[1]耿保伟,彭古涛,汪 峰,等.创伤性脑损伤患者血清LncRNA HOTAIR和LncRNA HOXA11-AS水平表达及与认知功能的关系研究[J].现代检验医学杂志,2025,40(02):87-91.[doi:10.3969/j.issn.1671-7414.2025.02.016]
　GENG Baowei,PENG Gutao,WANG Feng,et al.Serum LncRNA HOTAIR and LncRNA HOXA11-AS Levels and Their Relationship with Cognitive Function in Patients with Traumatic Brain Injury[J].Journal of Modern Laboratory Medicine,2025,40(02):87-91.[doi:10.3969/j.issn.1671-7414.2025.02.016]
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创伤性脑损伤患者血清LncRNA HOTAIR和LncRNA HOXA11-AS水平表达及与认知功能的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
87-91
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Serum LncRNA HOTAIR and LncRNA HOXA11-AS Levels and Their Relationship with Cognitive Function in Patients with Traumatic Brain Injury
文章编号:
1671-7414（2025）02-087-05
作者:
耿保伟; 彭古涛; 汪 峰; 刘思江; 杨 帆; 汤厅均
（重庆大学附属涪陵医院神经外科，重庆 408000）
Author(s):
GENG Baowei; PENG Gutao; WANG Feng; LIU Sijiang;  YANG Fan;  TANG Tingjun
（Department of Neurosurgery，Fuling Hospital Affiliated to Chongqing University，Chongqing 408000，China）
关键词:
创伤性脑损伤; HOX 基因座转录本反义RNA; 同源异形盒基因A11 反义RNA; 炎症因子; 认知功能
分类号:
R651.15；Q786
DOI:
10.3969/j.issn.1671-7414.2025.02.016
文献标志码:
A
摘要:
目的 探讨创伤性脑损伤（TBI）患者血清长链非编码RNA（LncRNA）HOX 基因座转录本反义RNA（HOTAIR）和同源异形盒基因A11 反义RNA（LncRNA HOXA11-AS）水平及其与认知功能的关系。方法 选取2022 年1 月～ 2023年12 月重庆大学附属涪陵医院收治的106 例TBI 患者为TBI 组，根据其是否发生认知功能障碍分为认知障碍组（n=44）和无认知障碍组（n=62）；另选取同期该院78 例健康体检者为对照组。采用实时荧光定量PCR（qRT-PCR）法检测血清LncRNA HOTAIR 和LncRNA HOXA11-AS 水平；Spearman 及Pearson 法分析TBI 患者血清LncRNA HOTAIR，LncRNAHOXA11-AS 水平与认知功能和炎症因子的相关性；受试者工作特征（ROC）曲线分析血清LncRNA HOTAIR 和LncRNAHOXA11-AS 对TBI 患者发生认知功能障碍的预测价值；Logistic 回归分析血清LncRNA HOTAIR 和LncRNA HOXA11-AS表达对TBI 后认知障碍的影响。结果 与对照组相比，TBI 组血清LncRNA HOTAIR（1.75±0.29 vs 1.03±0.15）和LncRNAHOXA11-AS（1.59±0.35 vs 0.99±0.18）表达水平明显升高，差异具有统计学意义（t=20.034，13.846，均P ＜ 0.05）。认知障碍组血清LncRNA HOTAIR，LncRNA HOXA11-AS，肿瘤坏死因子-α（TNF-α），白细胞介素1β（IL-1β）和IL-6 水平明显高于无认知障碍组（t=3.011 ～ 9.615），蒙特利尔认知评估量表（MoCA）明显低于无认知障碍组（t=17.633），差异具有统计学意义（均P ＜ 0.05）。Spearman 相关分析，TBI 患者血清LncRNA HOTAIR，LncRNA HOXA11-AS 表达水平与MoCA 评分呈负相关（r=-0.515，-0.430，均P<0.001）；Pearson 相关分析，血清LncRNA HOTAIR，LncRNAHOXA11-AS 表达水平与TNF-α，IL-1β，IL-6 水平呈正相关（r=0.423，0.397，0.452，0.437，0.512，0.390， 均P<0.001）。血清LncRNA HOTAIR 和LncRNA HOXA11-AS 单独及联合预测TBI 后认知障碍的AUC（95%CI）分别为0.896（0.822 ～ 0.947），0.864（0.784 ～ 0.923），0.960（0.903 ～ 0.988），二者联合预测价值优于单独预测（Z=2.457，2.998，均P ＜ 0.05）。Logistic 回归分析血清LncRNA HOTAIR 和LncRNA HOXA11-AS 表达水平升高为TBII 患者发生认知障碍的危险因素，MoCA 评分为保护因素（均P ＜ 0.05）。结论 血清LncRNA HOTAIR 和LncRNA HOXA11-AS 水平对预测TBI 患者认知功能障碍具有一定价值，且其可能通过调节体内炎症因子水平参与TBI 患者认知功能障碍发生及进展。
Abstract:
Objective To investigate the serum levels of long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) and homeobox A11 antisense RNA (LncRNA HOXA11-AS) in patients with traumatic brain injury (TBI), and their relationship with cognitive function. Methods From January 2022 to December 2023, 106 TBI patients who visited Fuling Hospital Affiliated to Chongqing University were regarded as the TBI group. They were separated into a cognitive impairment group (n=44) and a non-cognitive impairment group (n=62) based on whether they experienced cognitive impairment. 