[1]胡鹤鸣a,朱现菊a,钟艳艳,等.系统性红斑狼疮肾炎患者血清LRG1和Thiol水平表达及临床价值研究[J].现代检验医学杂志,2025,40(02):114-118.[doi:10.3969/j.issn.1671-7414.2025.02.021]
　HU Heminga,ZHU Xianjua,ZHONG Yanyan,et al.Study on the Expression and Clinical Value of Serum LRG1 and Thiol Levels in Patients with Systemic Lupus Erythematosus Nephritis[J].Journal of Modern Laboratory Medicine,2025,40(02):114-118.[doi:10.3969/j.issn.1671-7414.2025.02.021]
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系统性红斑狼疮肾炎患者血清LRG1和Thiol水平表达及临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
114-118
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Study on the Expression and Clinical Value of Serum LRG1 and Thiol Levels in Patients with Systemic Lupus Erythematosus Nephritis
文章编号:
1671-7414（2025）02-0114-05
作者:
胡鹤鸣1a; 朱现菊1a; 钟艳艳2; 王 虹1b
（1. 青岛市第八人民医院a. 检验科，b. 肾内科；山东青岛 266041；2. 青岛大学附属医院风湿免疫科，山东青岛266700）
Author(s):
HU Heming1a; ZHU Xianju1a; ZHONG Yanyan2 ;  WANG Hong1b
(1a. Department of Clinical Laboratory； 1b.Department of Nephrology, Qingdao Eighth People’s Hospital，Shandong Qingdao 266041, China；2. Department of Rheumatology and Immunology，Affiliated Hospital of Qingdao University，Qingdao Shandong 266700, China)
关键词:
系统性红斑狼疮肾炎; 富亮氨酸α-2 糖蛋白-1; 硫醇; 自身免疫抗体; 肾功能损伤
分类号:
R593.242；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.02.021
文献标志码:
A
摘要:
目的 探讨系统性红斑狼疮肾炎（SLEN）患者血清富亮氨酸α-2 糖蛋白-1（LRG1）、硫醇（Thiol）水平表达与自身免疫抗体及肾功能损伤的相关性。方法 选取2021 年1 月～ 2023 年12 月青岛市第八人民医院肾内科和青岛大学附属医院风湿免疫科收治的SLEN 患者80 例（SLEN 组）和单纯SLE 患者80 例（非SLEN 组）。酶联免疫吸附法（ELISA）检测血清LRG1 水平，比色法检测血清Thiols 水平。采用Pearson/Spearman 相关性分析SLEN 患者血清LRG1，Thiol与自身免疫抗体及肾功能指标的相关性；受试者工作特征（ROC）曲线分析血清LRG1，Thiol 水平对SLEN 的诊断价值。结果 SLEN 组血清LRG1（26.00±8.76μg/ml），Scr（98.03±49.71μmol/L）高于非SLEN 组(16.71±5.51μg/ml,73.85±33.51μmol/L)，Thiol（80.69±36.90 μmol/L）低于非SLEN 组(131.49±45.34 μmol/L)，差异具有统计学意义（t=8.026，3.608，7.772，均P ＜ 0.001）。Pearson/Spearman 相关性分析SLEN 患者血清LRG1 与抗双链-DNA（dsDNA）抗体阳性、抗补体1q（C1q）抗体阳性、抗Smith 抗体阳性、血肌酐（Scr）、24h 尿蛋白呈正相关（r=0.226 ～ 0.637，均P ＜ 0.05），与估计的肾小球滤过率（eGFR）呈负相关（r =-0.633，P ＜ 0.05）；血清Thiol 与抗dsDNA 抗体阳性、抗C1q 抗体阳性、抗Smith 抗体阳性、Scr，24h 尿蛋白呈负相关（r =-0.612 ～ -0.256，均P ＜ 0.05），与eGFR 呈正相关（r=0.614，P ＜ 0.05）。血清LRG1 联合Thiol 诊断SLEN 的AUC(95%CI) 为0.907（0.851 ～ 0.947），大于血清LRG1，Thiol 单独诊断的0.802（0.731 ～ 0.860），0.808（0.738 ～ 0.866），差异具有统计学意义（Z=3.450，3.749，均P ＜ 0.05）。结论 SLEN 患者血清LRG1 水平升高、Thiol 水平降低与自身免疫抗体阳性表达及肾功能损伤密切相关，联合检测血清LRG1 和Thiols 可为诊断SLEN 提供参考。
Abstract:
