t=8.417 ～ 19.850），且高危组高于中危组（t=7.964，9.381，11.470），差异具有统计学意义（均P<0.05）。多元Logistic 回归分析显示，血浆BNP，D- 二聚体，TIMP-1，VEGF 及LTBP-2 水平升高是影响APE 患者死亡的危险因素（均P<0.05）。ROC 曲线分析显示，TIMP-1，VEGF 及LTBP-2 三项联合预测APE 患者死亡的AUC（95%CI）最大[0.938（0.881 ～ 0.997）]，其准确度为88.2%。Pearson相关分析显示，APE患者血浆TIMP-1，VEGF及LTBP-2水平与BNP及D-二聚体均呈正相关（r=0.416～0.753，均P<0.05）。结论 血浆TIMP-1，VEGF 及LTBP-2 水平升高与APE 高危和死亡有关，三项联合对APE 患者死亡具有较好的预测价值。
Abstract:
Objective To investigate the relationship between plasma metalloproteinase-tissue inhibitor 1 (TIMP-1)，vascular endothelial growth factor (VEGF) and potentially transforming growth factor binding protein 2 (LTBP-2) levels and risk stratification and death in patients with acute pulmonary embolism (APE). Methods A toral of 110 APE patients admitted to the Third People’s Hospital of Haikou from January 2022 to January 2024 were selected for risk stratification, and they were divided into low-risk group（n=28）, medium-risk group（n=43） and high-risk groups（n=39）. According to the occurrence of death in APE patients, they were divided into a survival group （n=79）and a death group（n=31）. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of plasma TIMP-1，VEGF and LTBP-2. Applying multiple Logistic regression analysis to identify risk factors affecting the mortality of APE patients and plotting ROC curve to analyze the predictive value of plasma TIMP-1，VEGF and LTBP-2 levels for APE patients mortality and. Pearson correlation analysis was used to analyze the correlation between plasma levels of TIMP-1，VEGF and LTBP-2 and clinical indexes in APE patients. Results The heart rate, B-type brain natriuretic peptide (BNP), D-dimer, TIMP-1（207.15±62.84pg/ml vs 152.48±41.62pg/ml）, VEGF （726.35±190.46pg/ml vs 419.27±115.28pg/ml） and LTBP-2（29.17±6.38ng/ml vs 13.26±3.70ng/ml） levels in the death group were significantly higher than those in the survival group, the difference were statistically significant (t=5.386 ～ 21.194, all P<0.05). The levels of plasma TIMP-1（204.15±60.17 pg/ml，178.18±51.30pg/ml vs 148.20±34.80pg/ ml）, VEGF（720.83±204.18pg/ml，580.16±158.37pg/ml vs 412.15±109.26pg/ml） and LTBP-2（28.40±6.41ng/ml， 21.37±5.26ng/ml vs 12.84±3.12ng/ml） in high-risk and medium-risk groups were significantly higher than those in the low-risk group（t=8.417 ～ 19.850）, and those in the high-risk group were higher than those in the medium-risk group（t=7.964，9.381， 11.470）, the differences were statistically significant (all P<0.05)，respectively. Multiple Logistic regression analysis showed that increased plasma BNP, D-dimer，TIMP-1，VEGF and LTBP-2 levels were risk factors for death in APE patients (all P<0.05). ROC curve analysis showed that TIMP-1, VEGF and LTBP-2 combined predicted the highest AUC（95%CI） of death in APE patients [0.938(0.881 ～ 0.997)], with an accuracy of 88.2%. Pearson correlation analysis showed that the levels of plasma TIMP-1, VEGF and LTBP-2 in APE patients were positively correlated with BNP and D-dimer (r=0.416 ～ 0.753, all P<0.05). Conclusion Elevated levels of plasma TIMP-1, VEGF and LTBP-2 are associated with high risk and mortality in APE, and the combination of these three factors has good predictive value for mortality in APE patients.

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备注/Memo

备注/Memo:
基金项目：海南省医药卫生科研基金项目（21A200156）。
作者简介：许玫莎（1982-）, 女，本科，主治医师，主要从事临床呼吸内科疾病研究，E-mail：m13876126309_2@163.com。
更新日期/Last Update: 2025-01-15