[1]王 峥,胡人芳,陈 哲,等.孕妇血清FA,MCP-1,PIBF水平检测与早期妊娠胚胎停止发育的相关性研究[J].现代检验医学杂志,2025,40(06):131-136.[doi:10.3969/j.issn.1671-7414.2025.06.024]
 WANG Zheng,HU Renfang,CHEN Zhe,et al.Study on the Correlation between Maternal Serum Levels of FA, MCP-1, PIBF and Embryo Stop Development in Early Pregnancy[J].Journal of Modern Laboratory Medicine,2025,40(06):131-136.[doi:10.3969/j.issn.1671-7414.2025.06.024]
点击复制

孕妇血清FA,MCP-1,PIBF水平检测与早期妊娠胚胎停止发育的相关性研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年06期
页码:
131-136
栏目:
论著
出版日期:
2025-11-15

文章信息/Info

Title:
Study on the Correlation between Maternal Serum Levels of FA, MCP-1, PIBF and Embryo Stop Development in Early Pregnancy
文章编号:
1671-7414(2025)06-131-06
作者:
王 峥胡人芳陈 哲张先华潘凤莲
荆州市第二人民医院妇产科,湖北荆州 434000
Author(s):
WANG ZhengHU RenfangCHEN ZheZHANG XianhuaPAN Fenglian
Department of Obstetrics and Gynecology, the Second Hospital of Jingzhou, Hubei Jingzhou 434000, China
关键词:
叶酸单核细胞趋化蛋白-1孕酮诱导的阻断因子早期妊娠胚胎停止发育
分类号:
R714.431;R392.11
DOI:
10.3969/j.issn.1671-7414.2025.06.024
文献标志码:
A
摘要:
目的研究孕妇血清叶酸(FA)、单核细胞趋化蛋白-1(MCP-1)和孕酮诱导的阻断因子(PIBF)水平与早期妊娠胚胎停止发育的相关性。方法选取2021年12月~2023年12月荆州市第二人民医院收治的早期妊娠胚胎停止发育孕妇98例为胎停组,另选同期进行孕检的正常早期妊娠孕妇50例为对照组,收集一般临床资料,酶联免疫吸附试验(ELISA)检测血清FA,MCP-1和PIBF水平。多因素Logistic回归分析早期妊娠胚胎停止发育的影响因素;绘制受试者工作特征(ROC)曲线分析血清FA,MCP-1和PIBF对早期妊娠胚胎停止发育的预测价值;Pearson法分析血清FA,MCP-1,PIBF与孕酮(PROG)、雌二醇(E2)以及β-人绒毛膜促性腺激素(β-HCG)的相关性。结果与对照组相比,胎停组的血清FA(9.51±1.21nmol/Lvs11.32±1.56nmol/L),PIBF(295.46±30.22ng/mlvs342.14±36.97ng/ml)水平降低,血清MCP-1(1.02±0.15mg/mlvs0.82±0.11mg/ml)水平升高,差异具有统计学意义(t=7.785,8.347,8.229,均P<0.001)。两组孕妇的胎停史(75.64%vs25.36%),PROG(13.32±1.81ng/mlvs23.65±2.74ng/ml),E2(221.34±25.69pmol/Lvs298.65±31.64pmol/L)以及β-HCG(5323.62±536.85U/Lvs8562.31±924.55U/L)水平比较,差异具有统计学意义(t/χ2=6.548~27.428,均P<0.05)。孕妇胎停史、MCP-1水平升高为影响早期妊娠胎停的危险因素(Waldχ2=4.239,4.613,均P<0.05),β-HCG,FA以及PIBF水平升高为影响早期妊娠胎停的保护因素(Waldχ2=4.476,4.423,5.974,均P<0.05);FA,MCP-1,PIBF以及联合预测早期妊娠胚胎停止发育的AUC分别为0.811,0.805,0.816和0.908,联合预测显著优于FA,MCP-1,PIBF单独预测(Z=2.749,2.381,1.993,均P<0.05)。FA,PIBF与PROG,E2,β-HCG呈正相关(r=0.433~0.512,均P<0.05),MCP-1与PROG,E2,β-HCG呈负相关(r=-0.432,-0.487,-0.458,均P<0.05)。结论早期妊娠胚胎停止发育孕妇血清FA,PIBF水平降低,MCP-1水平升高,三者均为影响孕妇胚胎停止发育的影响因素,对早期妊娠胚胎停止发育具有一定辅助预测价值。
Abstract:
Objective To investigate the correlation between maternal serum folic acid (FA), monocyte chemoattractant protein-1 (MCP-1), progesterone-induced blocking factor (PIBF) levels and embryonic development cessation in early pregnancy. Methods From December 2021 to December 2023, 98 pregnant women with embryonic development cessation in early pregnancy admitted to the Second Hospital of Jingzhou were regarded as the cessation group, and 50 normal early pregnancy