78 healthy individuals who underwent physical examinations in Fuling Hospital Affiliated to Chongqing University were regarded as the control group. Real-time fluorescence quantitative PCR（qRT-DCR） method was applied to detect serum LncRNA HOTAIR and LncRNA HOXA11-AS levels. Spearman and Pearson methods were used to analyze the correlation between serum LncRNA HOTAIR and LncRNA HOXA11-AS levels, cognitive function, and inflammatory factors in TBI patients. Receiver operating characteristic (ROC) curve was applied to analyze the predictive value of serum LncRNA HOTAIR and LncRNA HOXA11-AS for cognitive impairment in TBI patients. Logistic regression was applied to analyze the impacts of serum LncRNA HOTAIR and LncRNA HOXA11-AS expression on cognitive impairment after TBI. Results Compared with the control group, the expression levels of serum LncRNA HOTAIR (1.75 ± 0.29 vs 1.03 ± 0.15) and LncRNA HOXA11-AS(1.59 ± 0.35 vs 0.99 ± 0.18) in the TBI group were significantly increased, and the differences were statistically significant (t=20.034, 13.846, all P<0.05). The cognitively impaired group had significantly higher serum LncRNA HOTAIR，LncRNA HOXA11-AS，tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6) levels were significantly higher in the cognitive impairment group than in the group without cognitive impairment (t=3.011 ～ 9.615), and Montreal Cognitive Assessment (MoCA) scale was considerably lower than that of the no cognitive impairment group (t=17.633)，and the differences were statistical significance（all P<0.05). Spearman correlation analysis showed that the expression levels of serum LncRNA HOTAIR and LncRNA HOXA11- AS in TBI patients were negatively correlated with MoCA scores (r=-0.515,-0.430, all P<0.001)，Pearson correlation analysis showed that the expression levels of serum LncRNA HOTAIR and LncRNA HOXA11-AS were positively correlated with TNF-α，IL-1βand IL-6 levels (r=0.423，0.397, 0.452, 0.437, 0.512, 0.390, all P<0.001). The AUC（95%CI）of serum LncRNA HOTAIR and LncRNA HOXA11-AS alone and in combination predicted cognitive impairment after TBI was 0.896 （0.822 ～ 0.947），0.864（0.784 ～ 0.923）and 0.960（0.903 ～ 0.988）, respectively, the combined predictive value of the two was better than that of individual prediction (Z=2.457, 2.998，all P<0.05). Logistic regression analysis showed that elevated expression levels of serum LncRNA HOTAIR and LncRNA HOXA11-AS were risk factors for cognitive impairment in TBII patients，while MoCA score was a protective factor (all P<0.05). Conclusion The levels of serum LncRNA HOTAIR and LncRNA HOXA11-AS have certain value in the functional impairment of TBI patients, and they may participate in the occurrence and progression of cognitive impairment in TBI patients by regulating the levels of inflammatory factors in the body.

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备注/Memo

备注/Memo:
基金项目：重庆市涪陵区科卫联合医学科研项目（编号：2023KWLH020）。
作者简介：耿保伟（1983-），男，硕士研究生，副主任医师，研究方向：神经外科基础与临床，E-mail：v58lus@163.com。
更新日期/Last Update: 2025-03-15