[1]张忠俊a,陈 琳b.下肢动脉硬化闭塞症患者血清miR-150和miR-155水平表达对支架植入术再狭窄的预测价值[J].现代检验医学杂志,2025,40(02):109-113.[doi:10.3969/j.issn.1671-7414.2025.02.020]
　ZHANG Zhongjuna,CHEN Linb.Predictive Value of Serum miR-150 and miR-155 Expression Levels in Patients with Lower Limb Arteriosclerosis Obliterans for Restenosis after Stent Implantation[J].Journal of Modern Laboratory Medicine,2025,40(02):109-113.[doi:10.3969/j.issn.1671-7414.2025.02.020]
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下肢动脉硬化闭塞症患者血清miR-150和miR-155水平表达对支架植入术再狭窄的预测价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
109-113
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Predictive Value of Serum miR-150 and miR-155 Expression Levels in Patients with Lower Limb Arteriosclerosis Obliterans for Restenosis after Stent Implantation
文章编号:
1671-7414（2025）02-109-05
作者:
张忠俊a; 陈 琳b
（德阳市人民医院a. 介入科；b. 放射科，四川德阳 618000）
Author(s):
ZHANG Zhongjuna; CHEN Linb
(a. Department of Interventional；b. Department of Radiology，Deyang People’s Hospital， Sichuan Deyang 618000，China)
关键词:
下肢动脉硬化闭塞症; 支架内再狭窄; 微小RNA-150; 微小RNA-155
分类号:
R543；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.02.020
文献标志码:
A
摘要:
目的 探究下肢动脉硬化闭塞症（ASO）患者血清miR-150 和miR-155 水平表达对支架植入术再狭窄（ISR）的预测价值。方法 选取2020 年3 月～ 2022 年6 月期间在德阳市人民医院接受支架植入治疗的108 例ASO 患者，根据其术后一年是否发生ISR，分为发生ISR 组（n=42）和未发生ISR 组（n=66）。实时荧光定量聚合酶链式反应（qRT-PCR）检测患者血清中miR-150 和miR-155 的相对表达水平；采用Pearson 相关性分析血清miR-150，miR-155 水平与低密度脂蛋白- 胆固醇（LDL-C）、超敏C 反应蛋白（hs-CRP）的相关性；采用多因素Logistic 回归分析影响ASO 患者发生ISR 的因素；采用受试者工作特征（ROC）曲线分析血清miR-150，miR-155 水平对ASO 患者发生ISR 的预测价值。结果 与未发生ISR 组相比，发生ISR 组血清miR-150（0.65±0.16 vs 1.01±0.09）水平显著降低，miR-155（1.68±0.39vs 1.02±0.11），LDL-C（2.85±0.76 mmol/L vs 2.21±0.62mmol/L），hs-CRP（2.36±0.47 mg/L vs 1.82±0.36mg/L）水平显著升高，差异具有统计学意义（t=4.785 ～ 14.957，均P ＜ 0.05）。经Pearson 相关性分析显示，血清miR-150 与LDL-C，hs-CRP 水平呈显著负相关（r=-0.465，-0.532，均P ＜ 0.05），血清miR-155 与LDL-C，hs-CRP 水平呈显著正相关（r=0.453，0.481，均P ＜ 0.05）。多因素Logistic 回归分析显示，血清miR-150 是影响ASO 患者发生ISR 的保护因素，血清miR-155，LDL-C 和hs-CRP 为影响ASO 患者发生ISR 的危险因素（均P ＜ 0.05）。ROC 曲线分析显示，血清miR-150，miR-155 及二者联合预测ASO 患者发生ISR 的AUC（95%CI）分别为0.869（0.791 ～ 0.926），0.874（0.796 ～ 0.930）及0.976（0.926 ～ 0.996），联合检测显著优于二者单独预测（Z =2.679，2.745，均P ＜ 0.05）。结论 ASO支架植入术后发生ISR 的患者血清miR-150 水平降低，miR-155 水平升高，均是影响ASO 患者发生ISR 的因素，且二者联合具有较高的预测价值。
Abstract:
Objective To explore the predictive value of serum microRNA (miR) -150 and microRNA (miR) -155 expression levels in patients with lower limb arteriosclerosis obliterans (ASO) for in-stent restenosis (ISR) after stent implantation. Methods A total of 108 ASO patients who received stent implantation in Deyang People’s Hospital form March 2020 to June 2022 were gathered. They were grouped into two groups based on whether they had experienced ISR one year after surgery: the ISR group (n=42) and the non-ISR group (n=66). Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was applied to detect the relative expression levels of miR-150 and miR-155 in serum. Pearson correlation was applied to analyze the correlation between serum miR-150, miR-155 levels and low-density lipoprotein cholesterol (LDL-C) and hypersensitive C-reactive protein (hs-CRP). Multivariate logistic regression was applied to analyze the factors influencing the occurrence of ISR in ASO patients. The receiver operating characteristic (ROC) curve was applied to analyze the predictive value of serum miR-150 and miR-155 levels for ISR in ASO patients. Results Compared with non-ISR group, the serum miR-150（0.65±0.16 vs 1.01±0.09）level in ISR group was greatly reduced, while the levels of miR-155（1.68±0.39 vs 1.02±0.11），LDL-C（2.85±0.76 mmol/L vs 2.21±0.62mmol/L）and hs-CRP（2.36±0.47 mg/L vs 1.82±0.36mg/L）were greatly increased ，and the differences were statistically significant (t=4.785 ～ 14.957，all P<0.05). According to Pearson correlation analysis, serum miR-150 was greatly negatively correlated with LDL-C and hs-CRP levels (r=-0.465, -0.532, all P<0.05)，the serum miR-155 level was greatly positively correlated with LDL-C and hs-CRP levels(r=0.453，0.481，all P<0.05). Multivariate Logistic regression analysis showed that serum miR-150 was a protective factor affecting the occurrence of ISR in ASO patients, while serum miR-155， LDL-C and hs-CRP were risk factors affecting the occurrence of ISR in ASO patients (all P<0.05). ROC curve showed that the AUC（95%CI）of serum miR-150, miR-155, and their combined prediction for ISR in ASO patients was 0.869（0.791 ～ 0.926）， 0.874（0.796 ～ 0.930）and 0.976（0.926 ～ 0.996），respectively, the combined detection was greatly better than the two individual predictions (Z=2.679，2.745, all P<0.05). Conclusion The miR-150 level decreased and the miR-155 level increased in patients with ISR after ASO stent implantation are both factors affecting the occurrence of ISR in ASO patients, and the combination detection of the two has high predictive value.

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备注/Memo

备注/Memo:
作者简介：张忠俊(1986-)，男，本科，主管技师，研究方向：影像技术，E-mail：312639964@qq.com。
通讯作者：陈琳（1988-），女，本科，主管技师，研究方向：影像技术，E-mail：358006573@qq.com。
更新日期/Last Update: 2025-03-15