[1]郁芳芳,谢伟国,许 群.哮喘患者血清lncRNA HOTTIP和RMRP水平表达及与气道重塑的相关性分析[J].现代检验医学杂志,2026,41(01):70-74+85.[doi:10.3969/j.issn.1671-7414.2026.01.014]
　YU Fangfang,XIE Weiguo,XU Qun.Expression of Serum lncRNA HOTTIP and RMRP in Asthma Patients and Their Correlation Analysis with Airway Remodeling[J].Journal of Modern Laboratory Medicine,2026,41(01):70-74+85.[doi:10.3969/j.issn.1671-7414.2026.01.014]
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哮喘患者血清lncRNA HOTTIP和RMRP水平表达及与气道重塑的相关性分析()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年01期
页码:
70-74+85
栏目:
论著
出版日期:
2026-01-15

文章信息/Info

Title:
Expression of Serum lncRNA HOTTIP and RMRP in Asthma Patients and Their Correlation Analysis with Airway Remodeling
文章编号:
1671-7414（2026）01-070-06
作者:
郁芳芳，谢伟国，许 群
江阴市人民医院呼吸与危重症医学科，江苏江阴 214400
Author(s):
YU Fangfang，XIE Weiguo，XU Qun
Department of Respiratory and Critical Care Medicine, Jiangyin People’s Hospital, Jiangsu Jiangyin 214400, China
关键词:
哮喘; 长链非编码核糖核酸HOXA末端转录本反义RNA; 线粒体RNA处理核糖核酸内切酶RNA组份; 气道重塑
分类号:
R562.25；R392.11
DOI:
10.3969/j.issn.1671-7414.2026.01.014
文献标志码:
A
摘要:
目的?探究哮喘患者血清长链非编码RNA（lncRNA）HOXA末端转录本反义RNA（HOTTIP）和线粒体RNA处理核糖核酸内切酶RNA组份（RMRP）表达变化及其与气道重塑的相关性。方法选取2022年1月～2024年6月江苏省江阴市人民医院呼吸与危重症医学科收治的急性发作期哮喘患者72例为急性发作期组，同期门诊随访哮喘缓解期患者70例为缓解期组，健康体检者142例为健康对照组。采用实时荧光定量聚合酶链反应（qRT-PCR）检测血清lncRNAHOTTIP和RMRP水平。采用多排螺旋CT测量患者肺内气道的内径（L）、外径（D），计算气道壁厚度与气道外径比值（T/D）和管壁面积占支气道总横截面积百分比（WA%）。采用肺功能检测仪检测第一秒用力呼气量（FEV1）、最高呼吸气流（PEF）水平。Spearman相关性分析哮喘患者血清lncRNAHOTTIP和RMRP水平与哮喘控制测试（ACT）评分的关系。Pearson相关性分析哮喘患者血清lncRNAHOTTIP和RMRP水平以及与肿瘤坏死因子-α（TNF-α）、γ干扰素（IFN-γ）、白细胞介素-18（IL-18）水平、气道重塑以及肺功能指标的关系。结果与健康对照组相比，急性发作期组和缓解期组IFN-γ水平显著降低（t=14.161，8.886），TNF-α、IL-18、lncRNAHOTTIP（1.32±0.15、1.15±0.15vs1.01±0.14）和RMRP（1.27±0.14、1.16±0.14vs1.02±0.13）水平显著升高（t=9.345~28.361）；与缓解期组相比，急性发作期组ACT评分、IFN-γ水平显著降低(t=13.344,4.475)，TNF-α、IL-18、lncRNAHOTTIP和RMRP水平显著升高（t=6.861~15.675），差异具有统计学意义（均P<0.05）。与健康对照组相比，急性发作期组和缓解期组FEV1、PEF水平显著降低（t=13.346~55.694），T/D、WA%水平显著升高（t=19.145~33.035）；与缓解期组相比，急性发作期组FEV1、PEF水平显著降低（t=21.802、21.446），T/D、WA%水平显著升高（t=9.994、10.082），差异具有统计学意义（均P<0.05）。Spearman相关性分析显示哮喘患者血清lncRNAHOTTIP和RMRP水平与ACT评分呈负相关（r=-0.614、-0.399，均P<0.001）。Pearson相关性分析显示，哮喘患者血清lncRNAHOTTIP和RMRP水平呈正相关（r=0.625，P<0.05）。哮喘患者血清lncRNAHOTTIP和RMRP水平与TNF-α（r=0.658、0.632）、IL-18（r=0.584、0.586）、T/D（r=0.604、0.631）、WA%（r=0.597、0.588）水平呈正相关，与IFN-γ（r=-0.587、-0.517）、FEV1（r=-0.568、-0.577）、PEF（r=-0.634、-0.627）水平呈负相关（均P<0.05）。结论哮喘患者血清lncRNAHOTTIP和RMRP水平均呈高表达，与气道重塑和肺功能指标变化有关。
Abstract:
Objective To investigate the expression changes of lncRNA HOTTIP and RMRP in the serum of patients with asthma, and their correlation with airway remodeling. Methods From January 2022 to June 2024, 72 patients with acute attack admitted to Jiangyin People’s Hospital were selected as the acute attack group, 70 patients with asthma remission during outpatient fol-low-up during the same period were selected as the remission group, and 142 healthy volunteers who underwent physical exam-ination in the hospital were selected as the healthy control group. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was applied to detect serum lncRNA HOTTIP and RMRP levels. Multi row spiral CT was applied to measure its inner diameter (L), outer diameter (D), and the ratio of airway wall thickness to airway outer diameter (T/D) and the percentage of wall area to total cross-sectional area of the airway (WA%) were calculated. The pulmonary function tester was used to measure the forced expiratory volume in first second (FEV1) and the peak expiratory flow (PEF).Used Spearman correlation analysis to investi-gate the relationship between serum lncRNA HOTTIP and RMRP levels and asthma control test (ACT) scores in asthma patients, Pearson correlation analysis was used to investigate the levels of serum lncRNA HOTTIP and RMRP in asthma patients, as well as their relationship with tumor necrosis factor-α (TNF-α), interferon gamma (IFN-γ), interleukin-18 （1L-18） levels, airway remodeling and lung function indicators. Results Compared with the healthy control group, IFN-γ levels in acute at-tack and remission groups were significantly reduced（t=14.161,8.886）. TNF-α, IL-18, lncRNA HOTTIP (1.32±0.15, 1.15±0.15 vs 1.01±0.14) and RMRP (1.27±0.14, 1.16±0.14 vs 1.02±0.13) levels were significantly increased (t=9.345~28.361). The levels of ACT score and IFN-γ in the acute attack group were significantly lower than those in the remission group(t=13.344, 4.475). Levels of TNF-α, IL-18, lncRNA HOTTIP and RMRP were significantly increased (t=6.861~15.675),and the differences were statistically sig-nificant(all P<0.05). Compared with the healthy control group, the levels of FEV1 and PEF in acute attack and remission groups were significantly decreased(t=13.346~55.694), and the levels of T/D and WA% were significantly increased(t=19.145~33.035). Compared with remission group, the levels of FEV1 and PEF in acute attack group were significantly decreased(t=21.802, 21.446), and the levels of T/D and WA% were significantly increased (t=9.994, 10.082), the differences were statistically significant(all P<0.05). Spearman cor-relation analysis showed that the levels of serum lncRNA HOTTIP and RMRP in asthma patients were negatively correlated with ACT scores (r=-0.614, -0.399, all P<0.001). Pearson correlation analysis showed that serum lncRNA HOTTIP and RMRP levels were posi-tively correlated in patients with asthma (r=0.625, P<0.05). serum lncRNA HOTTIP and RMRP levels in patients with asthma were correlated with TNF-α (r=0.658, 0.632), IL-18 (r=0.584, 0.586), T/D (r=0.604, 0.631), WA% (r =0.597, 0.588) levels were positively correlated with IFN-γ (r=-0.587, -0.517), FEV1 (r=-0.568, -0.577), PEF (r =-0.634, -0.627) levels (all P<0.05). Conclusions Se-rum lncRNA HOTTIP and RMRP levels are highly expressed in asthma patients and are associated with airway remodeling and chang-es in lung function indicators.

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备注/Memo

备注/Memo:
作者简介：郁芳芳（1984-），女，学士，副主任医师，研究方向：肺部感染性疾病、肺部恶性肿瘤，E-mail：y1ny9f9e@163.com。
通讯作者：许群（1986-），女，硕士，副主任医师，研究方向：肺部感染性疾病、肺部恶性肿瘤，E-mail：858218554@qq.com。
更新日期/Last Update: 2026-01-15