pregnant women who underwent pregnancy examinations during the same period were as the control group. General clinical data was collected and analyzed. Enzyme-linked immunosorbent assay (ELISA) was applied to detect serum levels of FA, MCP-1 and PIBF. Multivariate logistic regression was applied to analyze the influencing factors of early pregnancy embryo cessation of development. Receiver operating characteristic (ROC) curve was plotted to analyze the predictive value of serum FA, MCP-1, and PIBF for early embryonic development cessation in pregnancy. Pearson method was applied to analyze the correlation between serum FA, MCP-1, PIBF, progesterone(PROG), estradiol(E2) and β-human chorionic gonadotropin (β-HCG). Results Compared with the control group, the serum FA (9.51±1.21 nmol/L vs 11.32±1.56 nmol/L) and PIBF (295.46±30.22 ng/ml vs 342.14±36.97 ng/ml) levels in the cessation group were greatly reduced, while the serum MCP-1 (1.02±0.15 mg/ml vs 0.82±0.11 mg/ml) level was greatly increased, and the differences were statistically significant (t=7.785, 8.347, 8.229, all P<0.001). There were great statistical differences in the history of embryonic development cessation (75.64% vs 25.36%), PROG(13.32±1.81 ng/ml vs 23.65±2.74 ng/ml ), E2 (221.34±25.69 pmol/L vs 298.65±31.64 pmol/L), and β-HCG levels(5 323.62±536.85U/L vs 8 562.31±924.55 U/L) between the two groups (t/χ2=6.548~27.428, all P<0.05). Pregnant women’s history of embryonic development cessation and elevated level of MCP-1 were risk factors for embryonic development cessation in early pregnancy (Wald χ2=4.239, 4.613, all P<0.05), while elevated levels of β-HCG, FA and PIBF were protective factors for embryonic development cessation in early pregnancy (Wald χ2=4.476, 4.423, 5.974, all P<0.05). The AUC of FA, MCP-1, PIBF, and their combination in predicting early embryonic development cessation in pregnancy was 0.811, 0.805, 0.816 and 0.908, respectively. The combined prediction was greatly better than that of individual diagnosis of FA MCP-1, and PIBF (Z=2.749, 2.381, 1.993, all P<0.05). FA and PIBF were positively correlated with PROG, E2 and β-HCG (r=0.433~0.512, all P<0.05), while MCP-1 was negatively correlated with PROG, E2 and β-HCG (r=-0.432, -0.487, -0.458, all P<0.05). Conclusion The serum levels of FA and PIBF in pregnant women with embryonic development cessation in early pregnancy decrease, while the level of MCP-1 increases. These three factors are all influencing factors for embryonic development cessation in pregnant women, and have certain auxiliary predictive value for embryonic development cessation in early pregnancy.

