[1]董 佳,董 莉,张保平,等.2017～2023年呼和浩特市HIV感染人群临床实验室化学发光法筛查结果与确证病例分析[J].现代检验医学杂志,2025,40(05):162-166.[doi:10.3969/j.issn.1671-7414.2025.05.031]
　DONG Jia,DONG Li,ZHANG Baoping,et al.Analysis of Screening Results and Confirmed Cases by Chemiluminescence Method in Clinical Laboratory of HIV Infected Population in Hohhot City from 2017 to 2023[J].Journal of Modern Laboratory Medicine,2025,40(05):162-166.[doi:10.3969/j.issn.1671-7414.2025.05.031]
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2017～2023年呼和浩特市HIV感染人群临床实验室化学发光法筛查结果与确证病例分析()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年05期
页码:
162-166
栏目:
论著
出版日期:
2025-09-15

文章信息/Info

Title:
Analysis of Screening Results and Confirmed Cases by Chemiluminescence Method in Clinical Laboratory of HIV Infected Population in Hohhot City from 2017 to 2023
文章编号:
1671-7414（2025）05-162-05
作者:
董 佳1,2，董 莉1,2，张保平1,2，赫晓霞2
1.内蒙古医科大学附属医院检验科，呼和浩特 010050;2.内蒙古医科大学附属医院检验
Author(s):
DONG Jia，DONG Li，ZHANG Baoping，HE Xiaoxia
Department of Clinical Laboratory, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China
关键词:
化学发光免疫分析; 人类免疫缺陷病毒; 免疫印迹法; 艾滋病
分类号:
R373；Q503
DOI:
10.3969/j.issn.1671-7414.2025.05.031
文献标志码:
A
摘要:
目的探讨人类免疫缺陷病毒（HIV）的感染现状、分布特点及化学发光免疫分析（CLIA）筛查HIV呈反应性样本S/CO值与确证试验阳性的关系。方法选择2017～2023年内蒙古医科大学附属医院检验科用雅培CLIA检出的820例HIV呈反应性结果的病例为研究对象，按不同年份、性别及年龄，用SPSS22.0软件进行统计学分析，比较筛查呈反应性和确证阳性的分布特点，回顾性分析确证试验（免疫印迹法）结果阳性、不确定和阴性与其S/CO值的关系，以及相应条带的分布情况。结果2017～2023年HIV确证试验阳性的年度总人数呈现先降后增趋势。确证阳性率为31.95%（262/820），男女比例为6.7∶1，性别差异具有统计学意义（χ2=126.8，P＜0.05）。筛查试验呈反应性的男女比例为1.4∶1。男性和女性确证阳性人数最多的年龄段均为30～50岁。根据S/CO值分为五组，1.0≤S/CO≤5.0组确证阳性率为0%（0/436），5.0＜S/CO≤10.0组确证阳性率为6.90%（4/58），10.0＜S/CO≤50.0组确证阳性率为25.86%（15/58），50.0＜S/CO≤500.0组确证阳性率为82.09%（110/134），S/CO＞500.0组确证阳性率为99.25%（133/134），五组间确证阳性人数差异具有统计学意义（χ2=816.3，P＜0.05）。确证阴性473例，女性多于男性，差异有统计学意义（χ2=98.3，P＜0.05），其中S/CO值≤10.0的结果占90.49%（428/473）；不确定85例，性别差异无统计学意义（χ2=1.3，P＞0.05），其中S/CO值≤10.0的结果占72.94%（62/85）。确证试验阳性中条带gp120和gp160在所有结果中均出现，p55出现次数最少，占51.91%（136/262）；不确定结果的条带以p24为主，其次为gp160等。在一定程度上，CLIA筛查试验S/CO值越高，确证试验为阳性时条带越多，确证试验为不确定时显现的条带也越多。结论CLIA筛查HIV时，S/CO值越高，确证试验阳性率越高，阳性条带也越多，对实验室分析检测结果和临床早期诊断具有实用价值。
Abstract:
Objective To investigate the status and distribution characteristics of human immuno-deficiency virus (HIV) infection, and the relationship between sample value/cut-off value (S/CO value) and positive test when HIV is tested reactivity by chemiluminescence immunoassay(CLIA). Methods The data of 820 HIV reactivity samples screened by Abbott CLIA in the Department of Clinical Laboratory of Affiliated Hospital of Inner Mongolia Medical University from 2017 to 2023 were selected as the research objects. SPSS22.0 software was used for statistical analysis according to different years, genders and ages. The distribution characteristics of screening reactivity and confirmed positive immunoblot test was compared. The relationship between the confirmed positive, uncertain and negative results and their S/CO values, and the distribution of corresponding bands were retrospectively analyzed. Results From 2017 to 2023, the total number of HIV positive patients decreased first decreased and then increased. The positive rate was 31.95% (262/820), the ratio of males to females was 6.7∶1, the gender difference was statistically significant (χ2 =126.8, P<0.05). The ratio of men to women who responded to the screening test was 1.4∶1. The age group with the most confirmed positives for both men and women was 30~50 years old. Divided into five groups according to S/CO value, the positive rate was 0% (0/436) in 1.0≤S/CO≤5.0 group, the positive rate was 6.90% (4/58) in 5.0＜S/CO≤10.0 group, the positive rate was 25.86% (15/58) in 10.0＜S/CO≤50.0 group, the positive rate was 82.09% (110/134) in 50.0＜S/CO≤500.0 group, and the positive rate was 99.25% (133/134) in S/CO＞500.0 group, there was statistical significance in the number of confirmed positives among the five groups (χ2=816.3, P<0.05).There were 473 confirmed negative cases, more women than men, the difference was statistically significant (χ2=98.3, P<0.05), among which the results of S/CO value≤10.0 accounted for 90.49% (428/473). There were 85 uncertain cases, the gender difference was not statistically significant (χ2=1.3, P>0.05), among which the results of S/CO value≤10.0 accounted for 72.94% (62/85). The positive bands gp120 and gp160 appeared in all the results, and p55 appeared the least, accounted for 51.91% (136/262). The uncertain bands were mainly p24, followed by gp160, etc. To a certain extent, the higher the S/CO value of chemiluminescence screening test, the more bands were present when the confirmative test was positive, and the more bands were present when the confirmative test was uncertain. Conclusion The higher the S/CO value of chemiluminescence test for HIV screening test, the higher the positive rate of the confirmation test and the more positive bands, they have practical value for laboratory analysis and early clinical diagnosis.

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备注/Memo

备注/Memo:
作者简介：董佳（1989-），女，本科，主管检验师，主要从事临床免疫检验研究。
通讯作者：董莉（1963-），女，主任检验师，主要从事临床免疫检验研究，E-mail：15047868156@163.com。
更新日期/Last Update: 2025-09-15