[1]王 敏,韩 悠,赵军波,等.糖尿病视网膜病变患者血清circHOMER1，miR-23a-3p水平表达与临床分期以及氧化应激的相关性[J].现代检验医学杂志,2025,40(06):104-109.[doi:10.3969/j.issn.1671-7414.2025.06.019]
　WANG Min,HAN You,ZHAO Junbo,et al.Correlation of Serum circHOMER1, miR-23a-3p Levels with Clinical Stages and Oxidative Stress in Patients with Diabetic Retinopathy[J].Journal of Modern Laboratory Medicine,2025,40(06):104-109.[doi:10.3969/j.issn.1671-7414.2025.06.019]
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糖尿病视网膜病变患者血清circHOMER1，miR-23a-3p水平表达与临床分期以及氧化应激的相关性()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年06期
页码:
104-109
栏目:
论著
出版日期:
2025-11-15

文章信息/Info

Title:
Correlation of Serum circHOMER1, miR-23a-3p Levels with Clinical Stages and Oxidative Stress in Patients with Diabetic Retinopathy
文章编号:
1671-7414（2025）06-104-06
作者:
王 敏1，韩 悠1，赵军波1，崔 翠1，李佳佳1，霍 楠1，李 杏2
1.邯郸市中心医院眼科，河北邯郸 056000;2.廊坊市中医医院眼科，河北廊坊 065000
Author(s):
WANG Min1，HAN You1，ZHAO Junbo1，CUI Cui1，LI Jiajia1，HUO Nan1，LI Xing2
1.Department of Ophthalmology, Handan Central Hospital, Hebei Handan 056000, China;2.Department of Ophthalmology, Langfang Hospital of Traditional Chinese Medicine, Hebei Langfang 065000, China
关键词:
糖尿病视网膜病变; 环状核糖核酸HOMER1; 微小核糖核酸23a-3p; 临床分期; 氧化应激
分类号:
R587.2；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.06.019
文献标志码:
A
摘要:
目的探究糖尿病视网膜病变（DR）患者血清环状核糖核酸HOMER1（circHOMER1）、微小核糖核酸（miR）-23a-3p水平与临床分期以及氧化应激的相关性。方法选取2023年1月～2024年7月邯郸市中心医院眼科诊治的75例DR患者为DR组，根据DR临床分期分为非增生型DR（NPDR组，n=43）和增生型DR（PDR组，n=32）。另选取同期就诊的75例单纯2型糖尿病患者为非DR组。检测血清circHOMER1，miR-23a-3p，丙二醛（MDA）、超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）水平，收集受试者临床资料，利用TargetScan网站预测circHOMER1，miR-23a-3p的靶向关系。采用Pearson分析血清circHOMER1，miR-23a-3p之间及其与MDA，SOD，GSH的相关性；采用单因素和多因素Logistic回归分析2型糖尿病患者进展为DR的影响因素；采用受试者工作特征（ROC）曲线分析血清circHOMER1，miR-23a-3p对2型糖尿病患者进展为DR的预测价值。结果circHOMER1与miR-23a-3p之间存在靶向关系。DR组血清MDA（28.66±4.52ng/ml），circHOMER1（1.24±0.16）高于非DR组（16.95±3.27ng/ml，1.02±0.11），SOD（45.39±7.84U/L），GSH（135.82±21.23μg/ml），miR-23a-3p（0.88±0.07）低于非DR组（81.65±11.47U/L，207.44±25.95μg/ml，1.01±0.09），差异具有统计学意义（t=9.813～22.602，均P<0.001）。PDR组血清MDA（33.28±4.96ng/ml），circHOMER1（1.36±0.20）高于NPDR组（25.23±3.58ng/ml，1.15±0.17），SOD（34.39±7.15U/L），GSH（113.50±20.17μg/ml），miR-23a-3p（0.79±0.07）低于NPDR组（53.27±8.44U/L，152.43±23.99μg/ml，0.94±0.08），差异具有统计学意义（t=4.906～10.376，均P<0.001）。Spearman法分析显示，血清MDA，circHOMER1与DR严重程度呈正相关（r=0.533，0.473，均P<0.001），SOD，GSH，miR-23a-3p与DR严重程度呈负相关（r=-0.552，-0.515，-0.529，均P<0.001）。Pearson法分析显示，血清circHOMER1与SOD，GSH，miR-23a-3p呈负相关（r=-0.475，-0.460，-0.455，均P<0.001），与MDA呈正相关（r=0.462，P<0.001）；血清miR-23a-3p与SOD，GSH呈正相关，与MDA呈负相关（r=0.428，0.437，-0.439，均P<0.001）。Logistic回归法分析显示，高MDA，低SOD，低GSH，高circHO-MER1，低miR-23a-3p，高FPG和高HbA1c是2型糖尿病患者进展为DR的危险因素（OR=0.214～3.556，均P<0.05）。ROC分析显示，血清circHOMER1，miR-23a-3p单独预测和联合预测2型糖尿病患者进展为DR的曲线下面积（AUC）分别为0.751，0.797，0.903，联合预测高于单独预测（Z=3.179，2.335，均P<0.05）。结论DR患者血清MDA，circHOMER1水平较高，血清SOD，GSH，miR-23a-3p水平较低，血清circHOMER1，miR-23a-3p表达异常与DR进展和氧化应激有关，联合检测血清circHOMER1，miR-23a-3p对2型糖尿病患者进展为DR具有预测价值。
Abstract:
