[1]吴 江,郭海丽.ATP2B2基因rs35678，rs2289274位点多态性与良性阵发性位置性眩晕的相关性研究[J].现代检验医学杂志,2025,40(04):13-17.[doi:10.3969/j.issn.1671-7414.2025.04.003]
　WU Jiang,GUO Haili.Correlation between Polymorphism at rs35678, rs2289274 Loci of ATP2B2 Gene and Benign Paroxysmal Positional Vertigo[J].Journal of Modern Laboratory Medicine,2025,40(04):13-17.[doi:10.3969/j.issn.1671-7414.2025.04.003]
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ATP2B2基因rs35678，rs2289274位点多态性与良性阵发性位置性眩晕的相关性研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年04期
页码:
13-17
栏目:
论著
出版日期:
2025-07-15

文章信息/Info

Title:
Correlation between Polymorphism at rs35678, rs2289274 Loci of ATP2B2 Gene and Benign Paroxysmal Positional Vertigo
文章编号:
1671-7414（2025）04-013-05
作者:
吴 江1,2，郭海丽2
（1. 西安工会医院耳鼻喉科，西安 710199；2. 陕西中医药大学第二附属医院耳鼻咽喉科，陕西咸阳 712000）
Author(s):
WU Jiang1,2，GUO Haili2
（1. Department of Otolaryngology，Xi’an Trade Union Hospital，Xi’an 710199，China； 2. Department of Otorhinolaryngology，the Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine，Shaanxi Xianyang 712000，China）
关键词:
良性阵发性位置性眩晕; 单核苷酸多态性; 钙离子转运ATP 酶B2
分类号:
R764.3；R786
DOI:
10.3969/j.issn.1671-7414.2025.04.003
文献标志码:
A
摘要:
目的 探究钙离子转运ATP 酶B2(ATP2B2) 基因rs35678，rs2289274 位点多态性与良性阵发性位置性眩晕(BPPV)的相关性。方法 选取2022 年1 月～ 2024 年1 月西安工会医院收治的BPPV 患者186 例作为BPPV 组，另选取同期体检的100 例健康者作为对照组。通过聚合酶链式反应(PCR) 检测ATP2B2 基因rs35678，rs2289274 位点的多态性；比较两组等位基因及基因型频率；非条件Logistic 回归法分析rs35678，rs2289274 位点三种遗传模型(共显性、显性和隐性)下与BPPV 易感性的相关性。结果 ATP2B2 基因rs35678，rs2289274 位点的基因型在对照组、BPPV 组的分布均符合Hardy-Weinberg 平衡定律(χ2=0.003 ～ 0.050，均P>0.05)，具有群体代表性。与对照组相比，BPPV 组rs35678 位点等位基因T 频率(56.45%vs 41.00%) 和基因型TT 频率(31.87% vs 16.81%) 升高，rs2289274 位点等位基因A 频率(54.30% vs42.00%) 和基因型AA 频率(29.48% vs 17.64%) 升高，差异具有统计学意义(χ2=3.936 ～ 12.290，均P<0.05)。rs35678 位点在共显性模型(CC vs TT) 下，TT 基因型携带者患病风险高于CC 基因型携带者(OR=1.851，95%CI：1.059 ～ 2.936)；显性模型(CT+TT vs CC) 和隐性模型(CT+CC vs TT) 下，rs35678 位点多态性与BPPV 的关联性(OR=1.716，95%CI：1.074~2.936；OR=0.759，95%CI：0.517 ～ 0.837)，差异具有统计学意义(均P<0.05)。rs2289274 位点在共显性模型(GGvs AA) 下，AA 基因型携带者患病风险高于GG 基因型携带者(OR=1.627，95%CI：1.191 ～ 2.973)；显性模型(AG+AAvs GG) 下，rs2289274 位点多态性与BPPV 的关联性(OR=1.941，95%CI：1.191 ～ 3.673)，差异具有统计学意义(均P<0.05)。结论 ATP2B2 基因rs35678 位点TT 基因型及rs2289274 位点AA 基因型会增加BPPV 患病风险。
Abstract:
Objective To investigate the association between polymorphisms at the rs35678, rs2289274 loci of the ATPase plasma membrane Ca2+ transporting 2 (ATP2B2) gene and benign paroxysmal positional vertigo (BPPV). Methods 186 BPPV patients admitted to Xi’an Trade Union Hospital from January 2022 to January 2024 were selected as the BPPV group, and another 100 healthy individuals who underwent physical examination during the same period were selected as the control group. Polymerase chain reaction (PCR) was used to detect polymorphisms at the rs35678 and rs2289274 loci of the ATP2B2 gene． Allele and genotype frequencies were compared between the two groups. Unconditional Logistic regression was used to analyze the correlation with BPPV susceptibility under three genetic models (co-dominant，dominant and recessive) for the rs35678 and the rs2289274 loci. Results The distribution of genotypes at the rs35678 and rs2289274 loci of the ATP2B2 gene in the control group, and the BPPV group were all in accordance with the Hardy-Weinberg law of equilibrium (χ2=0.003 ～ 0.050，all P>0.05), and had a representativeness of the groups．Compared with the control group, the allele T frequency(56.45%vs 41.00%) and genotype TT frequency (31.87% vs 16.81%) were significantly higher in the BPPV group at the rs35678 locus, and the allele A frequency(54.30% vs 42.00%) and genotype AA frequency (29.48% vs 17.64%) at the rs2289274 locus, and the differences were statistically significant (χ2=3.936 ～ 12.290，all P<0.05). Under the codominant model (CC vs TT) for the rs35678 locus, carriers of TT genotype had an increased risk of disease compared to carriers of CC genotype (OR=1.851，95% CI：1.059 ～ 2.936);Under both the dominant model (CT+TT vs CC) and recessive model (CT+CC vs TT), the rs35678 polymorphism showed a statistically significant association with BPPV (OR=1.716, 95%CI: 1.074~2.936; OR=0.759, 95%CI: 0.517~0.837), and the differences were statistically significant (all P<0.05), reqectively. Under the codominant model (GG vs AA) for the rs2289274 locus, carriers of the AA genotype had a higher risk of disease compared to carriers of the GG genotype (OR=1.627，95%CI： 1.191 ～ 2.973); in the dontinant model (AG+AA vs GG) the polymorphisms at the rs2289274 locus showed a significant association with BPPV (OR=1.941，95%CI：1.191 ～ 3.673), the differences were statistically significant (P<0.05). Conclusion The TT genotype at the rs35678 locus and the AA genotype at the rs2289274 locus of the ATP2B2 gene increase the risk of developing BPPV.

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备注/Memo

备注/Memo:
基金项目：陕西省科技厅重点研发计划项目- 社会发展领域 (项目编号:2022SF-563)。
作者简介：吴江（1980-），男，本科，主治医师，研究方向：耳鼻咽喉专业，E-mail：18192020576@163.com。
通讯作者：郭海丽（1987-），女，硕士研究生，主治医师，研究方向：耳鼻咽喉科学眩晕方向， E-mail：m13468651982@163.com。
更新日期/Last Update: 2025-07-15