Objective To investigate the correlation of serum leucine-rich alpha-2 glycoprotein-1（LRG1） and Thiol levels with autoimmune antibodies and renal injury in patients with systemic lupus erythematosus nephritis (SLEN). Methods 80 SLEN patients (SLEN group) and 80 simple SLE patients (non-SLEN group) admitted to the Department of Nephrology at Qingdao Eighth People’s Hospital and the Department of Rheumatology and Immunology at Affiliated Hospital of Qingdao University from January 2021 to December 2023 were selected. An enzyme-linked immunosorbent assay (ELISA) was used to detect serum LRG1 levels, while a colorimetric assay was used to detect serum Thiol levels. Pearson/Spearman correlation analysis was used to investigate the correlation between serum LRG1 and Thiol levels, autoimmune antibodies, and renal function indicators in SLEN patients. The diagnostic value of serum LRG1 and Thiol levels in SLEN was analyzed using receiver characteristic curves. Results The serum LRG1 (26.00 ± 8.76 μg/ml) and Scr (98.03 ± 49.71μmol/L) in the SLEN group were higher than those in the non-SLEN group（16.71±5.51μg/ml，73.85±33.51μmol/L）, and Thiol (80.69 ± 36.90μmol/L) was lower than those in the non-SLEN group（131.49±45.34μmol/L）, the differences were statistically significant(t=8.026，3.608，7.772，all P<0.001). Pearson/Spearman correlation analysis showed that serum LRG1 in SLEN patients was positively correlated with antidouble- stranded deoxyribonucleic acid (dsDNA) antibody, anti-complement 1q (C1q) antibody, anti-Smith antibody, serum creatinine (Scr), 24-hour urine protein(r=0.226 ～ 0.637, all P<0.05), and negatively correlated with estimated glomerular filtration rate (eGFR) (r =-0.633, P<0.05). Serum Thiol was negatively correlated with anti-dsDNA antibodies, anti C1q antibodies, anti-Smith antibodies, Scr and 24-hour urine protein(r=-0.612 ～ -0.256, all P<0.05), and positively correlated with eGFR (r=0.614, P<0.05). The AUC (95% CI) of serum LRG1 combined with Thiol levels for diagnosing of SLEN was 0.907 (0.851 ～ 0.947), which was higher than that of serum LRG1 and Thiol alone at 0.802 (0.731 ～ 0.860) and 0.808 (0.738 ～ 0.866), and the differences were statistically significant (Z=3.450, 3.749, all P<0.05). Conclusion Elevated serum LRG1 levels and decreased Thiol levels in SLEN patients were closely related to positive expression of autoimmune antibodies and renal dysfunction. Combined detection of serum LRG1 and Thiols can provide reference for the diagnosis of SLEN.

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相似文献/References:

[1]王 燕a,胡秀娟a,张 丽a,等.溃疡性结肠炎患者血清Elabela，LRG1水平表达与疾病活动指数的相关性研究[J].现代检验医学杂志,2024,39(01):100.[doi:10.3969/j.issn.1671-7414.2024.01.018]
　WANG Yana,HU Xiujuana,ZHANG Lia,et al.Correlation between the Expression of Serum Elabela, LRG1 Levels and Disease Activity Index in Patients with Ulcerative Colitis[J].Journal of Modern Laboratory Medicine,2024,39(02):100.[doi:10.3969/j.issn.1671-7414.2024.01.018]

备注/Memo

备注/Memo:
基金项目：山东省医药卫生科技发展计划项目（202014050420）。
作者简介：胡鹤鸣(1978-)，女，硕士研究生，副主任技师，研究方向：检验学，E-mail：chenxiuying1258@163.com。
通讯作者：王虹（1968-），女，大学本科，主任医师，研究方向 ：肾内科，E-mail：15806565166@163.com。
更新日期/Last Update: 2025-03-15