Objective To explore the correlation between serum circularRNA-HOMER1 (circHOMER1), microRNA(miR)-23a-3p levels with clinical stages and oxidative stress in patients with diabetic retinopathy (DR). Methods From January 2023 to July 2024, 75 DR patients treated in Handan Central Hospital were included as the DR group. According to the clinical staging of DR, they were divided into non proliferative DR (NPDR group, n=43) and proliferative DR (PDR group, n=32). In addition, 75 patients with simple type 2 diabetes who came to Handan Central Hospital were included as non DR group. The levels of serum circHOMER1, miR-23a-3p, malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH) were detect-ed. Clinical data of the subjects were collected. The TargetScan website was used to predict the targeting relationship between circHOMER1 and miR-23a-3p. Pearson method was used to analyze the correlation between serum circHOMER1, miR-23a-3p and MDA, SOD, GSH. Univariate and multivariate Logistic regression were used to analyze the influencing factors of progression of DR in type 2 diabetes patients. Receiver operating characteristic (ROC) carve was used to analyze the predictive value of serum circHOMER1 and miR-23a-3p in the progression of DR in patients with type 2 diabetes. Results There was a targeted relationship between circHOMER1 and miR-23a-3p. The serum MDA (28.66±4.52ng/ml) and circHOMER1 (1.24±0.16) levels in the DR group were higher than those in the non DR group(16.95±3.27ng/ml，1.02±0.11), while SOD (45.39±7.84U/L), GSH (135.82±21.23μg/mL) and miR-23a-3p (0.88±0.07) levels were lower than those in the non DR group(81.65±11.47U/L, 207.44±25.95μg/mL, 1.01±0.09）, and differences were statistically significant (t=9.813 ~ 22.602, all P<0.001). The serum MDA (33.28±4.96ng/ml) and circHOMER1 (1.36±0.20) levels in the PDR group were higher than those in the NPDR group(25.23±3.58ng/ml, 1.15±0.17), while SOD (34.39±7.15U/L), GSH (113.50±20.17μg/ml) and miR-23a-3p (0.79±0.07) levels were lower than those in the NPDR group(53.27±8.44U/L, 152.43±23.99μg/ml, 0.94±0.08), and the differences were statistically significant (t=4.906 ~ 10.376, all P<0.001). Spearman analysis showed that serum MDA and circHOMER1 were positively correlated with the severity of DR (r=0.533, 0.473, all P<0.001), while SOD, GSH, miR-23a-3p were negatively correlated with the severity of DR (r=-0.552, -0.515, -0.529, all P<0.001).Pearson analysis showed that serum circHOMER1 was negatively correlated with miR-23a-3p, SOD, GSH, and positively correlated with MDA (r=-0.475, -0.460,-0.455, 0.462, all P<0.001). Serum miR-23a-3p was positively correlated with SOD and GSH, and negatively correlated with MDA (r=0.428, 0.437, -0.439, all P<0.001).Logistic regression analysis showed that high MDA, low SOD, low GSH, high circHOMER1, low miR-23a-3p, high FPG and high HbA1c were the risk factors of progression of DR in type 2 diabetes patients (OR=0.214 ~ 3.556, all P <0.05). The area under curve (AUC) of serum circHOMER1 and miR-23a-3p alone and jointhy predicting the progression of DR in type 2 diabetes patients were 0.751, 0.797 and 0.903 respectively. The combined prediction was higher than that of serum circHOMER1 and miR-23a-3p alone (Z=3.179, 2.335, P=0.002, 0.020). Conclusion Serum MDA and circHOMER1 levels are higher in DR patients, while serum SOD, GSH and miR-23a-3p levels are lower. Abnormal expression of circHOMER1 and miR-23a-3p in serum is associated with progression of DR and oxidative stress. Combined detection of circHOMER1 and miR-23a-3p in serum can predict the progression of DR in patients with type 2 diabetes.

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备注/Memo

备注/Memo:
基金项目：邯郸市科学技术研究与发展计划项目（21422083115）。
作者简介：王敏（1973-），女，本科，副主任医师，研究方向：眼底病，E-mail：f30bcw@163.com
。
更新日期/Last Update: 2025-11-15