参考文献/References:

[1] XU Y, WANG R X, PANG Z, et al. Novel homozygous PADI6 variants in infertile females with early embryon-ic arrest[J]. Frontiers in Cell and Developmental Biolo-gy, 2022, 10: 819667.
[2] ZHANG Y L, ZHENG W, REN P P, et al. Biallelic mu-tations in MOS cause female infertility characterized by human early embryonic arrest and fragmentation[J]. EMBO Molecular Medicine, 2021, 13(12): e14887.
[3] 王栋,谭俊英,杨先礼.宫内妊娠胚胎停止发育的特征性超声表现[J].生命科学仪器,2023,21(S01):343. WANG D, TAN J Y, YANG X L. Characteristic ultrasound manifestations of embryonic arrest in intrauterine preg-nancy[J]. Life Science Instruments, 2023, 21(S01): 343.
[4] WANG L, CHEN J Y, HE L, et al. Association between the vaginal and uterine microbiota and the risk of early embryon-ic arrest[J]. Frontiers in Microbiology, 2023, 14: 1137869.
[5] LIU Y J, GENG T T, WAN Z Z, et al. Associations of serum folate and vitamin B12 levels with cardiovascu-lar disease mortality among patients with type 2 diabe-tes[J]. JAMA Network Open, 2022, 5(1): e2146124.
[6] LEE Y, PARK S. Serum folate levels and hyperten-sion[J]. Scientific Reports, 2022, 12(1): 10071.
[7] CHEN X T, ZHANG Y, CHEN H Y, et al. Association of maternal folate and vitamin B12 in early pregnancy with gestational diabetes mellitus:a prospective cohort study[J]. Diabetes Care, 2021, 44(1): 217-223.
[8] KANG J K, POSTIGO-FERNANDEZ J, KIM K J, et al. Notch-mediated hepatocyte MCP-1 secretion causes liver fibrosis[J]. JCI Insight, 2023, 8(3): e165369.
[9] LIU Y L, XU K, XIANG Y H, et al. Role of MCP-1 as an inflammatory biomarker in nephropathy[J]. Frontiers in Immunology, 2024, 14: 1303076.
[10] LIM M K, KU C W, TAN T C, et al. Characterisation of serum progesterone and progesterone-induced blocking factor (PIBF) levels across trimesters in healthy preg-nant women[J]. Scientific Reports, 2020, 10(1): 3840.
[11] CHECK J H, CHECK D L. A hypothetical model sug-gesting some possible ways that the progesterone receptor may be involved in cancer proliferation[J]. International Journal of Molecular Sciences, 2021, 22(22): 12351.
[12] LIANG Q, TONG L, XIANG L, et al. Correlations of the expression of γδ T cells and their co-stimulatory mole-cules TIGIT, PD-1, ICOS and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion[J]. Clinical and Experimental Immunology, 2021, 203(1): 55-65.
[13] 李军.妊娠早期孕妇胚胎停止发育的影响因素分析[J].现代诊断与治疗,2021,32(4):590-591. LI J. Analysis of factors influencing the cessation of embry-onic development in pregnant women in early pregnancy[J]. Modern Diagnosis and Treatment, 2021, 32(4): 590-591.
[14] PETERS S A E, YANG L, GUO Y, et al. Pregnancy, pregnancy loss and the risk of diabetes in Chinese wom-en: findings from the China Kadoorie Biobank[J]. Euro-pean Journal of Epidemiology, 2020, 35(3): 295-303.
[15] CAO Y, LIANG C M, SHEN L C, et al. The association between essential trace element (copper, zinc, selenium, and cobalt) status and the risk of early embryonic arrest among women undergoing assisted reproductive techniques[J]. Frontiers in Endocrinology(Lausanne), 2022, 13: 906849.
