[1]秦晓林,段 纯,李俊祥,等.间接法建立深圳地区育龄期健康女性血清抗缪勒氏管激素参考区间及验证[J].现代检验医学杂志,2025,40(06):196-200.[doi:10.3969/j.issn.1671-7414.2025.06.036]
　QIN Xiaolin,DUAN Chun,LI Junxiang,et al.Application of Indirect Method in the Establishment Reference Interval for Anti-Miillerian Hormone in Healthy Women of Childbearing Age in Shenzhen Area[J].Journal of Modern Laboratory Medicine,2025,40(06):196-200.[doi:10.3969/j.issn.1671-7414.2025.06.036]
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间接法建立深圳地区育龄期健康女性血清抗缪勒氏管激素参考区间及验证()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年06期
页码:
196-200
栏目:
论著
出版日期:
2025-11-15

文章信息/Info

Title:
Application of Indirect Method in the Establishment Reference Interval for Anti-Miillerian Hormone in Healthy Women of Childbearing Age in Shenzhen Area
文章编号:
1671-7414（2025）06-196-05
作者:
秦晓林a,段 纯a,李俊祥a,高宇驰a,杨 婕a,谭健辉a,马庆林b
南方医科大学附属深圳市妇幼保健院a.妇幼研究所；b.检验科，广东深圳 518028
Author(s):
QIN Xiaolina，DUAN Chuna，LI Junxianga，GAO Yuchia，YANG Jiea，TAN Jianhuia，MA Qinglinb
a. Department of Gynecology; b. Department of Clinical Laboratory, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University，Guangdong Shenzhen 518028，China
关键词:
Hoffmann法; 间接法; 抗缪勒氏管激素; 参考区间; 深圳地区
分类号:
R446.1
DOI:
10.3969/j.issn.1671-7414.2025.06.036
文献标志码:
A
摘要:
目的?采用以数学统计为基础的间接法建立深圳地区育龄期健康女性血清抗缪勒氏管激素（AMH）参考区间并验证。方法收集深圳市妇幼保健院2017～2023年在健康科和体检科体检的21～50岁女性AMH检测数据。以五岁跨度进行分组，首先对每组数据进行正态性检验，对非正态数据采用四分位间距法进行离群值的剔除，然后通过间接法（Hoffmann法）建立不同年龄段AMH的参考区间，并进行验证。与试剂说明书参考区间进行比较并分析血清AMH水平和年龄的相关性。结果育龄期女性血清AMH与年龄的相关系数为-0.642,差异具有统计学意义（P＜0.001）。比较6个年龄组女性血清AMH水平总体分布，差异具有统计学意义（H=28392.655，P＜0.05）；随着年龄的增长,AMH水平呈下降趋势。21～25，26～30，31～35，36～40，41～45和46～50岁血清AMH参考区间分别为0.92～11.3，0.68～9.43，0.38～7.51，0.12～6.93，＜4.42和＜1.83ng/ml，且各组参考区间均通过验证。相比厂家提供的年龄组，该研究年龄分组更细化，参考区间的范围更窄，对临床和实验室的解释更为准确。结论该研究首次采用Hoffmann间接法建立了深圳地区21～50岁健康女性AMH参考区间。该参考区间比较符合实际情况，是一种简单可靠的获取模式，适用于临床实验室的推广和应用。
Abstract:
Objective To establish and validate the reference interval for serum anti-Müllerian hormone (AMH) in healthy women of childbearing age in Shenzhen using an indirect method based on mathematical statistics. Methods Collected the AMH data for women aged 21～50 in outpatient and physical examination populations in Shenzhen Maternity and Child Health Care Hospital from 2017 to 2023. Grouping by age range of five, first performed normality tested on each group of data, and used the interquartile range method to remove outliers for non-normal data. Then established reference intervals for AMH in different age groups through indirect method (Hoffmann method) and verified them. Compared with the reference interval of the reagent instructions and analyzed the correlation be-tween serum AMH levels and age. Results The correlation coefficient between serum AMH and age in women of childbearing age was-0.642，and the difference was statistically significant (P<0.001). The distribution of AMH levels among women in six age groups was compared, and the difference was statistically significant(H=28 392.655, P<0.05)，and AMH levels showed a decreasing trend with age. The reference range of serum AMH at ages 21～25，26～30，31～35，36～40，41～45 and 46～50 yesrs were 0.92～11.30, 0.68～9.43， 0.38～7.51，0.12～6.93，<4.42 and <1.83ng/ml, respectively, and all reference intervals in each group had been validated. Compared to the age groups provided by the manufacturer, the age groups in this study are more refined, the reference range was narrower, and the interpretation of clinical and laboratory data was more accurate. Conclusion This study used Hoffmann’s indirect method for the first time to establish a reference range for AMH in women aged 21～50 years in Shenzhen. The reference interval established is more in line with the actual situation and is a simple and reliable acquisition mode, suitable for promotion and wide application in clinical labo-ratories.

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备注/Memo

备注/Memo:
基金项目：深圳市科技计划项目（JCYJ20220530155213031）；广东省基础与应用基础研究基金项目（2020A1515010590）。
作者简介：秦晓林（1986-），女，硕士，副主任技师。研究方向：临床生化检验，E-mail：qinxiaolin2009@126.com。
通讯作者：马庆林（1986-），男，博士，主任技师，硕士研究生导师。研究方向：分子生物学，E-mail：mql1986@163.com。
更新日期/Last Update: 2025-11-15