[1]景本才,张 丽,周云飞.急性胰腺炎患者外周血CD4+T细胞内DsbA-L表达水平与病情严重程度和预后的关系研究[J].现代检验医学杂志,2025,40(03):102-107.[doi:10.3969/j.issn.1671-7414.2025.03.019]
　JING Bencai,ZHANG Li,ZHOU Yunfei.Study on the Relationship between the Expression Level of DsbA-L in Peripheral Blood CD4+ T Cells and the Severity and Prognosis of the Disease in Patients with Acute Pancreatitis[J].Journal of Modern Laboratory Medicine,2025,40(03):102-107.[doi:10.3969/j.issn.1671-7414.2025.03.019]
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急性胰腺炎患者外周血CD4+T细胞内DsbA-L表达水平与病情严重程度和预后的关系研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年03期
页码:
102-107
栏目:
论著
出版日期:
2025-05-15

文章信息/Info

Title:
Study on the Relationship between the Expression Level of DsbA-L in Peripheral Blood CD4+ T Cells and the Severity and Prognosis of the Disease in Patients with Acute Pancreatitis
文章编号:
1671-7414（2025）03-102-06
作者:
景本才1，张 丽2，周云飞1
（1．富顺县人民医院急诊科，四川富顺 643200；2．西南医科大学附属自贡医院/自贡市精神卫生中心老年科二病区，四川自贡 643000）
Author(s):
JING Bencai1，ZHANG Li2，ZHOU Yunfei1
（1．Department of Emergency Medicine，Fushun County People’s Hospital，Sichuan Fushun 643200，China；2．Geriatric Ward，Zigong Mental Health Center/Zigong Hospital Affiliated to Southwest Medical University，Sichuan Zigong 643000，China）
关键词:
急性胰腺炎; 二硫键A氧化还原酶样蛋白; 病情严重程度; CD4+T细胞
分类号:
R576；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.03.019
文献标志码:
A
摘要:
目的探究急性胰腺炎（AP）患者外周血CD4+T细胞内二硫键A氧化还原酶样蛋白（DsbA-L）表达水平与患者病情严重程度和预后的关系。方法选取2022年1月～2023年12月在富顺县人民医院就诊的172例AP患者为研究组，根据AP患者病情严重程度进一步分为轻症AP组（n=64）、中重症AP组（n=48）和重症AP组（n=60）；选取60例慢性胰腺炎（CP）患者为CP组；选取100例同期体检的健康者为对照组。实时荧光定量PCR（qRT-PCR）检测各组外周血CD4+T内DsbA-LmRNA表达水平；Pearson法分析DsbA-L水平与AP患者病情严重程度的相关性；多因素Logistic回归分析AP患者预后的影响因素。结果轻症AP组、中重症AP组和重症AP组的CRP，BUN，Scr，PCT，APACHEⅡ评分、BISAP评分依次升高，差异具有统计学意义（F=3.298～323.587，均P<0.05）。研究组DsbA-LmRNA表达水平（0.82±0.16）较对照组（1.08±0.21）和CP组（1.05±0.20）降低，差异具有统计学意义（t=11.254，8.350，均P<0.001）。中重症AP组（0.83±0.17）和重症AP组（0.73±0.15）DsbA-LmRNA表达水平较轻症AP组（0.92±0.20）降低（t=2.686，6.024），且重症AP组DsbA-LmRNA表达水平较中重症AP组降低（t=3.244）,差异具有统计学意义（均P<0.05）。Pearson法相关性分析，DsbA-L表达水平与CRP，BUN，PCT，APACHEⅡ评分、BISAP评分呈负相关（r=-0.693～-0.591，均P<0.001）。预后不良组DsbA-LmRNA（0.76±0.19）表达水平较预后良好组（0.98±0.23）降低，差异具有统计学意义（t=6.399,P<0.001）。多因素Logistic回归分析，DsbA-L水平降低为AP患者预后不良的独立危险因素(Waldχ2=6.757，P<0.05)。结论AP患者外周血CD4+T内DsbA-L表达水平降低与病情严重程度及预后密切相关，可作为评估AP患者病情严重程度及预后的标志物。
Abstract:
Objective To investigate the relationship between the expression level of disulfide bond A oxidoreductase-like protein (DsbA-L) in peripheral blood CD4+ T cells of patients with acute pancreatitis (AP) and the severity of disease and prognosis of patients. Methods 172 cases of AP patients attending the Fushun County People’s Hospital from January 2022 to December 2023 were selected as the study group. According to the degree of severity of the disease, AP patients were divided into mild AP group （n=64）, moderately severe AP group（n=48） and severe AP group（n=60）. 60 patients with chronic pancreatitis (CP) were selected as the CP group. 100 cases of healthy people who had physical examinations in the same period were selected as the control group. Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of DsbA-L mRNA in peripheral blood CD4+ T in each group; Pearson’s method was used to analyze the correlation between the level of DsbA-L and the severity of the disease of the patients with AP. Multifactorial Logistic regression analysis of factors influencing the prognosis of AP patients. Results CRP, BUN, Scr, PCT, APACHE II score and BISAP score increased sequentially，and the difference was statistically significant (F=3.298～323.587，all P<0.05). The study group (0.82±0.16) DsbA-L mRNA expression level was lower than that in the control group (1.08±0.21) and CP group (1.05±0.20), and the differences were statistically significant (t=11.254,8.350，all P<0.001) . DsbA-L mRNA in the moderately severe AP group (0.83±0.17) and severe AP group (0.73±0.15)expression levels were lower than those in the mild AP group (0.92±0.20),the expression level of DsbA-L mRNA in the severe AP group was significantly lower than that in the moderate to severe AP group and the differences were statistically significant (t=2.686, 6.024, 3.244, all P<0.05). Pearson correlation analysis , DsbA-L levels were negatively correlated with CRP, BUN, PCT, APACHE II score and BISAP score (r=-0.693～-0.591，all P<0.001) . DsbA-L mRNA expression level was lower in the poor prognosis group compared with the good prognosis group (0.98±0.23 vs 0.76±0.19), and the difference was statistically significant (t=6.399, P<0.001). Multivariate Logistic regression analysis, the reduced level of DsbA-L was an independent risk factor for poor prognosis in AP patients (Waldχ2=6.757，P<0.05). Conclusion Reduced levels of DsbA-L expression within CD4+ T in peripheral blood of AP patients are closely correlated with disease severity and prognosis, and can be used as a marker to assess disease severity and prognosis of AP patients.

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相似文献/References:

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备注/Memo

备注/Memo:
作者简介：景本才（1989-），男，本科，主治医师，研究方向：急症内科，急腹症、脓毒症救治，E-mail：dfge349579859@163.com。
通讯作者：周云飞（1989-），男，本科，主治医师，研究方向：急诊医学，E-mail：978634801@qq.com。
更新日期/Last Update: 2025-05-15