[16] BISWAS L, TYC K, EL YAKOUBI W, et al. Meiosis inter-rupted: the genetics of female infertility via meiotic failure[J]. Reproduction (Cambridge, England), 2021, 161(2): R13-R35.
[17] GU J Y, LEI C Y, ZHANG M X. Folate and retinal vascu-lar diseases[J]. BMC Ophthalmology, 2023, 23(1): 413.
[18] 王维,唐静,杨永红,等.妊娠期高血压疾病患者胎盘组织叶酸代谢基因MTR的表达水平及其作用机制研究[J].现代检验医学杂志,2022,37(4):134-138, 173. WANG W, TANG J, YANG Y H, et al. Expression level and effects of folate metabolism-related gene MTR in placenta of patients with hypertensive disorder com-plicating pregnancy[J]. Journal of Modern Laboratory Medicine, 2022, 37(4): 134-138, 173.
[19] WILLIAMSON J M, ARTHURS A L, SMITH M D, et al. High folate, perturbed one-carbon metabolism and gesta-tional diabetes mellitus[J]. Nutrients, 2022, 14(19): 3930.
[20] FU J B, LIU Y, WANG C, et al. Persistent follicular granulosa cell senescence and apoptosis induced by methotrexate leading to oocyte dysfunction and aber-rant embryo development[J]. Clinical and Translational Science, 2021, 14(5): 2043-2054.
[21] 王松伟,闫园园,孙涛,等.胎停育危险因素的meta分析[J].中国计划生育学杂志,2022,30(5):968-975. WANG S W, YAN Y Y, SUN T, et al. Meta-analysis of risk factors related to embryo damage[J]. Chinese Jour-nal of Family Planning, 2022, 30(5): 968-975.
[22] SINGH S, ANSHITA D, RAVICHANDIRAN V. MCP-1:function, regulation, and involvement in disease[J]. Inter-national Immunopharmacology, 2021, 101(Pt B): 107598.
[23] 陈婕,张红梅,谭春艳.脓毒症患者肠道菌群生态特征与血清D-乳酸、sTREM1和MCP-1表达水平的相关性研究[J].现代检验医学杂志,2023,38(4):139-142, 179. CHEN J, ZHANG H M, TAN C Y. Correlation between intestinal flora ecological characteristics and serum D-lactic acid,sTREM1 and MCP-1 expression levels in patients with sepsis[J]. Journal of Modern Laboratory Medicine, 2023, 38(4): 139-142, 179.
[24] HEIDARI S, KOLAHDOUZ-MOHAMMADI R, KHODAVERDI S, et al. Expression levels of MCP-1, HGF, and IGF-1 in endometriotic patients compared with non-endometriotic controls[J]. BMC Women’s Health, 2021, 21(1): 422.
[25] QUENBY S, GALLOS I D, DHILLON-SMITH R K, et al. Miscarriage matters: the epidemiological, physical, psy-chological, and economic costs of early pregnancy loss[J]. Lancet (London, England), 2021, 397(10285): 1658-1667.
[26] COTTRELL J N, WITCHER A C, COMLEY K, et al. Progesterone-induced blocking factor improves blood pressure, inflammation, and pup weight in response to reduced uterine perfusion pressure (RUPP)[J]. Ameri-can Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2021, 320(5): R719-R727.
[27] CSABAI T, PALLINGER E, KOVACS A F, et al. Altered immune response and implantation failure in progesterone-induced blocking factor-deficient mice[J]. Frontiers in Immunology, 2020, 11: 349.
[28] ADAMCZAK R, UKLEJA S N, LIS K, et al. Proges-terone-induced blocking factor 1 and cytokine profile of follicular fluid of infertile women qualified to in vitro fertilization: the influence on fetus development and pregnancy outcome[J]. International Journal of Immunopathology and Pharmacology, 2022, 36:3946320221111134.

相似文献/References:

[1]张瑞.脑梗死患者血清同型半胱氨酸、血浆叶酸和维生素B12水平的相关性研究[J].现代检验医学杂志,2015,30(05):118.[doi:10.3969/j.issn.1671-7414.2015.05.036]
 ZHANG Rui.Correlation Study on Homocysteine,Plasma Folate and Vitamin B12 Levels in Serum of Cerebral Infarction Patients[J].Journal of Modern Laboratory Medicine,2015,30(06):118.[doi:10.3969/j.issn.1671-7414.2015.05.036]
[2]孙建方,蔡新红,蒋冯霞,等.江苏省张家港地区妊娠妇女红细胞叶酸水平调查[J].现代检验医学杂志,2018,33(05):117.[doi:10.3969/j.issn.1671-7414.2018.05.032]
 SUN Jian-fang,CAI Xin-hong,JIANG Feng-xia,et al.Investigation of Erythrocyte Folic Acid Level in Pregnant Women in Zhangjiagang of Jiangsu[J].Journal of Modern Laboratory Medicine,2018,33(06):117.[doi:10.3969/j.issn.1671-7414.2018.05.032]
[3]王 艳,张 华,阮华玲,等.老年颈动脉粥样硬化叶酸、VitB12治疗患者血清HIF-1α,CD62p和hsCRP水平表达的变化[J].现代检验医学杂志,2018,33(06):99.[doi:10.3969/j.issn.1671-7414.2018.06.026]
 WANG Yan,ZHANG Hua,RUAN Hua-ling,et al.Changes of Serum HIF-1α,CD62p and hsCRP Expression in Elderly Patients Treated with Folic Acid and VitB12[J].Journal of Modern Laboratory Medicine,2018,33(06):99.[doi:10.3969/j.issn.1671-7414.2018.06.026]
[4]曹云,周晓,张晓艳,等.陕西省延安地区育龄女性MTHFR基因多态性和国内多地区相关报道比较分析2)[J].现代检验医学杂志,2019,34(01):62.[doi:10.3969/j.issn.1671-7414.2019.01.016]
 CAO Yun,ZHOU Xiao,ZHANG Xiao-yan,et al.Comparative Analysis of MTHFR Gene Polymorphisms in Women of Childbearing Age in Yan’an Area of Shaanxi Province and Related Reports in Many Regions in China[J].Journal of Modern Laboratory Medicine,2019,34(06):62.[doi:10.3969/j.issn.1671-7414.2019.01.016]
[5]段侠霞,陈英,沈德华,等.儿童及青少年精神分裂症患者血清同型半胱氨酸、叶酸水平与PANSS评分的关系研究[J].现代检验医学杂志,2019,34(01):104.[doi:10.3969/j.issn.1671-7414.2019.01.027]
 DUAN Xia-xia,CHEN Ying,SHEN De-hua,et al.Correlation Study between Serum Homocysteine,Folic Acid Levels and PANSS Score in Children and Adolescents Patients with Schizophrenia[J].Journal of Modern Laboratory Medicine,2019,34(06):104.[doi:10.3969/j.issn.1671-7414.2019.01.027]
[6]左 林,赵 佳,姜小建,等.血清同型半胱氨酸、叶酸、维生素 B12水平与老年脑白质疏松症的相关性[J].现代检验医学杂志,2019,34(06):90.[doi:10.3969 / j.issn.1671-7414.2019.06.022]
 ZUO Lin,ZHAO Jia,JIANG Xiao-jian,et al.Correlation Study between Serum Homocysteine , Folate, Vitamin B12 Levels and Leukoaraiosis in Elderly Patients[J].Journal of Modern Laboratory Medicine,2019,34(06):90.[doi:10.3969 / j.issn.1671-7414.2019.06.022]
[7]师文涛,白 蓉,黄剑林,等.陕西省延安地区汉族与国内其他地区育龄妇女 MTHFR 基因 C677T 位点基因型和等位基因分布研究[J].现代检验医学杂志,2020,35(06):78.[doi:doi:10.3969/j.issn.1671-7414.2020.06.019]
 SHI Wen-tao,BAI Rong,HUANG Jian-lin,et al.Comparative Study on MTHFR Gene C677T Locus Genotype and AlleleDistribution between Han Nationality and Women of ChildbearingAge in Yan’an Area of Shaanxi Province[J].Journal of Modern Laboratory Medicine,2020,35(06):78.[doi:doi:10.3969/j.issn.1671-7414.2020.06.019]
[8]毕开湘,陶红莉,杨丽丽.孤独症谱系障碍儿童血清叶酸与脑源性神经营养因子水平检测的临床意义[J].现代检验医学杂志,2020,35(06):171.[doi:doi:10.3969/j.issn.1671-7414.2020.06.042]
 BI Kai-xiang 1a,TAO Hong-li 2,YANG Li-li 1b.Clinical Significance of the Detection of Folic Acid and Brain-derivedNeurotrophic Factor in Children with Autism Spectrum Disorder[J].Journal of Modern Laboratory Medicine,2020,35(06):171.[doi:doi:10.3969/j.issn.1671-7414.2020.06.042]
[9]陈 婕,张红梅,谭春艳.脓毒症患者肠道菌群生态特征与血清D- 乳酸、sTREM1 和MCP-1 表达水平的相关性研究[J].现代检验医学杂志,2023,38(04):139.[doi:10.3969/j.issn.1671-7414.2023.04.025]
 CHEN Jie,ZHANG Hongmei,TAN Chunyan.Correlation between Intestinal Flora Ecological Characteristics and Serum D-lactic Acid,sTREM1 and MCP-1 Expression Levels in Patients with Sepsis[J].Journal of Modern Laboratory Medicine,2023,38(06):139.[doi:10.3969/j.issn.1671-7414.2023.04.025]

备注/Memo

备注/Memo:
基金项目:湖北省卫生健康科研立项项目(WJ2021M080)。
作者简介:王峥(1986-),女,本科,主治医师,研究方向:妇产科相关疾病研究,E-mail:hzgl2vj@163.com。
更新日期/Last Update: 2025-11-15